Identification of the biosynthesis gene cluster for the novel lantibiotic paenilan fromPaenibacillus polymyxaE681 and characterization of its product

Abstract

Paenibacillus species are a good source of new lantibiotics, and the conservation of the paenilan gene among Paenibacillus sp. implies paenilan has an important function(s) for their survival.

Identification of Putative Novel Class-I Lanthipeptides in Firmicutes: A Combinatorial In Silico Analysis Approach Performed on Genome Sequenced Bacteria and a Close Inspection of Z-Geobacillin Lanthipeptide Biosynthesis Gene Cluster of the Thermophilic Geobacillus sp. Strain ZGt-1

“…Analysis of the genome sequence of strain E681 showed that it harbors a class-I lanthipeptide cluster. The antiSMASH results are in agreement with the RefSeq annotation and the analysis of Park et al [ 36 ] (details are given in S.2.3.4.1).…”

“…Analysis of the genome sequence of strain CR1 showed that it harbors a class-I lanthipeptide cluster that codes for a lanthipeptide highly similar to paenilan, which was characterized in [ 36 ]. The nt sequence of the gene coding for the predicted lanthipeptide ( Table S1 ) is 100% identical to ‘X809_RS07820’, which has been annotated on the RefSeq genome as coding for a hypothetical protein (WP_023987834) ( Table S2 ).…”

The Discovery of New Antilisterial Proteins From Paenibacillus polymyxa Kp10 via Genome Mining and Mass Spectrometry

“…The functions of the putative biosynthetic proteins encoded at the upstream and downstream of the precursor gene were further verified through conserved domain analysis using CD-Search ( Table S4 ). In comparison to the extensively studied paenibacillin, paenilan, and paeninodin produced by Paenibacillus polymyxa OSY-DF (He et al, 2008 ), Paenibacillus polymyxa E681 (Park et al, 2017 ), and Paenibacillus dendritiformis C454 (Zhu et al, 2016 ), respectively, characterization and determination of biosynthesis mechanisms of TOMM and sactipeptide in other Paenibacillus polymyxa received less attention by the researchers. TOMM is featured by the presence of thiazole and oxazole heterocycles derived from modification of cysteine and serine residues (Metelev and Ghilarov, 2014 ).…”

“… Theoretical isoelectric point and molecular weight of mature peptides (1–5; bold) were computed using Compute pI/MW tool of ExPAsy (Bioinformatics Resource Portal). Cleavage site of paenibacillin, paenilan, and lasso peptide were determined based on previous research (He et al, 2008 ; Zhu et al, 2016 ; Park et al, 2017 ). Cleavage site of sactipeptides and thiazole/oxazole modified microcins were predicted using SignalP 5.0.…”

“…A dendrogram based on ANI matrix has disclosed that Kp10 belongs to the same cluster with P. polymyxa ATCC 842, which was classified as an original P. polymyxa species (Jeong et al, 2019). In this work, the (He et al, 2008;Zhu et al, 2016;Park et al, 2017). Cleavage site of sactipeptides and thiazole/oxazole modified microcins were predicted using SignalP 5.0.…”