Murine model of hepatic breast cancer

Abstract

Background and aimsBreast cancer is the most common cancer in women and the second leading cause of cancer-related deaths in this population. Breast cancer related deaths have declined due to screening and adjuvant therapies, yet a driving clinical need exists to better understand the cause of the deadliest aspect of breast cancer, metastatic disease. Breast cancer metastasizes to several distant organs, the liver being the third most common site. To date, very few murine models of hepatic breast cancer exist.MethodsIn this study, a novel murine model of liver breast cancer using the MDA-MB-231 cell line is introduced as an experimental (preclinical) model.ResultsHistological typing revealed consistent hepatic breast cancer tumor foci. Common features of the murine model were vascular invasion, lung metastasis and peritoneal seeding.ConclusionsThe novel murine model of hepatic breast cancer established in this study provides a tool to be used to investigate mechanisms of hepatic metastasis and to test potential therapeutic interventions.

State of the Art Modelling of the Breast Cancer Metastatic Microenvironment: Where Are We?

“…In another attempt to develop a model of liver metastasis, Rikhi et al, [ 108 ] performed direct injection of liver cells into the frontal liver lobe of a FRG™ KO [ Fah(-/-) Rag2(-/-)Il2r g (-/-)]) NOD mouse which has been shown previously to allow successful colonisation of human hepatocytes [ 109 ]. Co-injection with human hepatocytes had no influence of tumour growth so in this model MDA-MB-231 were injected alone.…”