A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis

Abstract

The etiology of autoinflammation in systemic juvenile idiopathic arthritis is unclear. Cepika et al. use integrated analysis of multidimensional blood stimulation data, applied to patients while off treatment and in complete remission, to reveal underlying cellular and molecular mechanisms that might predispose to disease.

Precision medicine using different biological DMARDs based on characteristic phenotypes of peripheral T helper cells in psoriatic arthritis

“…For instance, whole-blood transcriptomics, serum proteomics and immune phenotyping was used in an attempt to quantitatively measure molecular remission in patients with rheumatoid arthritis [2]. In two other studies, transcriptome analysis, among other techniques, was harnessed to uncover the molecular networks underlying disease like juvenile idiopathic arthritis and juvenile systemic lupus erythematosus [3,4]. Finally, the clinical applicability of cell phenotyping in defining treatment strategies for patients with psoriatic arthritis is enticing [5].…”

Development and Characterization of a Fixed Repertoire of Blood Transcriptome Modules Based on Co-expression Patterns Across Immunological States

“…But studies have also investigated changes in transcriptome abundance in isolated leukocyte populations as well as in single cells [5, 6]. Global changes in transcript abundance can also be observed upon stimulation of the cells in vitro with host or environmental derived immunogenic factors, such as pathogen-associated molecular pattern, antigenic peptides, as well as pro or anti-inflammatory cytokines or chemokines [7, 8].…”

ATP triggers a robust intracellular [Ca2+]‐mediated signalling pathway in human synovial fibroblasts

“…Since some isoforms of adenylyl cyclase are Ca 2+ dependent it is possible that both of the agonists described in this paper could reduce fibrosis. Examination of this possibility and a number of others will be guided and accelerated by recent papers regarding the articular joint transciptome and proteome (Bhattacharjee et al., ; Cepika et al., )‐related systems biology methods and approaches (Distler et al., ; Groenendyk et al., ; Hui et al., ; Ogawa et al., ; Aitchison and Rout, ) and emerging knowledge of the cytokine profile in these joints under baseline conditions and as documented arthritis phenotypes are initiated and progress (Armaka et al., ).…”