Hydroxylation and sulfation of sex steroid hormones in
inflammatory liver

Lee, Sang Ryong; Lee, Seungyeon; Kim, Sang-yun; Ryu, Si-Yun; Park, Bae-kuen; Hong, Eui‐Ju

doi:10.7555/jbr.31.20170031

Cited by 10 publications

(2 citation statements)

References 28 publications

(25 reference statements)

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“…CYP17A1 converts progesterone to androstenedione, which is a direct precursor of testosterone biosynthesis 40 . CYP19A1 converts testosterone to oestradiol and hydroxylation of oestradiol by CYP1B1 to produce 4-OH-oestradiol and 2-OH-oestradiol 41 . CYP1B1 can further convert 4-OH-oestradiol and 2-OH-oestradiol into quinone and semiquinone, which can produce ROS through redox cycle, resulting in oxidative damage 42 .…”

Section: Discussionmentioning

confidence: 99%

Genome-wide Transcriptional Analysis of Oxidative Stress-related Genes and Pathways Induced by CdTe aqQDs in Mice

Kong

Wang³

et al. 2018

Nanotheranostics

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Objective: Quantum dots (QDs) has widely applied in the field of science, whose potential toxic effect has increasingly become a focus concern we need pay attention to in public health. The purpose of this article was to explore the toxicity mechanism with oxidative damage from treatment with QDs at the molecular level through a gene microarray.Methods: Mice were administered aqueously synthesized cadmium telluride QDs (CdTe aqQDs) via intravenous tail injection of a 2 µmol/kg solution (based on the molar mass of Cd), and their kidneys were collected at 1 day in strict accordance with the programs used for treated mice. We determined the hierarchical clustering of expression ratios, enriched gene ontology (GO) terms and signaling pathways through gene microarray analysis and bioinformatics analysis in kidney tissue and screened the key enzyme genes, which were verified by real-time quantitative polymerase chain reaction (real-time qPCR).Results: Compared to control group, 459 lncRNAs (197 down-regulated and 262 up-regulated) and 256 mRNAs (103 down-regulated and 153 up-regulated) were differentially expressed. According to biological processes in enriched GO terms, the response to a redox state played a significant role in the biological processes involved altered genes. Pathway analysis showed that the signaling pathways that involved cytochrome P450 (CYP450) enzymes had a close relationship with QDs. Among these signaling pathways, gene expression profiling revealed that selected differentially expressed mRNAs (CYP19A1, CYP1B1, CYP11A1, CYP11B2, and CYP17A1 in the kidney and CYP19A1 and CYP1B1 in the liver) were validated by real-time qPCR, resulting in expression levels of CYP11A1, CYP11B2 and CYP17A1 in the kidney and CYP19A1 and CYP1B1 in the liver that were significantly increased, however in expression levels of CYP19A1 and CYP1B1 compared with control group in the kidney, there was no significant difference.Conclusions: Our results provide a foundation for and potential insight into the role of CYP450-related genes in QD-induced oxidative stress. QDs may produce a great deal of reactive oxygen species (ROS) by promoting high expression of CYP450 enzymes and accumulating steroid hormones, which may be an important toxicity mechanism for mediating oxidative stress and tissue damage.

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Section: Discussionmentioning

confidence: 99%

Genome-wide Transcriptional Analysis of Oxidative Stress-related Genes and Pathways Induced by CdTe aqQDs in Mice

Kong

Wang³

et al. 2018

Nanotheranostics

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“…The liver is involved in metabolism of a number of hormones, therefore its disease may be accompanied by further hormonal disorders (Tomych, 2015). In particular, inflammatory processes in the liver affect metabolism of sex steroids, and may also suppress their binding with receptors throughout the whole body (Lee et al, 2017). Research has shown the complex relation of sex hormones with the liver, because it plays a role of mediator in a number of systemic effects on human and animal organisms (Klimyuk et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Bile acids from bile of rats of different sexes under testosterone

Chernuha

Reshetnik

Liashevych

et al. 2018

Regul. Mech. Biosyst.

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Among the various functions of the liver, the formation of bile plays an important role. The optimal physiological ratio of bile components and the content of testosterone in the blood depend on various factors that can cause biliary system dysfunction and secretion. In experiments on different-sex rats, changes in bile acid contents of bile under the influence of testosterone propionate, which was injected intramuscularly 0.7 mg/kg, for 5 days were investigated. With the method of thin-layer chromatography, the basic fractions of bile acids conjugated in the bile were defined – taurocholic, taurochenodeoxycholic and taurodeoxycholic, glycocholic, glycochenodeoxycholic and glycodeoxycholic and free – cholic, chenodeoxycholic and deoxycholic acids. Conjugation rates were calculated (the ratio of the sum of conjugated cholates to the amount of free ones) and hydroxylation (ratio of the sum of trihydroxycholate bile acids to the sum of dihydroxycholanic) bile acids. In the bile of female rats almost all concentrations of cholates increased, except glycochenodeoxycholic and glycodeoxycholic acids. The calculated conjugation index on the whole did not undergo significant changes, but the hydroxylation factor increased, which may indicate an intensification of bile acid biosynthesis by neutral means, which is realized by 7α-hydroxylation of cholesterol. Under the influence of the hormone in male rats, the content of conjugated bile acids increased, and as for the free ones – a multidirectional effect of testosterone is observed, in particular, the concentration of cholic acid significantly decreased, indicating the activation of the poly-enzyme systems providing its conjugation with glycine and taurine. In connection with the wide use of the drug testosterone propionate and in view of its identified effects on the bile acid contents of the course of intramuscular administration, it is advisable to investigate the effect of this drug on the productive capacity of the liver.

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