Polydeoxyribonucleotide Ameliorates Lipopolysaccharide-Induced Lung Injury by Inhibiting Apoptotic Cell Death in Rats

An, Jin; Park, So Hee; Ko, Il‐Gyu; Jin, Jiaqi; Hwang, Lakkyong; Ji, Eun Sun; Kim, Sang Hoon; Park, So Young; Hwang, Jae Joon; Choi, Cheon Woong

doi:10.3390/ijms18091847

Cited by 29 publications

(25 citation statements)

References 38 publications

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“…In general, ROS generation and complement activation occur early during reperfusion 24 . It has been stated that lung injury was characterized in histological sections with diffuse lung inflammation, alveolar-capillary destruction, and alveolar flooding, resulting in respiratory failure 25,26 . In our histological study, we found lung injury such as intra-alveolar hemorrhage, inflammatory cell infiltration in the contol-saline+IR group.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the protective effect of enoxaparin and ticagrelor pretreatment against ischemia-reperfusion injury in rat lung tissue

Findik¹,

Yilmaz

Yazır

et al. 2019

Rev. Assoc. Med. Bras.

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SUMMARY OBJECTIVES This study was conducted to reveal the possible protective effects of ticagrelor and enoxaparin pretreatment against ischemia-reperfusion (IR)-induced injury on the lung tissue of a rat model. METHODS Wistar albino rats were randomly divided into 4 groups as follows: group-1 (control-sham), group-2 (control-saline+IR), group-3 (ticagrelor+IR), group-4 (enoxaparin+IR). Before the ischemic period, saline, ticagrelor, and enoxaparin were administered to the 2nd-4th groups, respectively. In these groups, IR injury was induced by clamping the aorta infrarenally for 2 h, followed by 4 h of reperfusion except group-1. After the rats were euthanized, the lungs were processed for histological examinations. Paraffin sections were stained with Haematoxylin&Eosin (H&E) for light microscopic observation. Apoptosis was evaluated by caspase-3 immunoreactivity. Data were statistically analyzed using the SPSS software. RESULTS In the lung sections stained with H&E, a normal histological structure was observed in group-1, whereas disorganized epithelial cells, hemorrhage, and inflammatory cell infiltration were seen in the alveolar wall in group-2. The histologic structure of the treatment groups was better than that of group-2. Caspase-3(+) apoptotic cells were noticeable in sections of group-2 and were lower in the treatment groups. In group-4, caspase-3 immunostaining was lower than in group-3. In group-2, apoptotic cells were significantly higher than in the other groups (p<0.001). CONCLUSION Based on the histological results, we suggested that both therapies ameliorated the detrimental effects of IR. Caspase-3 immunohistochemistry results also revealed that pre-treatment with enoxaparin gave better results in an IR-induced rat injury model. In further studies, other parameters such as ROS and inflammatory gene expressions should be evaluated for accurate results.

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Section: Discussionmentioning

confidence: 99%

Investigation of the protective effect of enoxaparin and ticagrelor pretreatment against ischemia-reperfusion injury in rat lung tissue

Findik¹,

Yilmaz

Yazır

et al. 2019

Rev. Assoc. Med. Bras.

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“…Recent evidence has suggested that the main pathogenic mechanisms of ALI include oxidative stress, cytokine release, inflammation and apoptosis [36]. During ALI, large amounts of ROS are generated, which cause cell membrane lipid peroxidation and the destruction of lung parenchymal cells.…”

Section: Discussionmentioning

confidence: 99%

Pulicaria petiolaris effectively attenuates lipopolysaccharide (LPS)-induced acute lung injury in mice

Ahmed

Aljuhani

Salamah

et al. 2018

ARCH BIOL SCI BELGRA

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Members of the genus Pulicaria have been used in traditional medicine for alleviating several complaints as they have a rich pool of biometabolites. Acute lung injury (ALI) is a serious disease with an elevated mortality rate. The present investigation aimed to evaluate the total phenolic and flavonoid contents and antioxidant capacity of the methanolic extract of P. petiolaris Jaub. and Spach. (PP) (Asteraceae). Moreover, the potential protective potential of PP against lipopolysaccharide-(LPS)-induced ALI was assessed. PP is a rich source of phenolics and flavonoids. The total phenolic content (TPC) was 68.05 mg gallic acid equivalent (GAE)/g dried extract, and the total flavonoid content (TFC) was 45.86 mg quercetin equivalent (QE)/g dried extract. Additionally, PP possessed a promising DPPH-scavenging activity, with an IC 50 =27 μg/mL. Our results showed that PP lessened LPS-induced lung injury. PP effectively reduced pulmonary edema as it lowered total protein and the lung wet/dry weight (W/D) ratio in the bronchoalveolar lavage fluid (BALF). It also significantly ameliorated the level of lactate dehydrogenase (LDH) in the BALF and improved the histopathological lesions in the lung tissue. LPS-induced inflammatory cell infiltration was greatly depressed in PP-treated animals. PP showed antioxidant capacity as it reduced the LPS-induced increase in the lipid peroxidation marker, malondialdehyde (MDA). It also increased the activity of superoxide dismutase (SOD) and the content of reduced glutathione (GSH). This study indicates that PP significantly decreased LPS-induced inflammation in the LPS-mediated ALI murine model, suggesting that it may become a significant preventive strategy for treating nonspecific inflammation of the lungs.

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“…The first group of articles analyzes different possible pathways of the immune and inflammatory response, before proposing possible diagnostic and treatment interventions [2][3][4][5]. Douglas et al [2] analyzed the evidence from the literature on the enhancement of upper respiratory innate immunity due to bitter taste receptors and the possible roles of individual taste differences in the clinical management of patients with upper respiratory infections.…”

Section: Reviewmentioning

confidence: 99%

Lung Diseases: Chronic Respiratory Infections

2018

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Polydeoxyribonucleotide Ameliorates Lipopolysaccharide-Induced Lung Injury by Inhibiting Apoptotic Cell Death in Rats

Cited by 29 publications

References 38 publications

Investigation of the protective effect of enoxaparin and ticagrelor pretreatment against ischemia-reperfusion injury in rat lung tissue

Investigation of the protective effect of enoxaparin and ticagrelor pretreatment against ischemia-reperfusion injury in rat lung tissue

Pulicaria petiolaris effectively attenuates lipopolysaccharide (LPS)-induced acute lung injury in mice

Lung Diseases: Chronic Respiratory Infections

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