The content of total polyphenolics, antioxidative capacity and antiproliferative activity were tested in wild and cultivated blackberry pomace. Wild blackberry pomace extract Tw2 showed the highest following contents: total polyphenolics (50.16 mg GAE g À1 dw), flavonoids (7.73 mg Qc g À1 dw), flavonols (6.63 mg Qc g À1 dw) and total monomeric anthocyanins (13.40 mg Cy g À1 ). Tw2 extract significantly inhibited free radicals: IC 50 DPPH = 127.76 lg mL À1 , IC 50 ABTS = 26.53 lg mL À1 and IC 50˙O H = 168.62 lg mL À1 , and the growth of breast adenocarcinoma IC 50 MCF7 = 306.68 lg mL À1 and cervix epitheloid carcinoma cell lines IC 50 HeLa = 315.49 lg mL À1 . Wild blackberry varieties had higher extraction yields, higher total polyphenolic contents and possessed stronger biological effects compared to cultivated blackberries (P < 0.05). All blackberry extracts showed high biological potential that could be attributed to high total polyphenols and flavonoids content and could be utilised as value-added functional food.
Identification and quantification of phenolic acids and flavonoids by HPLC methodChromatograms were recorded using different wavelength for individual compounds: 280 nm for hydroxybenzoic acids, ellagic acid, catechin and epicatechin, 320 nm for hydroxycinnamic acids and 360 nm for flavonoids. Separation was performed on a Luna C-18 RP column, 5 mm, 250 9 4.6 mm with a C18 guard Blackberries pomacephenolics and bioactivity M. Jazi c et al. † (mg Q equivalent g À1 fresh pomace dw). § (mg CyG equivalent g À1 fresh pomace dw).Blackberries pomacephenolics and bioactivity M. Jazi c et al.