Improvement of fermentation performance of Gluconobacter oxydans by combination of enhanced oxygen mass transfer in compressed-oxygen-supplied sealed system and cell-recycle technique

Zhou, Xin; Zhou, Xuelian; Xu, Yong

doi:10.1016/j.biortech.2017.08.107

Cited by 58 publications

(33 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since oxygen availability may become a bottleneck for the xylose-to-xylonate conversion, we next examined the effect of improved aeration through increased agitation of P. putida resting cells. In Zhou et al (2017), the increase in agitation speed from 300 to 500 rpm enhanced the accumulation of xylonate by 25%. To check whether we could observe the same trend, we incubated resting cells in minimal medium with 5 g L −1 xylose at agitation of 170 or 300 rpm and the level of xylose conversion to xylonate was determined after 48 h (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…It is worth to note that a number of studies on microbial xylonate production have reported the association of xylose oxidation to a host’s growth (Toivari et al , 2012; Köhler et al , 2014; Wang et al , 2016) but some have not. One example of the latter is recent work by Zhou and co-workers (2017) on G. oxydans , which could be used repeatedly for xylonate production in a bioreactor with an improved oxygen delivery system. Such cell recycling can be a promising strategy offering high xylonate yield and reduced process costs.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly to other naturally occurring or recombinant xylonate producers (Nygård et al , 2011; M. Toivari et al , 2012; Wang et al , 2016; Zhou et al , 2017; Gao et al ., 2019) P. putida was grown in a medium rich in amino acids and vitamins, namely in LB (La Rosa et al ., 2016). However, such complex media are expensive and thus unsuitable for large-scale bioprocesses.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Biotransformation of D-xylose to D-xylonic acid coupled to medium chain length polyhydroxyalkanoate production in cellobiose-grownPseudomonas putidaEM42

Dvořák

Kováč

Lorenzo

2019

Preprint

View full text Add to dashboard Cite

1Co-production of two or more desirable compounds from low-cost substrates by a single 2 microbial catalyst could greatly improve the economic competitiveness of many 3 biotechnological processes. However, reports demonstrating the adoption of such co-4 production strategy are still scarce. In this study, the ability of genome-edited strain 5 Psudomonas putida EM42 to simultaneously valorise D-xylose and D-cellobiose -two 6 important lignocellulosic carbohydrates -by converting them into the platform chemical D-7 xylonic acid and medium chain length polyhydroxyalkanoates, respectively, was investigated. 8 Biotransformation experiments performed with P. putida resting cells showed that 9 promiscuous periplasmic glucose oxidation route can efficiently generate extracellular 10 xylonate with high yield reaching 0.97 g per g of supplied xylose. Xylose oxidation was 11 subsequently coupled to the growth of P. putida with cytoplasmic -glucosidase BglC from 12 Thermobifida fusca on D-cellobiose. This disaccharide turned out to be a better co-substrate 13 for xylose-to-xylonate biotransformation than monomeric glucose. This was because unlike 14 glucose, cellobiose did not block oxidation of the pentose by periplasmic glucose 15 dehydrogenase Gcd, but, similarly to glucose, it was a suitable substrate for 16 polyhydroxyalkanoate formation in P. putida. Co-production of extracellular xylose-born 17 xylonate and intracellular cellobiose-born medium chain length polyhydroxyalkanoates was 18 established in proof-of-concept experiments with P. putida grown on the disaccharide. This 19 study highlights the potential of P. putida EM42 as a microbial platform for the production of 20 xylonic acid, identifies cellobiose as a new substrate for mcl-PHA production, and proposes a 21 fresh strategy for the simultaneous valorisation of xylose and cellobiose.22 23 24 3

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Biotransformation of D-xylose to D-xylonic acid coupled to medium chain length polyhydroxyalkanoate production in cellobiose-grownPseudomonas putidaEM42

Dvořák

Kováč

Lorenzo

2019

Preprint

View full text Add to dashboard Cite

show abstract

“…It is noteworthy that a number of studies on microbial xylonate production have reported the association of xylose oxidation to a host’s growth (Toivari et al , 2012a,b; Köhler et al , 2015; Wang et al , 2016) but some have not. One example of the latter is recent work by Zhou et al (2017) on G. oxydans , which could be used repeatedly for xylonate production in a bioreactor with an improved oxygen delivery system. Such cell recycling can be a promising strategy offering high xylonate yield and reduced process costs.…”

Section: Resultsmentioning

confidence: 99%

“…Instead, the transformation experiments were preceded by the production of whole‐cell catalyst biomass. Similarly to other naturally occurring or recombinant xylonate producers (Nygård et al , 2011; Toivari et al , 2012a,b; Wang et al , 2016; Zhou et al , 2017; Gao et al , 2019) , P. putida was grown in a medium rich in amino acids and vitamins, namely in LB (La Rosa et al , 2016). However, such complex media are expensive and thus unsuitable for large‐scale bioprocesses.…”

Section: Resultsmentioning

confidence: 99%

Biotransformation of d‐xylose to d‐xylonate coupled to medium‐chain‐length polyhydroxyalkanoate production in cellobiose‐grown Pseudomonas putida EM42

Dvořák

Kováč

Lorenzo

2020

Microbial Biotechnology

View full text Add to dashboard Cite

Co-production of two or more desirable compounds from low-cost substrates by a single microbial catalyst could greatly improve the economic competitiveness of many biotechnological processes. However, reports demonstrating the adoption of such co-production strategy are still scarce. In this study, the ability of genome-edited strain Pseudomonas putida EM42 to simultaneously valorize D-xylose and D-cellobiosetwo important lignocellulosic carbohydratesby converting them into the platform chemical D-xylonate and medium-chain-length polyhydroxyalkanoates, respectively, was investigated. Biotransformation experiments performed with P. putida resting cells showed that promiscuous periplasmic glucose oxidation route can efficiently generate extracellular xylonate with a high yield.Xylose oxidation was subsequently coupled to the growth of P. putida with cytoplasmic b-glucosidase BglC from Thermobifida fusca on D-cellobiose. This disaccharide turned out to be a better co-substrate for xylose-to-xylonate biotransformation than monomeric glucose. This was because unlike glucose, cellobiose did not block oxidation of the pentose by periplasmic glucose dehydrogenase Gcd, but, similarly to glucose, it was a suitable substrate for polyhydroxyalkanoate formation in P. putida. Coproduction of extracellular xylose-born xylonate and intracellular cellobiose-born medium-chain-length polyhydroxyalkanoates was established in proof-ofconcept experiments with P. putida grown on the disaccharide. This study highlights the potential of P. putida EM42 as a microbial platform for the production of xylonate, identifies cellobiose as a new substrate for mcl-PHA production, and proposes a fresh strategy for the simultaneous valorization of xylose and cellobiose.

show abstract

Gas pressure intensifying oxygen transfer to significantly improving the bio‐oxidation productivity of whole‐cell catalysis

et al. 2022

Self Cite

View full text Add to dashboard Cite

Oxygen, as a terminal electron acceptor, is an essential substrate in the aerobic biooxidation process, affecting bacterial vitality and bio-oxidation performance. In this study, a newfangled platform biotechnology of sealed-oxygen supply bioreactor (SOS-BR) was developed by improving gas pressure to significantly intensify oxygen transfer rate to resolve the formidable barriers of aerobic catalysis. In virtue of SOS-BR, the bio-productivity was greatly improved for three representative substrates (xylose, furfural, and glycerol) bio-oxidation with the whole-cell catalysis of Gluconobacter oxydans. The determination of oxygen transfer coefficient (K L α) established an upgraded theoretical dynamic model for gas pressure intensification biosystem. Additionally, viscosity measurement and combined pressure control strategy explained the inflection point phenomenon of productivity and confirmed the intensify mechanism. The smart strategy of significantly intensifying oxygen transfer provided insightful ideas for overcoming the stubborn obstacle of obligate aerobic catalysis, and further promoted the development and industrial practicability of bio-oxidation.

show abstract

Improvement of fermentation performance of Gluconobacter oxydans by combination of enhanced oxygen mass transfer in compressed-oxygen-supplied sealed system and cell-recycle technique

Cited by 58 publications

References 13 publications

Biotransformation of D-xylose to D-xylonic acid coupled to medium chain length polyhydroxyalkanoate production in cellobiose-grownPseudomonas putidaEM42

Biotransformation of D-xylose to D-xylonic acid coupled to medium chain length polyhydroxyalkanoate production in cellobiose-grownPseudomonas putidaEM42

Biotransformation of d‐xylose to d‐xylonate coupled to medium‐chain‐length polyhydroxyalkanoate production in cellobiose‐grown Pseudomonas putida EM42

Gas pressure intensifying oxygen transfer to significantly improving the bio‐oxidation productivity of whole‐cell catalysis

Contact Info

Product

Resources

About