Detection of membrane-bound and soluble antigens by magnetic levitation

Andersen, Mikkel Schou; Howard, Emily; Lu, Shulin; Richard, M Schwartz; Gregory, Mark; Ogembo, Javier Gordon; Mazor, Ofer; Gorelik, Pavel; Shapiro, Nathan I.; Sharda, Anish V.; Ghiran, Ionita

doi:10.1039/c7lc00402h

Cited by 21 publications

(37 citation statements)

References 30 publications

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“…[63][64][65][66][67][68][69][70] Ghiran, Tasoglu, and colleagues have developed ar ange of new techniques (particularly in the combined use of MagLev and smartphones) and applications in medical analysis (cells and assays). [71][72][73][74][75][76][77][78] TheY ildiz group has developed am ethod to carry out scaffold-free cell cultures. [6,79] Zhao and colleagues have made important advances in developing the technology base (particularly asingle-ring MagLev system [80] )and applications in materials science (particularly for polymeric materials).…”

Section: Objective and Focus Of The Reviewmentioning

confidence: 99%

“…[89] Grzybowski has developed potentially important methods to carry out magnetic tweezing and self-assembly of diamagnetic colloids in paramagnetic solutions. [90][91][92] Thew ork of these groups is summarized in their papers and in separate reviews; [6,7,69,72,76,[87][88][89]92] their work, and these summaries and analyses,a re described and referenced in appropriate sections of this Review.…”

Section: Objective and Focus Of The Reviewmentioning

confidence: 99%

“…When the antigen is present on the surface of acell, abead bearing an antibody against the antigen binds to the cell and forms an aggregate,w hich has ad ensity different from both the bead and the cell, and thus,levitates at adifferent height from the non-aggregated bead or cell in aMagLev device. [72] Then umber of these aggregates correlated with-and thus, could be used to quantify-the abundance of the antigens of interest. This assay could sensitively detect an umber of surface-bound antigens found in blood, including T-cell antigen CD3, eosinophil antigen Siglec-8, red blood cell antigens CD35 and RhD,and red blood cell-bound Epstein-Barr viral particles.F or example,F igure 13 Gs hows the sensitive detection of eosinophil granulocytes,at ype of cells only making up 2-3 %ofall nucleated cells in normal blood, using anti-Siglec-8 antibody-coated beads.…”

Section: Metal-amplified Density Assays For Antigens and Antibodiesmentioning

confidence: 99%

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Magnetic Levitation in Chemistry, Materials Science, and Biochemistry

et al. 2020

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All matter has density. The recorded uses of density to characterize matter date back to as early as ca. 250 BC, when Archimedes was believed to have solved “The Puzzle of The King's Crown” using density.[1] Today, measurements of density are used to separate and characterize a range of materials (including cells and organisms), and their chemical and/or physical changes in time and space. This Review describes a density‐based technique—magnetic levitation (which we call “MagLev” for simplicity)—developed and used to solve problems in the fields of chemistry, materials science, and biochemistry. MagLev has two principal characteristics—simplicity, and applicability to a wide range of materials—that make it useful for a number of applications (for example, characterization of materials, quality control of manufactured plastic parts, self‐assembly of objects in 3D, separation of different types of biological cells, and bioanalyses). Its simplicity and breadth of applications also enable its use in low‐resource settings (for example—in economically developing regions—in evaluating water/food quality, and in diagnosing disease).

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Section: Objective and Focus Of The Reviewmentioning

confidence: 99%

Section: Objective and Focus Of The Reviewmentioning

confidence: 99%

Section: Metal-amplified Density Assays For Antigens and Antibodiesmentioning

confidence: 99%

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Magnetic Levitation in Chemistry, Materials Science, and Biochemistry

et al. 2020

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“…Andersen et al . detected cell-membrane-bound and soluble antigens in magnetically levitated cells 37 . Engineered as a compact and portable unit, magnetic levitation was used to distinguish sickle RBCs from normal cells 38 .…”

Section: Introductionmentioning

confidence: 98%

Shape oscillations of single blood drops: applications to human blood and sickle cell disease

Hosseinzadeh

Brugnara

Holt

2018

Sci Rep

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Sickle cell disease (SCD) is an inherited blood disorder associated with severe anemia, vessel occlusion, poor oxygen transport and organ failure. The presence of stiff and often sickle-shaped red blood cells is the hallmark of SCD and is believed to contribute to impaired blood rheology and organ damage. Most existing measurement techniques of blood and red blood cell physical properties require sample contact and/or large sample volume, which is problematic for pediatric patients. Acoustic levitation allows rheological measurements in a single drop of blood, simultaneously eliminating the need for both contact containment and manipulation of samples. The technique shows that the shape oscillation of blood drops is able to assess blood viscosity in normal and SCD blood and demonstrates an abnormally increased viscosity in SCD when compared with normal controls. Furthermore, the technique is sensitive enough to detect viscosity changes induced by hydroxyurea treatment, and their dependence on the total fetal hemoglobin content of the sample. Thus this technique may hold promise as a monitoring tool for assessing changes in blood rheology in sickle cell and other hematological diseases.

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“…Magnetic levitation is a relatively new technique that uses a uniform magnetic field to measure and separate cells based on their density, and magnetic properties. (9)(10)(11)(12). While the magnetic levitation technology offers a variety of opportunities for functional interrogation of cells, for this specific study, we use it as a tool to: a) isolate leukocytes from circulating red blood cells (RBCs), b) visualize leukocytes, and c) measure size, shape, density and paramagnetic property parameters of leukocytes.…”

Section: Introductionmentioning

confidence: 99%