Effects of Different Suture Materials on Tissue Healing

Selvi, Fırat; Çakarer, Sırmahan; Can, Taylan; Topcu, Serpil İrem Kirli; Palancıoğlu, Alen; Keskin, Başak; Bılgıç, Bilge; Yaltırık, Mehmet; Keskin, Cengizhan

doi:10.17096/jiufd.79438

Cited by 33 publications

(24 citation statements)

References 16 publications

(28 reference statements)

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“…On the other side, MSCs can control pro-inflammatory cytokines release, particularly IFN-g, IL-2, TNF-a. The release of IL 10 and TGF-b of MSCs post-TNF-α exposure may suppress inflammation, thus accelerate the wound healing process [16].…”

Section: Introductionmentioning

confidence: 99%

Hypoxia-Mesenchymal Stem Cells Inhibit Intra-Peritoneal Adhesions Formation by Upregulation of the IL-10 Expression

Muhar

Putra

Warli

et al. 2019

Open Access Maced J Med Sci

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BACKGROUND: Intra-peritoneal adhesions (IPAs) common occurre in post abdominal surgical. Athough many methods have been developed for controlling IPAs, including mesenchymal stem cells (MSCs) application, however, there is none completely preventing in due to the mesothelial structure may promote the prolonged inflammations leading. Nevertheless hypoxia-MSCs (H-MSCs) have more potent in controlling the inflammation than normoxia-MSCs (N-MSCs) by releasing several anti-inflamation particularly IL-10, however the H-MSCs application to inhibit IPAs remain unclear. AIM: The aim of this study was to investigate the effectiveness of H-MSCs in preventing the AIPs event by releasing IL-10 on the ileum abrasion sutured omental patch as the animal model of peritoneal adhesion. METHODS: Using 24 IPAs animal model were randomly divided into 4 groups: Sham (Sh), Control (C), H-MSCs at high dose (T1) and H-MSCs at low dose (T2). H-MSCs were incubated under hypoxic conditions (5% O2), 37°C and 5% CO2 for 24 hours. The expression level of IL-10 was performed using RT-PCR analysis. The macroscopic appearance of IPAs was evaluated using Nair’s scale base on the absence/presence of adhesion, whereas the microscopic by Zuhlke’s scale at Hematoxylin and eosin (H&E) staining. RESULTS: This study showed a significanly increase in IL-10 expression (p < 0.05) at all T groups. In line with this, we also found a significant difference in IPAs between T groups and Control as well as a Sham (p < 0.05) either in the macroscopic or microscopic analysis. CONCLUSION: H-MSCs has a robust ability in inhibiting severe IPAs characterized by the decreased of adhesion formation and the enhanced expression of IL-10.

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Section: Introductionmentioning

confidence: 99%

Hypoxia-Mesenchymal Stem Cells Inhibit Intra-Peritoneal Adhesions Formation by Upregulation of the IL-10 Expression

Muhar

Putra

Warli

et al. 2019

Open Access Maced J Med Sci

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“…Sutures play a role in maintaining tissue approximation, thus inducing the wound to achieve a sufficient level of tensional strength and thereby preventing wound dehiscence. Correct closure and stabilization of surgical wound margins may have a significant effect on the successful outcomes of surgery [3,4].…”

Section: Introductionmentioning

confidence: 99%

Biomechanical Properties and Biocompatibility of a Non-Absorbable Elastic Thread

Choi¹,

Kang²,

Choi³

et al. 2019

JFB

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To date, extensive studies have been conducted to assess diverse types of sutures. But there is a paucity of data regarding biomechanical properties of commonly used suture materials. In the current experiment, we compared biomechanical properties and biocompatibility, such as tensile strength and elongation, the degree of bovine serum albumin (BSA) release, in vitro cytotoxicity and ex vivo frictional properties, between a non-absorbable elastic thread (NAT; HansBiomed Co. Ltd., Seoul, Korea) (NAT-R: NAT with a rough surface, NAT-S: NAT with a smooth surface) and the Elasticum® (Korpo SRL, Genova, Italy). The degree of tensile strength and elongation of Si threads was significantly higher in both the NAT-R and -S as compared with the Elasticum® (p < 0.05). Moreover, the degree of tensile strength and elongation of PET threads was significantly lower in both NAT-R and -S as compared with the Elasticum® (p < 0.05). Furthermore, the degree of tensile strength and elongation of braided Si/PET threads was significantly lower in NAT-S as compared with NAT-R and Elasticum® (p < 0.05). The degree of BSA release was significantly higher in the NAT-R as compared with Elasticum® and NAT-S throughout a 2-h period in the descending order (p < 0.05). The degree of cell viability was significantly higher in both NAT-R and -S as compared with Elasticum® (p < 0.05). The degree of coefficient of friction as well as the frictional force and strength was significantly higher in NAT-R as compared with NAT-S and Elasticum® (p < 0.05). NAT had a higher degree of biomechanical properties and biocompatibility as compared with Elasticum®. But further experimental and clinical studies are warranted to compare the efficacy, safety, and potential role as a carrier for drug delivery between NAT and Elasticum®.

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“…Polyglactin, because it is absorbable and multifilamentous, induces a greater inflammatory reaction than polypropylene, as it is a monofilament material. 15,16 However, a greater degree of fibrosis was expected that in the group submitted only to fistulotomy and curettage, since the wound left for healing by second intention would have a greater inflammatory response and consequently greater fibrosis, 17 which did not occur; in that group, the fibrosis observed was equivalent to that in polypropylene-treated animals and significantly lower than in the polyglactin group.…”

Section: Discussionmentioning

confidence: 96%

Evaluation of polypropylene and polyglactin sutures in primary sphincteroplasty for the treatment of anal fistula in rats

Conde

Santos

Dourado

et al. 2019

Journal of Coloproctology

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Rationale Fistulotomy followed by primary sphincteroplasty is one of the therapeutic options in transsphincteric fistulae; however, it was not known which suture would present a better result. Objective To compare polypropylene and polyglactin sutures in primary sphincteroplasty in rats subjected to fistulotomy. Method Thirty Wistar rats were subjected to peritoneal anesthesia with ketamine and xylazine, followed by transfixation of the anal sphincter with steel thread, which remained for 30 days to develop the anal fistula. After this period, the steel thread was removed and four groups were formed: A – Control ( n = 5), without treatment; B – Fistulotomy ( n = 5), performed fistulotomy only; C – Polypropylene ( n = 10), in which fistulotomy was performed followed by primary sphincteroplasty with polypropylene suture; D – Polyglactin ( n = 10), in which fistulotomy was performed followed by primary sphincteroplasty with polyglactin suture; after 30 days the animals were anesthetized again and submitted to euthanasia by deepening the anesthetic plane to remove the specimens, analyzing fistula closure, muscle fiber distance, and inflammatory process. Results The fistula persisted in all animals of the control group and in none of the other groups; the distances between the muscle fibers were 1620 μm, 4665 μm, and 2520 μm, respectively in Groups B, C, and D ( p = 0.067); in relation to fibrosis, the means were 2.4, 2.8, and 3.6, respectively in Groups B, C, and D, showing greater fibrosis in the latter group ( p = 0.041). Conclusion There was no persistence of the fistula in any of the treated animals; there was no difference in the distance between the muscle fibers between the groups subjected to primary sphincteroplasty with polypropylene or polyglactin, or between these groups and the one treated only by fistulotomy. There was greater fibrosis in animals treated with primary sphincteroplasty with polyglactin.

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Effects of Different Suture Materials on Tissue Healing

Cited by 33 publications

References 16 publications

Hypoxia-Mesenchymal Stem Cells Inhibit Intra-Peritoneal Adhesions Formation by Upregulation of the IL-10 Expression

Hypoxia-Mesenchymal Stem Cells Inhibit Intra-Peritoneal Adhesions Formation by Upregulation of the IL-10 Expression

Biomechanical Properties and Biocompatibility of a Non-Absorbable Elastic Thread

Evaluation of polypropylene and polyglactin sutures in primary sphincteroplasty for the treatment of anal fistula in rats

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