Determination of the bioavailability of selected pharmaceutical residues in fish plasma using liquid chromatography coupled to tandem mass spectrometry

Larabie, S. Poirier; Houde, Magali; Gagnon, Christian

doi:10.1016/j.chroma.2017.09.055

Cited by 10 publications

(2 citation statements)

References 20 publications

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“…The first one is made of 1.6 µm solid-core particles, while the second one is made of 1.7 µm porous particles having a bridged ethyl-siloxane silica hybrid (BEH) structure. Both are expected to improve resolution and efficiency of separation and have been successfully used for determination of pharmaceuticals (except sotalol) in water matrices [17,27,34,35]. These columns were assessed with a gradient elution 0.1% ammonium formate (AF) used as aqueous eluent, while 0.1% to 0.3% AF in ACN were tested as organic eluents, based on the assumption that shifting pH to acid will serve a better separation of targeted molecules.…”

Section: Methods Developmentmentioning

confidence: 99%

Fast UHPLC-MS/MS for the Simultaneous Determination of Azithromycin, Erythromycin, Fluoxetine and Sotalol in Surface Water Samples

et al. 2021

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Chromatographic development for the determination of pharmaceuticals in environmental water samples is particularly challenging when the analytes have significantly different physico-chemical properties (solubility, polarity, pKa) often requiring multiple chromatographic methods for each active component. This paper presents a method for the simultaneous determination of azithromycin, erythromycin (antibiotics), fluoxetine (anti-depressant) and sotalol (b-blocker) in surface waters by ultra-high-performance liquid chromatography coupled with ultra-high-resolution time-of-flight mass spectrometry. These pharmaceuticals—presenting a broad spectrum of polarity (0.24 ≤ log Kow ≤ 4.05)—were separated on a C-18 analytical column, after a simple filtration step for freshwater samples or after a liquid–liquid extraction with Methyl-tertio-butyl ether (MTBE) for seawater samples. The optimized separation method (in terms of nature of column and eluent, elution gradient, and of mass spectrometric parameters), enable one to reach limits of detection ranging between 2 and 7 ng L−1 and limits of quantification between 7 and 23 ng L−1 for the four targeted molecules, within a three minute run. This method was validated using samples collected from three different surface waters in Lebanon (freshwater and seawater) and analytical results were compared with those obtained in surface waters sampled in a French river, equivalent in terms of human activities. Using this method, we report the highest concentration of pharmaceuticals found in surface water (up to 377 ng L−1 and 268 ng L−1, respectively, for azithromycin and erythromycin, in the Litani river, Lebanon).

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Section: Methods Developmentmentioning

confidence: 99%

Fast UHPLC-MS/MS for the Simultaneous Determination of Azithromycin, Erythromycin, Fluoxetine and Sotalol in Surface Water Samples

et al. 2021

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“…Concerning fish and crayfish, measurements are biased toward certain species. In pike, measurements have only been made in plasma (Larabie et al, 2017). No measurements of pharmaceuticals have been made in signal crayfish and common rudd.…”

Section: Introductionmentioning

confidence: 99%

Pharmaceuticals in Municipal Wastewater – Two Case Studies of Uptake in Fish and Crayfish (Pacifastacus leniusculus) in Aquaria Experiment and In-field Sampling

Waldetoft,

Esfahani,

Viktor

et al. 2023

EREM

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In the receiving areas of effluents from municipal wastewater treatment plants (WWTPs), aquatic organisms are threatened by adverse effects due to exposure to pharmaceutical residues. To elucidate the uptake of pharmaceuticals in fish, measurements were made in bile of brown trout (Salmo trutta) exposed in aquaria to 100% effluent water and in muscle, liver, kidney, and bile in northern pike (Esox lucius), European perch (Perca fluviatilis) and common rudd (Scardinius erythrophthalmus) from a lake receiving municipal wastewater. Pharmaceuticals were also measured in hepatopancreas of signal crayfish (Pacifastacus leniusculus). In addition to the measurements in fish and crayfish, pharmaceuticals were measured in the effluent, upstream and downstream of the WWTPs. In effluent water, pharmaceuticals were detected in the µg/L range, with the highest concentrations being of commonly prescribed NSAIDs and hypertension drugs, such as diclofenac, ibuprofen, naproxen, losartan, and metoprolol. However, the differences in concentrations between different sampling occasions were high, indicating a need for repeated sampling to obtain representative average concentrations. Pharmaceuticals in fish samples showed strong tendencies to species and tissue-specific partitioning. Levels of diclofenac in the brown trout bile were within the range of 4–16 µg/g w.w and naproxen within 37–170 ng/g w.w, while for all other pharmaceuticals, they were below detection limits. Several other pharmaceuticals were present in a similar order of magnitude in the effluent as diclofenac, suggesting diclofenac has a strong partitioning to trout bile. In the wild fish, the highest number of detected pharmaceuticals and the highest levels were observed in kidney of pike. Diclofenac in pike kidney was at most 37 ng/g w.w, followed by propranolol (20 ng/g w.w) and losartan (18 ng/g w.w). In crayfish, no pharmaceuticals were detected. The results suggest that the kidney of pike is a suitable tissue for evaluating pharmaceuticals in fish, while hepatopancreas of signal crayfish is not.

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Occurrence, bioconcentration, and human health risks of pharmaceuticals in biota in the Sea of Marmara, Türkiye

et al. 2023

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Determination of the bioavailability of selected pharmaceutical residues in fish plasma using liquid chromatography coupled to tandem mass spectrometry

Cited by 10 publications

References 20 publications

Fast UHPLC-MS/MS for the Simultaneous Determination of Azithromycin, Erythromycin, Fluoxetine and Sotalol in Surface Water Samples

Fast UHPLC-MS/MS for the Simultaneous Determination of Azithromycin, Erythromycin, Fluoxetine and Sotalol in Surface Water Samples

Pharmaceuticals in Municipal Wastewater – Two Case Studies of Uptake in Fish and Crayfish (Pacifastacus leniusculus) in Aquaria Experiment and In-field Sampling

Occurrence, bioconcentration, and human health risks of pharmaceuticals in biota in the Sea of Marmara, Türkiye

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