Do not forget about pre-analytics in faecal calprotectin measurement!

Oyaert, Matthijs; Bremt, Stefanie Van den; Boel, An; Bossuyt, Xavier; Hoovels, Lieve Van

doi:10.1016/j.cca.2017.08.025

Cited by 18 publications

(7 citation statements)

References 8 publications

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“…FC extracts stored in the fridge (2–8 °C) were only stable for up to 2 days, while they could be stored at −20 °C for up to 4 weeks without significant variation. These results are indeed in accordance with other studies regarding the good conservation at −20 °C [ 13 , 29 ] as well as the lack of stability in the fridge [ 30 , 31 ].…”

Section: Discussionsupporting

confidence: 93%

New Faecal Calprotectin Assay by IDS: Validation and Comparison to DiaSorin Method

et al. 2022

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Background: The faecal calprotectin (FC) measurement is used for inflammatory bowel disease (IBD) diagnosis and follow-up. The aim of this study was to validate for the first time the new IDS FC extraction device and immunoassay kit, and to compare it with the DiaSorin test in patients with and without IBD. Methods: First, the precision of the IDS assay and its stability were assessed. Then, 379 stool extracts were analysed with the IDS kit on iSYS and compared with a DiaSorin Liaison XL assay. Results: The intra- and inter-assay CVs did not exceed 5%. The extracts were stable up to 4 weeks at −20 °C. Lot-to-lot comparison showed a good correlation (Lot1 = 1.06 × Lot2 + 0.60; p > 0.05). The Passing and Bablok regression showed no significant deviation from linearity between the two methods (IDS = 1.06 × DiaSorin − 0.6; p > 0.05; concordance correlation coefficient = 0.93). According to the recommended cut-offs, the IDS assay identified more IBD and irritable bowel syndrome patients than DiaSorin, which had more borderline results (16 vs. 20%, respectively). Conclusions: The IDS faecal calprotectin had good analytical validation parameters. Compared to the DiaSorin method, it showed comparable results, but slightly outperformed it in the identification of more IBD patients and active disease.

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Section: Discussionsupporting

confidence: 93%

New Faecal Calprotectin Assay by IDS: Validation and Comparison to DiaSorin Method

et al. 2022

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“…On the other hand, it is not also recommended to keep samples in the refrigerator for more than 7 days [28] ,. It seems that fecal calprotectin remains stable up to one year at −20 °C [29] ,. Measurement of fecal calprotectin can be done both qualitatively and quantitatively.…”

Section: The Role Of Fecal Calprotectin In Diagnosis and Management Omentioning

confidence: 95%

Calprotectin in inflammatory bowel disease

Khaki‐Khatibi

Qujeq

Kashifard

et al. 2020

Clinica Chimica Acta

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Highlights The prevalence of IBD is increasing in many societies. The need for an appropriate biomarker for the diagnosis and management of IBD is felt. Fecal Calprotectin is helpful biomarker in discrimination between IBD and IBS. Fecal calprotectin showed significant correlation with some endoscopic activity indices. In COVID-19 pandemic, fecal calprotectin can be helpful for IBD non-invasive management. In interpreting the results of fecal calprotectin test, test limitations should be considered.

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“…Fecal calprotectin (FC) accounts for 60% of the cytoplasmic protein concentration of neutrophils and macrophages (32) and is considered the most reliable predictor of short-term recurrence and inflammatory activity in IBD (33)(34)(35). However, the cut-off value for fecal calprotectin has always been controversial and is affected by numerous factors including time of defecation (36), patient age (37), diet and lifestyle (38,39), disease, medication usage (40), and storage conditions (36,41). In addition, many studies have reported different cut-off values for fecal calprotectin, ranging from 100 to 250 µg/g (42,43), such that only a moderate prediction of disease can be made for individual patients.…”

Section: Traditional Plasma and Stool Biomarkers Used To Assess Ibd Activitymentioning

confidence: 99%

Gut Microbiota Is a Potential Biomarker in Inflammatory Bowel Disease

Guo

Huang

et al. 2022

Front. Nutr.

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Inflammatory bowel disease (IBD), which includes ulcerative colitis (UC) and Crohn's disease (CD), is characterized by relapse and remission alternately. It remains a great challenge to diagnose and assess disease activity during IBD due to the lack of specific markers. While traditional biomarkers from plasma and stool, such as C-reactive protein (CRP), fecal calprotectin (FC), and S100A12, can be used to measure inflammation, they are not specific to IBD and difficult to determine an effective cut-off value. There is consensus that gut microbiota is crucial for intestinal dysbiosis is closely associated with IBD etiopathology and pathogenesis. Multiple studies have documented differences in the composition of gut microbiota between patients with IBD and healthy individuals, particularly regarding microbial diversity and relative abundance of specific bacteria. Patients with IBD have higher levels of Proteobacteria and lower amounts of Bacteroides, Eubacterium, and Faecalibacterium than healthy individuals. This review summarizes the pros and cons of using traditional and microbiota biomarkers to assess disease severity and treatment outcomes and addresses the possibility of using microbiota-focused interventions during IBD treatment. Understanding the role of microbial biomarkers in the assessment of disease activity and treatment outcomes has the potential to change clinical practice and lead to the development of more personalized therapies.

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Do not forget about pre-analytics in faecal calprotectin measurement!

Cited by 18 publications

References 8 publications

New Faecal Calprotectin Assay by IDS: Validation and Comparison to DiaSorin Method

New Faecal Calprotectin Assay by IDS: Validation and Comparison to DiaSorin Method

Calprotectin in inflammatory bowel disease

Gut Microbiota Is a Potential Biomarker in Inflammatory Bowel Disease

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