Selective uptake of epidermal growth factor-conjugated gold nanoparticle (EGF-GNP) facilitates non-thermal plasma (NTP)-mediated cell death

Kim, Wanil; Na, Kyung-Yoon; Lee, Kyung-Ha; Lee, Hyun‐Wook; Lee, Jae Koo; Kim, Kyong‐Tai

doi:10.1038/s41598-017-11292-z

Cited by 19 publications

(18 citation statements)

References 48 publications

(46 reference statements)

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“…EGF-functionalized gold nanoparticles have been used in a study on multiple cell lines – breast, colon, lung, prostate and cervical cancer, and then subjected to non-thermal atmospheric pressure plasma (NTP) therapy. It was concluded that pre-administration of the conjugated nanoparticles resulted in substantial improvement in NTP treatment, the nanoparticles being internalized by receptor-mediated endocytosis, and subsequently leading to a selective increase in cellular apoptosis 67. Relying on the benefits of using the EGF molecule (small size, stability under physiological conditions, high-temperature denaturation, over 76 °C), it was also concluded that EGF-functionalized gold nanoparticles may yield promising results in photothermal therapy of cancer 68…”

Section: Egf Functionalized Nanoparticles – Results So Farmentioning

confidence: 99%

<p>Epidermal Growth Factor Receptor and Its Role in Pancreatic Cancer Treatment Mediated by Nanoparticles</p>

Grapa¹,

Mocan²,

Gonciar³

et al. 2019

IJN

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Pancreatic adenocarcinoma (PDAC) is a disease with a high incidence and a dreary prognosis. Its lack of symptomatology and late diagnosis contribute to the dearth and inefficiency of therapeutic schemes. Studies show that overexpressed epidermal growth factor receptor (EGFR) is a common occurrence, linking this to the progression of pancreatic cancer, although the association between its expression and the survival rate is rather controversial. EGFR-targeted therapy has not shown the results expected, leaving at hand more questions than answers; clearly, there is a need for a better understanding of the molecular pathways involved. Nanoparticles have been used in trying to improve the efficacy of antitumor treatment; thus, using EGFR’s ligand, EGF, for nanoconjugation, showed promising results in increasing the cellular uptake mechanisms and apoptosis of the targeted cells.

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Section: Egf Functionalized Nanoparticles – Results So Farmentioning

confidence: 99%

<p>Epidermal Growth Factor Receptor and Its Role in Pancreatic Cancer Treatment Mediated by Nanoparticles</p>

Grapa¹,

Mocan²,

Gonciar³

et al. 2019

IJN

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“…Previous studies also presented diffuse reflectance measurements for a cancer diagnosis [44][45][46], but they did not use nanoparticles as contrast agents. Recently, a novel strategy to treat cancer cells expressing a higher level of the EGFR used an epidermal growth factor-gold nanoparticle (EGF-GNP) conjugate complex [47]. Examination of cell levels showed that the EGF-GNP conjugated complex elicited phosphorylation of extracellular signal-regulated kinases (ERK) [47], and elevated EGFR expression, which led to internalization of the GNP into the cells.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a novel strategy to treat cancer cells expressing a higher level of the EGFR used an epidermal growth factor-gold nanoparticle (EGF-GNP) conjugate complex [47]. Examination of cell levels showed that the EGF-GNP conjugated complex elicited phosphorylation of extracellular signal-regulated kinases (ERK) [47], and elevated EGFR expression, which led to internalization of the GNP into the cells. Treatment by non-thermal plasma irradiation increased apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Treatment by non-thermal plasma irradiation increased apoptosis. Kim et al [47] concluded that non-thermal plasma irradiation coupled with EGF-GNP treatment can trigger DNA damage, which led to cell death. The present study confirms the value of GNPs as contrast agents because the reflection measurement is directed at their unique absorption properties in the NIR light region rather than their scattering properties.…”

Section: Discussionmentioning

confidence: 99%

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Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders

et al. 2020

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Diffusion reflectance spectroscopy measurements targeted with gold nanoparticles (GNPs) can identify residual cutaneous squamous cell carcinoma (SCC) in excision borders. Human SCC specimens were stained with hematoxylin and eosin to identify tumor borders, and reflected onto an unstained deparaffinized section. Diffusion reflection of three sites (normal and SCC) were measured before and after GNPs targeting. Hyperspectral imaging showed a mean of 2.5 sites with tumor per specimen and 1.2 tumor-free (p < 0.05, t-test). GNPs were detected in 25/30 tumor sites (sensitivity 83.3%, false-negative rate 16.6%) and 12/30 non-tumor sites (specificity 60%, false-positive rate 40%). This study verifies the use of nanotechnology in identifying SCC tumor margins. Diffusion reflection scanning has high sensitivity for detecting the residual tumor.

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“…In order to improve the GNPs’ specificity towards cancer cells, the addition of coating layers, such as hyaluronic acid (HA) [ 55 , 58 ], has been proposed. HA has been reported as a natural ligand to CD44 receptors overexpressed in certain types of breast cancers [ 59 ], and ligands [ 56 , 58 ], such as Epidermal Growth Factor (EGF) [ 56 , 58 , 60 ], a natural peptide ligand to Epidermal Growth Factor receptors commonly overexpressed in some tumor cells [ 61 ].…”

Section: Introductionmentioning

confidence: 99%

A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach

Costa

Ferreira-Gonçalves

Cardoso

et al. 2020

IJMS

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Breast cancer is one of the most frequently diagnosed malignancies and common causes of cancer death in women. Recent studies suggest that environmental exposures to certain chemicals, such as 7,12-Dimethylbenzanthracene (DMBA), a chemical present in tobacco, may increase the risk of developing breast cancer later in life. The first-line treatments for breast cancer (surgery, chemotherapy or a combination of both) are generally invasive and frequently associated with severe side effects and high comorbidity. Consequently, novel approaches are strongly required to find more natural-like experimental models that better reflect the tumors’ etiology, physiopathology and response to treatments, as well as to find more targeted, efficient and minimally invasive treatments. This study proposes the development and an in deep biological characterization of an experimental model using DMBA-tumor-induction in Sprague-Dawley female rats. Moreover, a photothermal therapy approach using a near-infrared laser coupled with gold nanoparticles was preliminarily assessed. The gold nanoparticles were functionalized with Epidermal Growth Factor, and their physicochemical properties and in vitro effects were characterized. DMBA proved to be a very good and selective inductor of breast cancer, with 100% incidence and inducing an average of 4.7 tumors per animal. Epigenetic analysis showed that tumors classified with worst prognosis were hypomethylated. The tumor-induced rats were then subjected to a preliminary treatment using functionalized gold nanoparticles and its activation by laser (650–900 nm). The treatment outcomes presented very promising alterations in terms of tumor histology, confirming the presence of necrosis in most of the cases. Although this study revealed encouraging results as a breast cancer therapy, it is important to define tumor eligibility and specific efficiency criteria to further assess its application in breast cancer treatment on other species.

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Selective uptake of epidermal growth factor-conjugated gold nanoparticle (EGF-GNP) facilitates non-thermal plasma (NTP)-mediated cell death

Cited by 19 publications

References 48 publications

<p>Epidermal Growth Factor Receptor and Its Role in Pancreatic Cancer Treatment Mediated by Nanoparticles</p>

<p>Epidermal Growth Factor Receptor and Its Role in Pancreatic Cancer Treatment Mediated by Nanoparticles</p>

Diffusion Reflection Measurements of Antibodies Conjugated to Gold Nanoparticles as a Method to Identify Cutaneous Squamous Cell Carcinoma Borders

A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approach

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