Estimating HPV DNA Deposition Between Sexual Partners Using HPV Concordance, Y Chromosome DNA Detection, and Self-reported Sexual Behaviors

Malagón, Talía; Burchell, Ann; El‐Zein, Mariam; Guénoun, Julie; Tellier, Pierre-Paul; Coutlée, François; Franco, Eduardo L.

doi:10.1093/infdis/jix477

Cited by 26 publications

(24 citation statements)

References 29 publications

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“…A recent case report, for example, found an increase in Lactobacillus abundance in the penile microbiota following vaginal sex [29]. Further, a significant proportion of HPV DNA detections may represent depositions from recent unprotected sex with an infected partner [50]. Differences in the time since the last sex act and nature of the last sexual exposure could therefore have impacted the findings reported in the present study.…”

Section: Discussionmentioning

confidence: 64%

The penile microbiota of Black South African men: relationship with human papillomavirus and HIV infection

et al. 2020

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Background: To date, the microbiota of the human penis has been studied mostly in connection with circumcision, HIV risk and female partner bacterial vaginosis (BV). These studies have shown that male circumcision reduces penile anaerobic bacteria, that greater abundance of penile anaerobic bacteria is correlated with increased cytokine levels and greater risk of HIV infection, and that the penile microbiota is an important harbour for BVassociated bacteria. While circumcision has been shown to significantly reduce the risk of acquiring human papillomavirus (HPV) infection, the relationship of the penile microbiota with HPV is still unknown. In this study, we examined the penile microbiota of HPV-infected men as well as the impact of HIV status. Results:The penile skin microbiota of 238 men from Cape Town (South Africa) were profiled using Illumina sequencing of the V3-V4 hypervariable regions of the 16S rRNA gene. Corynebacterium and Prevotella were found to be the most abundant genera. Six distinct community state types (CSTs) were identified. CST-1, dominated by Corynebacterium, corresponded to less infections with high-risk HPV (HR-HPV) relative to CSTs 2-6. Men in CST-5 had greater relative abundances of Prevotella, Clostridiales, and Porphyromonas and a lower relative abundance of Corynebacterium. Moreover, they were significantly more likely to have HPV or HR-HPV infections than men in CST-1. Using a machine learning approach, we identified greater relative abundances of the anaerobic BV-associated bacteria (Prevotella, Peptinophilus, and Dialister) and lower relative abundance of Corynebacterium in HR-HPV-infected men compared to HR-HPV-uninfected men. No association was observed between HIV and CST, although the penile microbiota of HIV-infected men had greater relative abundances of Staphylococcus compared to HIVuninfected men.(Continued on next page) Conclusions: We found significant differences in the penile microbiota composition of men with and without HPV and HIV infections. HIV and HR-HPV infections were strongly associated with greater relative abundances of Staphylococcus and BV-associated bacterial taxa (notably Prevotella, Peptinophilus and Dialister), respectively. It is possible that these taxa could increase susceptibility to HIV and HR-HPV acquisition, in addition to creating conditions in which infections persist. Further longitudinal studies are required to establish causal relationships and to determine the extent of the effect.

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Section: Discussionmentioning

confidence: 64%

The penile microbiota of Black South African men: relationship with human papillomavirus and HIV infection

et al. 2020

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“…This may have been a chance finding, due to the low number of vaccinated women who tested HPV 6/11/16/18 positive. It may also indicate that vaccination prevents persistence of HPV infections, and/or that such short-lasting, clearing HPV detections are not "true" infections of epithelial cells, but for example deposition from the sexual partner (20,21). Vaccinated women who tested positive for HPV 6, 11, 16, or 18 also had lower type-specific viral loads than unvaccinated women who were positive for these types.…”

Section: Discussionmentioning

confidence: 99%

“…Second, incident HPV infections may be rapidly cleared by the immune system in vaccinated women. Third, it is also possible that these low viral load infections in vaccinated women do not represent true infections, but HPV DNA deposition from recent sexual activity with male partners (20). Some evidence for cross-type protection was found.…”

Section: Discussionmentioning

confidence: 99%

Vaccination of Young Women Decreases Human Papillomavirus Transmission in Heterosexual Couples: Findings from the HITCH Cohort Study

Wissing

Burchell

El‐Zein

et al. 2019

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: Vaccination against human papillomaviruses (HPV) prevents HPV infections and, consequently, cervical lesions. However, the effect of vaccination on HPV transmission within couples is unknown. Methods: We used data from HITCH, a prospective cohort study of heterosexual couples (women ages 18-24 years) in Montreal, 2005 to 2013. Vaccination history was selfreported. Genital samples were tested for HPV DNA by PCR (linear array). Type-specific viral loads were quantified using real-time PCR. OR and HR were estimated using multilevel mixed-effects logistic regression and a parametric model for interval-censored survival-time data, respectively. Differences in viral loads were evaluated using the Friedman ANOVA test. Results: Among 497 couples, 12, 16, and 35 women received 1, 2, or 3 vaccination doses at baseline, respectively. Median age at vaccination was 18 years. Most women (92.1%) had their first coitus before vaccination. At baseline, partner concordance of persistent HPV6/11/16/18 infections was lower in vaccinated than unvaccinated women [adjusted OR ¼ 0.10; 95% confidence interval (CI), 0.01-0.65] but not for non a7/a9/a10-HPV types (adjusted OR ¼ 1.00; 95% CI, 0.44-2.29). Incidence of persistent a7/a9/a10 HPV types in women was inversely associated with vaccination status at baseline (adjusted HR ¼ 0.12; 95% CI, 0.03-0.47). Likewise, male partners of vaccinated women had a lower incidence of a7/a9/a10 HPV infections (adjusted OR ¼ 0.22; 95% CI, 0.05-0.95). Vaccinated women with HPV 6/11/16/18 infections had lower viral loads (P ¼ 0.001) relative to unvaccinated women. Conclusions: Vaccination of sexually active women significantly reduced transmission of a7/a9/a10 HPV types in heterosexual couples. Impact: These results underscore and quantify the positive effect of HPV vaccination on HPV transmission within heterosexual couples.

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“…We examined whether sexual risk factors and a biomarker, Y chromosome DNA, were associated with genital HPV partner concordance using baseline genital specimens [33]. HPV DNA Y chromosome DNA predicted type-specific HPV concordance in univariate analyses, but in multivariable models the independent predictors of concordance were days since last vaginal sex and condom use.…”

Section: Y Chromosome Detection and Concordance Of Genital Hpv Infectmentioning

confidence: 99%

Human Papillomavirus Infection and Transmission Among Couples Through Heterosexual Activity (HITCH) Cohort Study: Protocol Describing Design, Methods, and Research Goals (Preprint)

El‐Zein¹,

Coutlée²,

Tellier³

et al. 2018

Preprint

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Estimating HPV DNA Deposition Between Sexual Partners Using HPV Concordance, Y Chromosome DNA Detection, and Self-reported Sexual Behaviors

Abstract: A substantial proportion of HPV DNA detections may be depositions due to recent unprotected vaginal sex.

Cited by 26 publications

References 29 publications

The penile microbiota of Black South African men: relationship with human papillomavirus and HIV infection

The penile microbiota of Black South African men: relationship with human papillomavirus and HIV infection

Vaccination of Young Women Decreases Human Papillomavirus Transmission in Heterosexual Couples: Findings from the HITCH Cohort Study

Human Papillomavirus Infection and Transmission Among Couples Through Heterosexual Activity (HITCH) Cohort Study: Protocol Describing Design, Methods, and Research Goals (Preprint)

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