“…The adipogenic potential of hASCs tends to decline with consecutive passages under standard culture conditions (Li et al, 2017 ). To induce this process in vitro , we used low passage (up to 6) hASCs with an optimized drug cocktail (Gojanovich et al, 2016 ), based on a comparison of previously used formulations (Gimble and Guilak, 2003 ; Lee et al, 2004 ; Rodriguez et al, 2004 ; Dicker et al, 2005 ; Bunnell et al, 2008 ; Zhu et al, 2008 ; da Silva Meirelles and Covas, 2011 ; Kim et al, 2016 ). These include DMEM (high or low glucose), 10% v/v FBS, and variable concentrations of Insulin, Dexamethasone, Indomethacin, or Rosiglitazone, with or without 0.5 mM IBMX.…”