Antibacterial activity of a novel antimicrobial peptide [W7]KR12-KAEK derived from KR-12 against Streptococcus mutans planktonic cells and biofilms

Abstract

The aims of this study were to describe the synthesis of a novel synthetic peptide based on the primary structure of the KR-12 peptide and to evaluate its antimicrobial and anti-biofilm activities against Streptococcus mutans. The antimicrobial effect of KR-12 and [W]KR12-KAEK was assessed by determining the minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations. The evaluation of anti-biofilm activity was assessed through total biomass quantification, colony forming unit counting and scanning electron microscopy. [W]KR12-KAEK showed MIC and MBC values ranging from 31.25 to 7.8 and 62.5 to 15.6 μg ml, respectively. Furthermore, [W7]KR12-KAEK significantly reduced biofilm biomass (50-100%). Regarding cell viability, [W]KR12-KAEK showed reductions in the number of CFUs at concentrations ranging from 62.5 to 7.8 μg ml and 500 to 62.5 μg ml with respect to biofilm formation and preformed biofilms, respectively. SEM micrographs of S. mutans treated with [W]KR12-KAEK suggested damage to the bacterial surface. [W]KR12-KAEK is demonstrated to be an antimicrobial agent to control microbial biofilms.

Synthetic antigen-binding fragments (Fabs) against S. mutans and S. sobrinus inhibit caries formation

“…mutans and S . sobrinus biofilm formation were examined using the microdilution method 41 . Briefly, overnight culture of S .…”

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

“…Jannadi et al [89] synthesized Pep19-2.5 and Pep19-4LF and assessed that they inhibited S. mutans growth and biofilm formation. Similarly, Zhang et al [39] designed DPS-PI, Liang et al [90] designed LR-10, Da Silva et al [91] designed [W7]KR12-KAEK, and all of their antibacterial activities were evaluated by assessing the inhibition of S. mutans growth and biofilm biomass, furthermore assessing the destruction to biofilm morphology and the damage to the bacterial surface via scanning electron microscopy. In this current study, GERM CLEAN showed capabilities on reducing the initial adherence and disrupting the biofilm formation of S. mutans.…”

A novel non‐cytotoxic synthetic peptide, Pug‐1, exhibited an antibiofilm effect onStreptococcus mutansadhesion

“…b–d). The differences in peptide length, hydrophobicity and net charges between the different Pug peptides may explain the different cytotoxicity of the Pug peptides (da Silva et al ).…”