Detection of Zika Virus in April 2013 Patient Samples, Rio de Janeiro, Brazil

Passos, Sônia Regina Lambert; Santos, Maria Angélica Borges dos; Cerbino-Neto, José; Buonora, Sibelle Nogueira; Souza, Thiago Moreno L.; Oliveira, Raquel de Vasconcellos Carvalhaes de; Vizzoni, AG; Barbosa-Lima, Giselle; Vieira, Yasmine Rangel; Lima, Marcondes Silva de; Hökerberg, Yara Hahr Marques

doi:10.3201/eid2312.171375

Cited by 27 publications

(21 citation statements)

References 10 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The virus was likely brought to Brazil by a traveler from French Polynesia after a stop at Easter Island ( 10 – 12 ). A recent article from Passos et al performed a retrospective blood blank analysis on 210 samples collected from patients during a DENV-4 outbreak that occurred in early 2013 ( 13 ). Of these samples, 10% showed a singly positive qRT-PCR result for ZIKV, and only 2% demonstrated consistently positive results across triplicate samples.…”

Section: History Of Virus Emergencementioning

confidence: 99%

Evolution of Two Major Zika Virus Lineages: Implications for Pathology, Immune Response, and Vaccine Development

et al. 2018

View full text Add to dashboard Cite

Zika virus (ZIKV) became a public health emergency of global concern in 2015 due to its rapid expansion from French Polynesia to Brazil, spreading quickly throughout the Americas. Its unexpected correlation to neurological impairments and defects, now known as congenital Zika syndrome, brought on an urgency to characterize the pathology and develop safe, effective vaccines. ZIKV genetic analyses have identified two major lineages, Asian and African, which have undergone substantial changes during the past 50 years. Although ZIKV infections have been circulating throughout Africa and Asia for the later part of the 20th century, the symptoms were mild and not associated with serious pathology until now. ZIKV evolution also took the form of novel modes of transmission, including maternal–fetal transmission, sexual transmission, and transmission through the eye. The African and Asian lineages have demonstrated differential pathogenesis and molecular responses in vitro and in vivo. The limited number of human infections prior to the 21st century restricted ZIKV research to in vitro studies, but current animal studies utilize mice deficient in type I interferon (IFN) signaling in order to invoke enhanced viral pathogenesis. This review examines ZIKV strain differences from an evolutionary perspective, discussing how these differentially impact pathogenesis via host immune responses that modulate IFN signaling, and how these differential effects dictate the future of ZIKV vaccine candidates.

show abstract

Section: History Of Virus Emergencementioning

confidence: 99%

Evolution of Two Major Zika Virus Lineages: Implications for Pathology, Immune Response, and Vaccine Development

et al. 2018

View full text Add to dashboard Cite

show abstract

“…All nondengue cases were examined by RT‐PCR for Zika, and three cases were detected. (Passos et al, 2017). Inter‐agreement rates did not vary according to a confirmed diagnosis of dengue based on reference tests.…”

Section: Resultsmentioning

confidence: 98%

“…At the time of data collection for this study, there was evidence of co‐circulation of other arboviruses such as Zika (Passos et al, 2017). The magnitude of this co‐circulation probably did not affect results.…”

Section: Discussionmentioning

confidence: 97%

Pitfalls in acute febrile illness diagnosis: Interobserver agreement of signs and symptoms during a dengue outbreak

Buonora

Passos

Daumas

et al. 2020

Journal of Clinical Nursing

View full text Add to dashboard Cite

Aims and objectives To compare and evaluate interobserver (nurses and physicians) agreement for dengue clinical signs and symptoms, including the World Health Organization diagnostic algorithm. Background Agreement of clinical history defines the capacity of the examiner to measure a given clinical parameter in a reproducible and consistent manner, which is prerequisite for diagnosis validity. Nurses play a major role in the triage and care of dengue patients in many countries. Study design This is a sub‐study on interobserver agreement performed as part of a cross‐sectional diagnostic accuracy study for acute febrile illness (AFI) using the checklist STARD. Methods A previously validated semi‐structured sign and symptom standardised questionnaire for AFI was independently administered to 374 patients by physician and nurse pairs. The interobserver agreement was estimated using kappa statistics. Results For a set of 27 signs and symptoms, we found six interobserver discrepancies (examiner detected red eyes, lethargy, exanthema, dyspnoea, bleeding and myalgia) as identified by regular and moderate kappa indexes. Four signs (patient observed red eyes, cough, diarrhoea and vomiting) and one symptom (earache) had near‐perfect agreement. Most signs and symptoms showed substantial agreement. The WHO (Dengue guidelines for diagnosis, treatment, prevention and control: new edition, World Health Organization, 2009) clinical criteria for dengue comprise a group of symptoms known as “pains and aches.” Interobserver agreement for abdominal pain, retro‐orbital pain and arthralgia exceed that found for headache and myalgia. Conclusions During a dengue outbreak, the interobserver agreement for most of the signs and symptoms used to assess AFI was substantial. Relevance to clinical practice This result suggests good potential applicability of the tool by health professionals following training. A well‐trained health professional is qualified to apply the standardised questionnaire to evaluate suspected dengue cases during outbreaks.

show abstract

“…Muscular pain is a recurrent symptom associated with ZIKV infection, affecting up to 65% of patients in some outbreaks (26, 27). Skeletal muscle is a widespread body tissue comprised by mature fibers and myogenic precursor cells that are responsible for muscle development and repair (28).…”

Section: Discussionmentioning

confidence: 99%

Zika virus replicates in skeletal muscle contributing to peripheral viral amplification prior to reach neural tissue

Gavino-Leopoldino

et al. 2020

Preprint

View full text Add to dashboard Cite

Zika virus (ZIKV) infections are still a worldwide concern due to the severity of neurological outcomes. ZIKV neurotropism is well characterized, but peripheral tissue could be sites of viral amplification, contributing to endothelial-barrier crossing and access to peripheral nerves. During acute and late phases of infection, ZIKV can be detected in several body fluids, eyes, testis and vagina. However, the importance of initial replication sites for the establishment of infection and viral spread remain unknown. Here we demonstrated that ZIKV replicates primarily in human muscle precursor cells, resulting in cell death and inhibition of myogenesis. ZIKV also replicates in fetal muscle after maternal transmission and in infected neonate mice, inducing lesions and inflammation. Muscle was an important site of viral amplification, sustaining higher peripheral viral loads than liver and spleen. In addition, ZIKV showed rapid and sustained replication kinetics in muscle even before replication in the neural tissues, persisting until 16 days post infection. Our results highlight the importance of muscle in ZIKV pathogenesis as a peripheral site of viral amplification which may contribute to ZIKV reaching neural structures.Author SummaryZika Virus (ZIKV) neurotropism and its deleterious effects on central nervous system have been well characterized. But, investigations of the initial replication sites for the establishment of infection and viral spread to neural tissues remain under explored. Here we demonstrated that ZIKV replicates primarily in human skeletal muscle precursor cells, resulting in cell death and disrupted myogenesis. ZIKV also replicates in muscle of fetus and neonate mice inducing muscle damage and inflammation. Muscle replication occurs before amplification in peripheral nerves and brain, contributing to the increase of peripheral ZIKV load and dissemination. In addition, ZIKV RNA still been detected in skeletal muscle at late stages of infection. Overall, our findings showed that skeletal muscle is involved in ZIKV pathogenesis, contributing to a broader understanding of ZIKV infection. Thus, opens new aspects in the investigation of the long-term consequence of early infection.

show abstract

Detection of Zika Virus in April 2013 Patient Samples, Rio de Janeiro, Brazil

Cited by 27 publications

References 10 publications

Evolution of Two Major Zika Virus Lineages: Implications for Pathology, Immune Response, and Vaccine Development

Evolution of Two Major Zika Virus Lineages: Implications for Pathology, Immune Response, and Vaccine Development

Pitfalls in acute febrile illness diagnosis: Interobserver agreement of signs and symptoms during a dengue outbreak

Zika virus replicates in skeletal muscle contributing to peripheral viral amplification prior to reach neural tissue

Contact Info

Product

Resources

About