‘ Haemophilus quentini ’ in the urethra of men complaining of urethritis symptoms

Horie, Kengo; Ito, Shin; Hatazaki, Kyoko; Yasuda, Mitsuru; Nakano, Masahiro; Kawakami, Kyojiro; Fujita, Yasunori; Ito, Masafumi; Ezaki, Takayuki; Deguchi, Takashi

doi:10.1016/j.jiac.2017.08.007

Cited by 8 publications

(6 citation statements)

References 14 publications

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“…Other bacterial pathogens have been implicated as potential causes of clinical urethritis, either in clustered case series or as sporadic cases such as Haemophilus influenzae and Haemophilus parainfluenzae (719)(720)(721)(722)(723). Haemophilus was identified in 12.6% of cases among 413 men (mostly MSM reporting insertive oral sex) ( 724), and high rates of azithromycin resistance (39.5%) were identified among Haemophilus urethritis patients (725).…”

Section: Etiologymentioning

confidence: 99%

Sexually Transmitted Infections Treatment Guidelines, 2021

Workowski¹,

Bachmann²,

Chan³

et al. 2021

MMWR Recomm. Rep.

1,570

2,621

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These guidelines for the treatment of persons who have or are at risk for sexually transmitted infections (STIs) were updated by CDC after consultation with professionals knowledgeable in the field of STIs who met in Atlanta, Georgia, June 11-14, 2019. The information in this report updates the 2015 guidelines. These guidelines discuss 1) updated recommendations for treatment of Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis; 2) addition of metronidazole to the recommended treatment regimen for pelvic inflammatory disease; 3) alternative treatment options for bacterial vaginosis; 4) management of Mycoplasma genitalium; 5) human papillomavirus vaccine recommendations and counseling messages; 6) expanded risk factors for syphilis testing among pregnant women; 7) one-time testing for hepatitis C infection; 8) evaluation of men who have sex with men after sexual assault; and 9) two-step testing for serologic diagnosis of genital herpes simplex virus. Physicians and other health care providers can use these guidelines to assist in prevention and treatment of STIs. BOX 1. The Five P's approach for health care providers obtaining sexual histories: partners, practices, protection from sexually transmitted infections, past history of sexually transmitted infections, and pregnancy intention Additional information about external condoms is available at https://www.cdc.gov/condomeffectiveness. Internal CondomsCondoms for internal vaginal use, also known as female condoms, are available worldwide (e.g., the FC2 Female Condom, Reddy condom, Cupid female condom, and Woman's condom) (31,32). Use of internal condoms can provide protection from acquisition and transmission of STIs, although data are limited. Internal condoms are more costly compared with external condoms; however, they offer the advantage of being controlled by the receptive partner as an STI and HIV prevention method, and the newer versions might be acceptable to all persons. Although the internal condom also has been used during receptive anal intercourse, efficacy associated with this practice remains unknown (33). Additional information about the internal condom is available at http://www.ashasexualhealth. org/pdfs/Male_and_Female_Condoms.pdf. Cervical DiaphragmsIn observational studies, diaphragm use has been demonstrated to protect against cervical gonorrhea, chlamydia, and trichomoniasis (34). However, a trial examining the effect of a diaphragm plus lubricant on HIV acquisition among women in Africa reported no additional protective effect when compared with the use of male condoms alone. Likewise, no difference by study arm in the rate of acquisition of chlamydia, gonorrhea, or herpes occurred (35,36). Diaphragms should not be relied on as the sole source of protection against HIV and other STIs.

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Section: Etiologymentioning

confidence: 99%

Sexually Transmitted Infections Treatment Guidelines, 2021

Workowski¹,

Bachmann²,

Chan³

et al. 2021

MMWR Recomm. Rep.

1,570

2,621

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“…In the current study, differential abundance testing within voided urine samples did identify two species that differed by sex: Haemophilus (over-represented in males) and Schaalia canis (over-represented in females). Haemophilus has been well-documented in the male urethra, with various strains of Haemophilus shown to associate with urethritis in men [ 55 – 58 ], and Schaalia canis , formerly known as Actinomyces canis [ 59 , 60 ], has been previously isolated from the canine vagina [ 61 ]. These findings support the notion that urethro-genital microbes can contaminate the UM of voided canine urine samples and contribute to differences between sexes.…”

Section: Discussionmentioning

confidence: 99%

The impact of urine collection method on canine urinary microbiota detection: a cross-sectional study

et al. 2023

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Background The urinary tract harbors unique microbial communities that play important roles in urogenital health and disease. Dogs naturally suffer from several of the same urological disorders as humans (e.g., urinary tract infections, neoplasia, urolithiasis) and represent a valuable translational model for studying the role of urinary microbiota in various disease states. Urine collection technique represents a critical component of urinary microbiota research study design. However, the impact of collection method on the characterization of the canine urinary microbiota remains unknown. Therefore, the objective of this study was to determine whether urine collection technique alters the microbial populations detected in canine urine samples. Urine was collected from asymptomatic dogs by both cystocentesis and midstream voiding. Microbial DNA was isolated from each sample and submitted for amplicon sequencing of the V4 region of the bacterial 16 S rRNA gene, followed by analyses to compare microbial diversity and composition between urine collection techniques. Results Samples collected via midstream voiding exhibited significantly higher sequence read counts (P = .036) and observed richness (P = .0024) than cystocentesis urine. Bray Curtis and Unweighted UniFrac measures of beta diversity showed distinct differences in microbial composition by collection method (P = .0050, R2 = 0.06 and P = .010, R2 = 0.07, respectively). Seven taxa were identified as differentially abundant between groups. Pasteurellaceae, Haemophilus, Friedmanniella, two variants of Streptococcus, and Fusobacterium were over-represented in voided urine, while a greater abundance of Burkholderia-Caballeronia-Paraburkholderia characterized cystocentesis samples. Analyses were performed at five thresholds for minimum sequence depth and using three data normalization strategies to validate results; patterns of alpha and beta diversity remained consistent regardless of minimum read count requirements or normalization method. Conclusion Microbial composition differs in canine urine samples collected via cystocentesis as compared to those collected via midstream voiding. Future researchers should select a single urine collection method based on the biological question of interest when designing canine urinary microbiota studies. Additionally, the authors suggest caution when interpreting results across studies that did not utilize identical urine collection methods.

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“…The recognition that some NTHi biotype IV isolates were commonly recovered from the genitourinary (GU) tract was first reported in Canada in the late 1970s (12,13). Subsequently, many of these isolates were confirmed to be associated with urogenital and neonatal infections, and several groups postulated that these strains had a predilection for the female genital tract (8)(9)(10)(11)(14)(15)(16)(17). Using electrophoretic typing, Quentin et al demonstrated in 1990 that a group of NTHi biotype IV isolates, predominantly from patients with obstetric infections or serious neonatal disease, was divergent enough from H. influenzae sensu stricto that it definitively represented a novel species of Haemophilus (10).…”

mentioning

confidence: 99%

“…These organisms were then called "H. influenzae cryptic genospecies" and, later, Haemophilus quentini, a name that has never been formally proposed (8)(9)(10)(11). More recently, a number of reports have described H. quentini strains isolated from the urinary tracts of adult men and noted their association with urethritis in this group (16,17). Several groups have explored the role of particular adhesins that may allow for enhanced adherence of H. quentini in the urogenital niche (14,(18)(19)(20)(21)(22).…”

mentioning

confidence: 99%

“…These three species differ from other Haemophilus species in their requirement for both X and V growth factors, but there is a high degree of similarity between them with respect to morphology and both phenotypic and genetic characteristics, as well as the possibility of interspecies recombination, which may further complicate laboratory identification (2,23). Given that H. haemolyticus is typically considered a commensal organism with limited pathogenicity and is rarely implicated in invasive disease (2,24,25), while NTHi has emerged as a known significant pathogen and H. quentini is increasingly recognized as a neonatal, maternal, and urogenital pathogen (8,9,11,(14)(15)(16)(17), accurate identification is important.…”

mentioning

confidence: 99%

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Identification and Characterization of “ Haemophilus quentini ” Strains Causing Invasive Disease in Ontario, Canada (2016 to 2018)

et al. 2019

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Haemophilus influenzae is a well-established human pathogen capable of causing a range of respiratory and invasive diseases. Since the 1970s, it has been observed that a nontypeable cryptic genospecies of H. influenzae, most often biotype IV, has been associated with the genitourinary tracts of females and with invasive neonatal infections. This distinct genospecies has been provisionally named “Haemophilus quentini.” Here, we report seven cases of invasive H. quentini disease in patients from Ontario, Canada, over a 2-year period. Significantly, while most reports of invasive disease with H. quentini to date have been in neonates, we observed five cases in adults (three in women of childbearing age and two in seniors) as well as two in neonates. Identification of H. quentini is challenging and was not possible for frontline laboratories, requiring work at the reference laboratory level. We describe in detail the biochemical results, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-Tof MS) results, and PCR results with several targets, including the 16S rRNA gene and multilocus sequence typing (MLST) genes, for the seven Ontario H. quentini isolates and several controls. Our data, combined with those of other publications, support the fact that H. quentini is distinct from H. influenzae and Haemophilus haemolyticus. This organism is recognized as a pathogen of neonates, but we hypothesize that it may be underrecognized as an important pathogen in adults as well, particularly pregnant women. By sharing the detailed descriptions of these isolates, we hope to enable other laboratories to better identify H. quentini so that the true prevalence of this organism and disease can be explored.

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‘ Haemophilus quentini ’ in the urethra of men complaining of urethritis symptoms

Cited by 8 publications

References 14 publications

Sexually Transmitted Infections Treatment Guidelines, 2021

Sexually Transmitted Infections Treatment Guidelines, 2021

The impact of urine collection method on canine urinary microbiota detection: a cross-sectional study

Identification and Characterization of “ Haemophilus quentini ” Strains Causing Invasive Disease in Ontario, Canada (2016 to 2018)

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