Behavioral and molecular analyses of olfaction-mediated avoidance responses of Rana ( Lithobates ) catesbeiana tadpoles: Sensitivity to thyroid hormones, estrogen, and treated municipal wastewater effluent

Heerema, Jody L.; Jackman, Kevin W.; Miliano, Rachel C.; Li, Linda; Zaborniak, Tristan; Veldhoen, Nik; Aggelen, Graham van; Parker, Wayne; Pyle, Greg G.; Helbing, Caren C.

doi:10.1016/j.yhbeh.2017.09.016

Cited by 12 publications

(9 citation statements)

References 56 publications

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“…The molecular effects of up to 72 h of methimazole exposure on early prometamorphic X. laevis tadpoles were queried by qPCR analysis of known TH-regulated genes. Zhang et al found a significant decrease in thra and app gene expression in the brain; a decrease in thra and increase in ipo and krt1 mRNAs in the hindlimb; and a decrease in ipo transcripts in the tail (Table 2) (36, 38, 39). Helbing et al used cDNA arrays to further evaluate the pathways affected by methimazole in X. laevis tadpoles (Table 2) (32, 34).…”

Section: Pharmaceuticals and Personal Care Productsmentioning

confidence: 99%

Section: Pharmaceuticals and Personal Care Productsmentioning

confidence: 99%

“…Exposure to T 3 is also associated with behavioral changes in which tadpoles lose the ability to detect a predator cue (36). Surprisingly, comparable T 4 exposures had no effect on this behavioral endpoint (36). Molecular analyses of the olfactory epithelium using qPCR and RNA-seq methods revealed that this tissue was extraordinarily sensitive to both hormones and, while many gene responses were shared between the two hormones, a substantial number were unique to each hormone with T 3 significantly affecting a true14 more contigs than T 4 (6, 7).…”

Section: Pharmaceuticals and Personal Care Productsmentioning

confidence: 99%

“…Molecular analyses of the olfactory epithelium using qPCR and RNA-seq methods revealed that this tissue was extraordinarily sensitive to both hormones and, while many gene responses were shared between the two hormones, a substantial number were unique to each hormone with T 3 significantly affecting a true14 more contigs than T 4 (6, 7). Notable differences in sensory perception, potassium ion transport, DNA repair, mitochondrial energetics and transcription/RNA processing gene ontologies provide some insight into the different effects of these hormones (36). These studies accentuate that the two TH contaminants should be treated separately when looking at responses to environmentally-relevant levels of THs.…”

Section: Pharmaceuticals and Personal Care Productsmentioning

confidence: 99%

“…A primary source of contamination comes from PPCPs in human waste. Although wastewater goes through extensive filtration prior to dispersal, TH disruption still ensues from effluent exposures (36). The TH disruption potential of WWE was first examined in 2009 when Sowers et al found that a 50% dilution of municipal WWE significantly decreased the rate of R. pipiens metamorphosis (Table 4) (186).…”

Section: Complex Mixturesmentioning

confidence: 99%

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Contaminant and Environmental Influences on Thyroid Hormone Action in Amphibian Metamorphosis

et al. 2019

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Aquatic and terrestrial environments are increasingly contaminated by anthropogenic sources that include pharmaceuticals, personal care products, and industrial and agricultural chemicals (i. e., pesticides). Many of these substances have the potential to disrupt endocrine function, yet their effect on thyroid hormone (TH) action has garnered relatively little attention. Anuran postembryonic metamorphosis is strictly dependent on TH and perturbation of this process can serve as a sensitive barometer for the detection and mechanistic elucidation of TH disrupting activities of chemical contaminants and their complex mixtures. The ecological threats posed by these contaminants are further exacerbated by changing environmental conditions such as temperature, photoperiod, pond drying, food restriction, and ultraviolet radiation. We review the current knowledge of several chemical and environmental factors that disrupt TH-dependent metamorphosis in amphibian tadpoles as assessed by morphological, thyroid histology, behavioral, and molecular endpoints. Although the molecular mechanisms for TH disruption have yet to be determined for many chemical and environmental factors, several affect TH synthesis, transport or metabolism with subsequent downstream effects. As molecular dysfunction typically precedes phenotypic or histological pathologies, sensitive assays that detect changes in transcript, protein, or metabolite abundance are indispensable for the timely detection of TH disruption. The emergence and application of ‘omics techniques—genomics, transcriptomics, proteomics, metabolomics, and epigenomics—on metamorphosing tadpoles are powerful emerging assets for the rapid, proxy assessment of toxicant or environmental damage for all vertebrates including humans. Moreover, these highly informative ‘omics techniques will complement morphological, behavioral, and histological assessments, thereby providing a comprehensive understanding of how TH-dependent signal disruption is propagated by environmental contaminants and factors.

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