Arctiin regulates collagen type 1α chain 1 mRNA expression in human dermal fibroblasts via the miR-378b-SIRT6 axis

Kim, Karam; An, Sungkwan; Choi, Byung Gon; Joo, Da-Hye; Choi, Young Min; Ahn, Kyu Joong; An, In‐Sook; Jun, Hwa

doi:10.3892/mmr.2017.7679

Cited by 6 publications

(8 citation statements)

References 37 publications

(36 reference statements)

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“…A previous study has shown that miR-378b is expressed in normal human dermal fibroblasts, and circulating miR-378b is a sensitive biomarker for photo-aging (Joo et al, 2017). It was also found to protect dermal fibroblasts from UVB-inhibited collagen I by targeting SIRT6 (Kim et al, 2017). However, the role of miR-378b in the pathogenesis of hepatic insulin resistance remains elusive.…”

Section: Discussionmentioning

confidence: 99%

miR-378b Regulates Insulin Sensitivity by Targeting Insulin Receptor and p110α in Alcohol-Induced Hepatic Steatosis

Zhong

Cheng

et al. 2020

Front. Pharmacol.

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Insulin resistance has been implicated in alcoholic liver disease. A previous study has shown that microRNAs (miRNAs) play a major role in the production, secretion, and function of insulin. MiRNAs are capable of repressing multiple target genes that in turn negatively regulate various physiological and pathological activities. However, current information on the biological function of miRNAs in insulin resistance is limited. The goal of the present study was to elucidate the role of miR-378b in alcohol-induced hepatic insulin resistance and its underlying mechanism. This study has observed that miR-378b is upregulated in National Institute on Alcohol Abuse and Alcoholism (NIAAA) alcoholic mouse models as well as in ethanol-induced L-02 cells in vitro. Furthermore, miR-378b overexpression impaired the insulin signaling pathway, and inhibition of miR-378b improved insulin sensitivity in vivo and in vitro. A mechanistic study revealed that IR and p110a are direct targets of miR-378b. Together, these results suggest that miR-378b controls insulin sensitivity by targeting the insulin receptor (IR) as well as p110a and possibly play an inhibitory role in the development of insulin resistance, thereby providing insights into the development of novel diagnostic and treatment methods.

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Section: Discussionmentioning

confidence: 99%

miR-378b Regulates Insulin Sensitivity by Targeting Insulin Receptor and p110α in Alcohol-Induced Hepatic Steatosis

Zhong

Cheng

et al. 2020

Front. Pharmacol.

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“…This subsequently represses the mRNA expression levels of a-1-type 1 collagen (COL1A1) by disrupting SIRT6 activity ( 77 , 78 ). Arctin, a lignin derived from Arctium lappa , downregulates microRNA378b, preventing UVB-induced reduction in COL1A1 and restoring SIRT6 activity ( 78 ). Recently, in vitro studies by Lee et al revealed that UV-irradiated dermal fibroblasts had lowered SIRT2 and SIRT3 mRNA and protein levels ( 79 ).…”

Section: Fibroblast Senescencementioning

confidence: 99%

“…Specifically, microRNA-378b is induced in UVB-exposed human dermal fibroblasts. This subsequently represses the mRNA expression levels of a-1-type 1 collagen (COL1A1) by disrupting SIRT6 activity (77,78). Arctin, a lignin derived from Arctium lappa, downregulates microRNA378b, preventing UVB-induced reduction in COL1A1 and restoring SIRT6 activity (78) ), hydrogen peroxide (H 2 O 2 ), hydroxyl radicals (•OH), and singlet oxygen ( 1 O 2 ).…”

Section: Sirtuins and Stress-induced Senescencementioning

confidence: 99%

“…This subsequently represses the mRNA expression levels of a-1-type 1 collagen (COL1A1) by disrupting SIRT6 activity (77,78). Arctin, a lignin derived from Arctium lappa, downregulates microRNA378b, preventing UVB-induced reduction in COL1A1 and restoring SIRT6 activity (78) ), hydrogen peroxide (H 2 O 2 ), hydroxyl radicals (•OH), and singlet oxygen ( 1 O 2 ). These are all produced as metabolic by-products from various sources including mitochondrial electron transport chain, the peroxisome, and UV-mediated accumulation (81,82).…”

Section: Sirtuins and Stress-induced Senescencementioning

confidence: 99%

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The role of sirtuins in dermal fibroblast function

et al. 2023

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The sirtuins are a family of seven proteins that perform a variety of dermatological functions and help maintain both the structure and function of the skin. More specifically, the sirtuins have been shown to be altered in multiple dermal cell types including dermal fibroblasts. The functions of dermal fibroblasts are extensive, and include playing a significant role in wound healing as well as helping to maintain the integrity of the skin. As dermal fibroblasts age, they can undergo a state of permanent cell cycle arrest, known as cellular senescence. This senescent process can occur as a result of various stressors, including oxidative stress, ultraviolet radiation -induced stress, and replicative stress. In recent years, there has been a growing interest in both enhancing the cutaneous fibroblast’s ability to facilitate wound healing and altering fibroblast cellular senescence. Thus, in this review, we examine the relationship between sirtuin signaling and dermal fibroblasts to understand how this family of proteins may modulate skin conditions ranging from the wound healing process to photocarcinogenesis associated with fibroblast senescence. Additionally, we offer supporting data from experiments examining the relationship between fibroblast senescence and sirtuin levels in an oxidative stress model indicating that senescent dermal fibroblasts exhibit diminished sirtuin levels. Furthermore, we survey the research on the role of sirtuins in specific dermatological disease states that where dermal fibroblast function has been implicated. Finally, we conclude with outlining potential clinical applications of sirtuins in dermatology. In sum, we find that the literature on the involvement of sirtuins in dermal fibroblasts is limited, with research still in its early stages. Nevertheless, intriguing preliminary findings merit additional investigation into the clinical implications of sirtuins in dermatology.

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“…miR-128 inhibits osteoblast differentiation in osteoporosis by downregulating SIRT6 expression, thus accelerating the development of osteoporosis [ 19 ]. miR-378b represses the mRNA expression levels of COL1A1 via interference with SIRT6 in human dermal fibroblasts [ 20 , 21 ].…”

Section: Collagen Formationmentioning

confidence: 99%

The Regulation of Collagen Processing by miRNAs in Disease and Possible Implications for Bone Turnover

Lehmann

Guderska

Kałek

et al. 2021

IJMS

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This article describes several recent examples of miRNA governing the regulation of the gene expression involved in bone matrix construction. We present the impact of miRNA on the subsequent steps in the formation of collagen type I. Collagen type I is a main factor of mechanical bone stiffness because it constitutes 90–95% of the organic components of the bone. Therefore, the precise epigenetic regulation of collagen formation may have a significant influence on bone structure. We also describe miRNA involvement in the expression of genes, the protein products of which participate in collagen maturation in various tissues and cancer cells. We show how non-collagenous proteins in the extracellular matrix are epigenetically regulated by miRNA in bone and other tissues. We also delineate collagen mineralisation in bones by factors that depend on miRNA molecules. This review reveals the tissue variability of miRNA regulation at different levels of collagen maturation and mineralisation. The functionality of collagen mRNA regulation by miRNA, as proven in other tissues, has not yet been shown in osteoblasts. Several collagen-regulating miRNAs are co-expressed with collagen in bone. We suggest that collagen mRNA regulation by miRNA could also be potentially important in bone metabolism.

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Arctiin regulates collagen type 1α chain 1 mRNA expression in human dermal fibroblasts via the miR-378b-SIRT6 axis

Cited by 6 publications

References 37 publications

miR-378b Regulates Insulin Sensitivity by Targeting Insulin Receptor and p110α in Alcohol-Induced Hepatic Steatosis

miR-378b Regulates Insulin Sensitivity by Targeting Insulin Receptor and p110α in Alcohol-Induced Hepatic Steatosis

The role of sirtuins in dermal fibroblast function

The Regulation of Collagen Processing by miRNAs in Disease and Possible Implications for Bone Turnover

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