A new clerodane furano diterpene glycoside from Tinospora cordifolia triggers autophagy and apoptosis in HCT-116 colon cancer cells

Sharma, Neha; Kumar, Ashok; Sharma, Parduman R.; Qayum, Arem; Singh, Shashank; Dutt, Prabhu; Paul, Satya; Gupta, Vivek K.; Verma, Mahendra K.; Satti, Naresh K.; Vishwakarma, Ram A.

doi:10.1016/j.jep.2017.09.034

Cited by 32 publications

(31 citation statements)

References 42 publications

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“…We speculated that not only the flavonoids and polyphenols but also the alkaloids, which may be in TCSE, might have modulated body weight, diet intake, and insulin secretion in diabetic animals. In addition, Sharma et al (Sharma et al, ) recently reported the autophagy promoting effect of clerodane furano diterpene glycoside from T. cordifolia . Autophagy regulates body weight via differentially regulating insulin production and insulin sensitivity (Yamamoto et al, ).…”

Section: Discussionmentioning

confidence: 99%

Tinospora cordifolia preserves pancreatic beta cells and enhances glucose uptake in adipocytes to regulate glucose metabolism in diabetic rats

Sharma

Park

Kim

et al. 2019

Phytotherapy Research

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The purpose of this study was to evaluate the pancreatic beta cell protective and glucose uptake enhancing effect of the water extract of Tinospora cordifolia stem (TCSE) by using rat insulinoma (RIN)-m5F cells and 3 T3-L1 adipocytes. RIN-m5F cells were stimulated with interleukin-1β and interferon-γ, and the effect of TCSE on insulin secretion and cytokine-induced toxicity was measured by ELISA and MTT assay, respectively. The glucose uptake and protein expression were measured by fluorometry and western blotting. Antidiabetic effect of TCSE was measured using streptozotocin-induced diabetic rats. TCSE dose dependently increased cell viability and insulin secretion in RIN-m5F cells. In addition, TCSE increased both the glucose uptake and glucose transporter 4 translocation in 3 T3-L1 adipocytes via PI3K pathway.Finally, TCSE significantly lowered blood glucose and diet intake and increased body weight in streptozotocin-induced diabetic rats. The level of serum insulin and hepatic glycogen was increased, whereas the level of serum triglyceride, total cholesterol, dipeptidyl peptidase-4, and thiobarbituric acid reactive substances was decreased in TCSE-administered rats. TCSE also increased glucose transporter 4 protein expression in the adipose tissue and liver of TCSE-fed diabetic rats. Our results suggested that TCSE preserved RIN-m5F cells from cytokine-induced toxicity and enhanced glucose uptake in 3 T3-L1 adipocytes, which may regulate glucose metabolism in diabetic rats.

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Section: Discussionmentioning

confidence: 99%

Tinospora cordifolia preserves pancreatic beta cells and enhances glucose uptake in adipocytes to regulate glucose metabolism in diabetic rats

Sharma

Park

Kim

et al. 2019

Phytotherapy Research

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“…The methanolic extracts of Tc have shown to exhibit an IC 50 value of less than 100 µg/mL [29]. Sharma N used 1.5 kg of dried, crushed Tc soaked in 4.5 liters and found an IC 50 value of less than 100 µg/mL [6]. Priya M S et al used varying dosage (200, 400 and 600 µg/mL) of Tc, revealing dose-dependent inhibition [7].…”

Section: Dose Effect Of Phytocomponentsmentioning

confidence: 99%

“…The various phytocomponents identified from the studies analyzed in the systematic review included berberine, new clerodane furanodiptherineglycosidae, elligic acid, kaempferol, N-formylannonain, magnoflorine, jatrorrhizine palmatine, 11-hydroxymustakone, cordifolioside A, tinocordiside, yangambin, anthraquinones, terpenoids, saponins and phenol, pyrrole-based small molecules, quercetin and rutin, arabinogalectian, palmatine, clerodane-derived diterpenoids and hexane fractions. These phytocomponents induced anticancer effect via mitochondrial-mediated apoptosis, cytotoxic activity, mutagenic activity, reduction in tumor size, triggering reactive oxygen species, decreased gene expression of the cell cycle, effectively inhibiting cancer proliferation [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23]. The mechanism of action of Tc depends on the phytocomponents used.…”

Section: Phytocomponents and Its Mechanism Of Action Against Cancer Cmentioning

confidence: 99%

“…The mechanism of action of Tc depends on the phytocomponents used. The new clerodane furanodiptherineglycosidae exhibits anticancer activity through induction of mitochondrial-mediated apoptosis by triggering reactive oxygen species and autophagy [6]. Phenolic compounds have genoprotective and antioxidant effects on cancer cells [8].…”

Section: Phytocomponents and Its Mechanism Of Action Against Cancer Cmentioning

confidence: 99%

“…New clerodane-furano-diterpene-glycoside obtained as an aqueous-alcoholic extract through bioassay-guided fractionation exhibited significant cytotoxic effect and induced apoptosis in human lung carcinoma (A549), prostate (PC-3), SF-269(CNS), melanoma (MDA-MB-435), colon cancer (HCT-116) and breast cancer (MCF-7) cell lines. It was observed the induction of apoptosis was Reactive oxygen species-mediated through mitochondria by activation of the caspase pathway [6]. Singh B et al identified phenolic compounds from a fungal extract of endophytic fungus Cladosporium velox TN-9S isolated from the stem of Tc.…”

Section: Anti-carcinogenic Effect Of Tc Phytocomponentsmentioning

confidence: 99%

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Effect of Tinospora cordifolia-Derived Phytocomponents on Cancer: A Systematic Review

et al. 2019

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The major cancer therapeutic modalities include surgery, chemotherapy and radiotherapy. Although these treatment regimens have played a significant role in effectively inhibiting cancer, their associated morbidity reduces the overall quality of life. Thus, researchers are striving to identify any alternate therapeutic approach capable of inhibiting cancer without eliciting the added morbidity. Among the alternate cancer therapeutics being researched, much importance is being given to the use of plants due to the presence of a wide variety of anti-carcinogenic compounds. Tinospora cordifolia (Tc) is one such plant and has shown to exhibit anti-carcinogenic properties. The present review aimed to systematically analyze published data on the effect of Tinospora cordifolia-derived phytocomponents on cancer. PubMed, Scopus, Web of Science, Embase and Cochrane library were searched using the keywords Tinospora cordifolia; anticancer; phytocomponents until March 20, 2019. In vivo and in vitro original studies in the English language were included. Of the 342 articles identified, only 25 articles met the selection criteria and were included in the review. Significant anti-carcinogenic properties were exhibited by Tinospora cordifolia-derived phytocompounds including palmative, berberine, new clerodane furanoditerene glycoside, arabinogalactan, phenolic compounds and epoxy cleodane diterpene. No significant side effects have been elicited with its use. Based on the data from the included studies, Tinospora cordifolia could be a natural therapeutic agent for cancer, provided its anti-carcinogenic properties can be elicited consistently at a large scale in clinical trials.

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Fucoxanthin prevents cell growth and induces apoptosis in endometrial cancer HEC‐1A cells by the inhibition of the PI3K/Akt/mTOR pathway

Sun

Yang

et al. 2022

J Biochem & Molecular Tox

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Endometrial cancer is the major type of gynecological cancer and ranks as the sixth most common cancer in women. Endometrial cancer usually is diagnosed in an advanced stage, complicating the treatments in many cases. The present research was focused on unveiling the in vitro anticancer role of fucoxanthin against the endometrial cancer HEC‐1A cells by inhibiting the phosphatidylinositol‐3‐kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) signaling axis. The cytotoxicity of fucoxanthin against the endometrial cancer HEC‐1A cells was studied using the MTT test. The level of reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP) status, and apoptotic cell death in the 7.5 and 10 µM administered HEC‐1A cells were assayed using fluorescent staining techniques. The messenger RNA expression was analyzed using RT‐PCR for PI3K/Akt/mTOR signaling molecules, proapoptotic (Bax and caspase‐3) antiapoptotic (cyclin D1 and Bcl‐2) genes, and inflammatory markers like tumour necrosis factor α (TNFα), nuclear factor kappa B (NF‐κB), Cox‐2, and interleukin (IL)‐6. The cell viability assay proved that fucoxanthin effectively prevented HEC‐1A cell viability, where the IC50 was 7.5 µM. Fucoxanthin at 7.5 and 10 µM remarkably improved ROS production and apoptosis and decreased the MMP in HEC‐1A cells. The fucoxanthin effectively inhibited the PI3K/Akt/mTOR cascade along with the expression of TNF‐α, NF‐κB, Cox‐2, and IL‐6 and antiapoptotic genes cyclin D1 and Bcl‐2 in the HEC‐1A cells. Fucoxanthin treatment also enhanced the Bax and caspase‐3 expressions in the HEC‐1A cells. Our results from this work unveiled that fucoxanthin triggered growth inhibition and apoptosis in endometrial cancer HEC‐1A cells. Besides, fucoxanthin inhibited the PI3K/Akt/mTOR cascade and improved apoptotic marker expressions in the HEC‐1A cells.

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A new clerodane furano diterpene glycoside from Tinospora cordifolia triggers autophagy and apoptosis in HCT-116 colon cancer cells

Cited by 32 publications

References 42 publications

Tinospora cordifolia preserves pancreatic beta cells and enhances glucose uptake in adipocytes to regulate glucose metabolism in diabetic rats

Tinospora cordifolia preserves pancreatic beta cells and enhances glucose uptake in adipocytes to regulate glucose metabolism in diabetic rats

Effect of Tinospora cordifolia-Derived Phytocomponents on Cancer: A Systematic Review

Fucoxanthin prevents cell growth and induces apoptosis in endometrial cancer HEC‐1A cells by the inhibition of the PI3K/Akt/mTOR pathway

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