Nanocomposite particles with improved microstructure for 3D culture systems and bone regeneration

Cecoltan, Sergiu; Stancu, Izabela‐Cristina; Dragusin, Diana Maria; Serafim, Andrada; Lungu, Adriana; Țucureanu, Cătălin; Caraş, Iuliana; Tofan, Vlad; Sălăgeanu, Aurora; Vasile, Eugeniu; Mallet, Romain; Chappard, Daniel; Coman, Cristin; Istodorescu, Mircea; Iovu, Horia

doi:10.1007/s10856-017-5966-8

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…The micrograph in Figure 7o presents the morphological details specific to nanoapatite crystals intimately combined with the organic matrix. We previously described similar morphology consisting in bundles of rod-like nanocrystallites generating a porous nanocomposite structure [27]. Such morphology suggested that the mineral was formed following intense and homogeneous Ca/P capturing by the hydrogel, followed by precipitation.…”

Section: Casein Effect On the Mineralisation Of The Hybridsmentioning

confidence: 76%

Development of New Hybrid Casein-Loaded PHEMA-PEGDA Hydrogels with Enhanced Mineralisation Potential

Dumitrescu

Serafim

Ginghină³

et al. 2022

Materials

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Casein is a micellar protein rich in glutamic and aspartic acids as well as in phosphoserine. Considering its native affinity for calcium and the connection of sub-micelles through calcium phosphate nanoclusters, this protein holds promise for stimulating biomimetic mineralisation phenomena and direct binding with the mineral phase of hard tissues. In this work we prepared new hybrids based on casein embedded in a poly(2-hydroxyethyl methacrylate)-polyethyleneglycol diacrylate (PHEMA-PEGDA) hydrogel. The resulting materials were investigated structurally by Fourier transform infrared (FT-IR). Casein modified the water affinity and the rheological properties of the hybrids. The microstructure was explored by scanning electron microscopy (SEM) and the distribution of the protein was established by combined SEM micrographs and elemental mapping considering the casein-specific elements (P, N and S) not contained by the synthetic hydrogel matrix. The effect of casein on the mineralisation potential and stability of the mineral phase was investigated by FT-IR and SEM when alternating incubation in Ca/P solutions is performed. Increasing casein content in the hybrids leads to improved mineralisation, with localised formation of nanoapatite phase on the protein areas in the richest sample in protein. This behaviour was proved microstructurally by SEM and through overlapping elemental distribution of Ca and P from the newly formed mineral and P, S and N from the protein. This study indicates that nanoapatite-casein-PHEMA-PEGDA nanocomposites may be developed for potential use in bone repair and regeneration.

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Section: Casein Effect On the Mineralisation Of The Hybridsmentioning

confidence: 76%

Development of New Hybrid Casein-Loaded PHEMA-PEGDA Hydrogels with Enhanced Mineralisation Potential

Dumitrescu

Serafim

Ginghină³

et al. 2022

Materials

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“…With tight control over the instantaneous magnitude release rate, pulsatile release patterns may be created. [ 45 ]…”

Section: Physicochemical Properties Of Hydrogelsmentioning

confidence: 99%

Cancer Immunotherapy Using Bioengineered Micro/Nano Structured Hydrogels

Askari,

Shokrollahi Barough,

Rahmanian

et al. 2023

Adv Healthcare Materials

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Hydrogels, a class of materials with a three‐dimensional network structure, are widely used in various applications of therapeutic delivery, particularly cancer therapy. Micro and nanogels as a miniaturized structure of the bioengineered hydrogels could provide extensive benefits over the common hydrogels in encapsulation and controlled release of small molecular drugs, macromolecular therapeutics, and even cells. Cancer immunotherapy is rapidly developing, and micro/nanostructured hydrogels have gained wide attention regarding their engineered payload release properties that enhance systemic anticancer immunity. Additionally, they are a great candidate due to their local administration properties with the focus on local immune cells manipulation in favor of active and passive immunotherapies. Although applied locally, such micro/nanostructured can also activate systemic antitumor immune responses by releasing nanovaccines safely and effectively inhibiting tumor metastasis and recurrence. However, such hydrogels are mostly used as local administered carriers to stimulate the immune cells by releasing tumor lysate, drugs, or nanovaccines. In this review, we summarize the latest developments in cancer immunotherapy using micro/nanostructured hydrogels with a particular emphasis on their function depending on the administration route. Moreover, the potential for clinical translation of these hydrogel‐based cancer immunotherapies is also discussed.This article is protected by copyright. All rights reserved

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“…As opposed to other techniques, CT provides a general overview of either dried or hydrated samples, thus increasing its importance in scaffold characterization for biomedical applications. Despite this significant advantage, only a relatively small number of papers report the evaluation in hydrated state [69][70][71][72] due to the difficulty of obtaining a good X-ray contrast between the hydrated matrix and immersion media.…”

Section: Visualization Of Architectural Features Through Ct Imagingmentioning

confidence: 99%

Computed Tomography as a Characterization Tool for Engineered Scaffolds with Biomedical Applications

et al. 2021

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The ever-growing field of materials with applications in the biomedical field holds great promise regarding the design and fabrication of devices with specific characteristics, especially scaffolds with personalized geometry and architecture. The continuous technological development pushes the limits of innovation in obtaining adequate scaffolds and establishing their characteristics and performance. To this end, computed tomography (CT) proved to be a reliable, nondestructive, high-performance machine, enabling visualization and structure analysis at submicronic resolutions. CT allows both qualitative and quantitative data of the 3D model, offering an overall image of its specific architectural features and reliable numerical data for rigorous analyses. The precise engineering of scaffolds consists in the fabrication of objects with well-defined morphometric parameters (e.g., shape, porosity, wall thickness) and in their performance validation through thorough control over their behavior (in situ visualization, degradation, new tissue formation, wear, etc.). This review is focused on the use of CT in biomaterial science with the aim of qualitatively and quantitatively assessing the scaffolds’ features and monitoring their behavior following in vivo or in vitro experiments. Furthermore, the paper presents the benefits and limitations regarding the employment of this technique when engineering materials with applications in the biomedical field.

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Nanocomposite particles with improved microstructure for 3D culture systems and bone regeneration

Cited by 7 publications

References 18 publications

Development of New Hybrid Casein-Loaded PHEMA-PEGDA Hydrogels with Enhanced Mineralisation Potential

Development of New Hybrid Casein-Loaded PHEMA-PEGDA Hydrogels with Enhanced Mineralisation Potential

Cancer Immunotherapy Using Bioengineered Micro/Nano Structured Hydrogels

Computed Tomography as a Characterization Tool for Engineered Scaffolds with Biomedical Applications

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