Pancreatic islet macroencapsulation using microwell porous membranes

Skrzypek, Katarzyna; Nibbelink, Milou Groot; Lente, Jéré van; Buitinga, Mijke; Engelse, Marten A.; Koning, Eelco J.P. de; Karperien, Marcel; Apeldoorn, Aart van; Stamatialis, Dimitrios

doi:10.1038/s41598-017-09647-7

Cited by 50 publications

(51 citation statements)

References 47 publications

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“…The samples in receptor chamber were collected and analyzed using glucose assay kit (GAGO‐20, Sigma‐Aldrich). The glucose diffusion coefficient was calculated as previously described …”

Section: Methodsmentioning

confidence: 99%

Development of a Coaxial 3D Printing Platform for Biofabrication of Implantable Islet‐Containing Constructs

Liu

Carter

Renes

et al. 2019

Adv Healthcare Materials

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Over the last two decades, pancreatic islet transplantations have become a promising treatment for Type I diabetes. However, although providing a consistent and sustained exogenous insulin supply, there are a number of limitations hindering the widespread application of this approach. These include the lack of sufficient vasculature and allogeneic immune attacks after transplantation, which both contribute to poor cell survival rates. Here, these issues are addressed using a biofabrication approach. An alginate/gelatin‐based bioink formulation is optimized for islet and islet‐related cell encapsulation and 3D printing. In addition, a custom‐designed coaxial printer is developed for 3D printing of multicellular islet‐containing constructs. In this work, the ability to fabricate 3D constructs with precise control over the distribution of multiple cell types is demonstrated. In addition, it is shown that the viability of pancreatic islets is well maintained after the 3D printing process. Taken together, these results represent the first step toward an improved vehicle for islet transplantation and a potential novel strategy to treat Type I diabetes.

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“…The samples in receptor chamber were collected and analyzed using glucose assay kit (GAGO‐20, Sigma‐Aldrich). The glucose diffusion coefficient was calculated as previously described …”

Section: Methodsmentioning

confidence: 99%

Development of a Coaxial 3D Printing Platform for Biofabrication of Implantable Islet‐Containing Constructs

Liu

Carter

Renes

et al. 2019

Adv Healthcare Materials

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“…A new approach to increase effectivity of macroencapsulation was published by Skrzypek et al Authors constructed devise based on thin microwell membranes, composed of polyvinylpyrrolidone, providing sufficient transport of nutrients. Additionally, the separate seeding of islets into the microwells prevented their aggregation [57]. The group of Carlsson et al solved the problem with insufficient oxygen supply by construction the βAir device based on incorporation of refillable oxygen tank.…”

Section: Conclusion: Challenges and Perspectives On Ipsc-derived β-Cellsmentioning

confidence: 99%

Generation of Pancreatic β-cells From iPSCs and their Potential for Type 1 Diabetes Mellitus Replacement Therapy and Modelling

Csöbönyeiová

Polák

Danišovič

2018

Exp Clin Endocrinol Diabetes

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Diabetes type 1 (T1D) is a common autoimmune disease characterized by permanent destruction of the insulin-secreting β-cells in pancreatic islets, resulting in a deficiency of the glucose-lowering hormone insulin and persisting high blood glucose levels. Insulin has to be replaced by regular subcutaneous injections, and blood glucose level must be monitored due to the risk of hyperglycemia. Recently, transplantation of new pancreatic β-cells into T1D patients has come to be considered one of the most potentially effective treatments for this disease. Therefore, much effort has focused on understanding the regulation of β-cells. Induced pluripotent stem cells (iPSCs) represent a valuable source for T1D modelling and cell replacement therapy because of their ability to differentiate into all cell types . Recent advances in stem cell-based therapy and gene-editing tools have enabled the generation of functionally adult pancreatic β-cells derived from iPSCs. Although animal and human pancreatic development and β-cell physiology have significant differences, animal models represent an important tool in evaluating the therapeutic potential of iPSC-derived β-cells on type 1 diabetes treatment. This review outlines the recent progress in iPSC-derived β-cell differentiation methods, disease modelling, and future perspectives.

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“…The results reveal that the device was effective in maintaining islet responsiveness and function in cell culture. However, future studies need to be conducted to test the in-vivo success of such a device [39].…”

Section: Biomaterials In Transplantationmentioning

confidence: 99%

Physical Protection of Pancreatic Islets for Transplantation

Lee¹,

Sathialingam²,

Alexander³

et al. 2018

Biomaterials - Physics and Chemistry - New Edition

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Type 1 diabetes is an autoimmune disorder that destroys the insulin producing cells of the pancreas. The mainstay of treatment is replacement of insulin through injectable exogenous insulin. Improvements in islet isolation techniques and immunosuppression regimens have made islet transplants a treatment options for select patients. Islet transplants have improved graft function over the years, however, graft function beyond year two is rare and notably these patients require immunosuppression to prevent rejection. Cell encapsulation has been proposed for numerous cell types but it has found increasing enthusiasm for islets. Since islet transplants have experienced a myriad of success the next step is to improve graft function and avoid systemically toxic immunosuppressive regimens. Cell encapsulation hopes to accomplish this goal. Encapsulation involves encasing cells in a semipermeable biocompatible hydrogel that allows the passage of nutrients and oxygen however blocks immune regulators from destroying the cell thus avoiding systemic drugs. Several advances in encapsulation engineering and cell viability promises to make this a revolutionary discovery. In this chapter, we will provide a review of islet encapsulation as used for the treatment of type 1 diabetes.

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Pancreatic islet macroencapsulation using microwell porous membranes

Cited by 50 publications

References 47 publications

Development of a Coaxial 3D Printing Platform for Biofabrication of Implantable Islet‐Containing Constructs

Development of a Coaxial 3D Printing Platform for Biofabrication of Implantable Islet‐Containing Constructs

Generation of Pancreatic β-cells From iPSCs and their Potential for Type 1 Diabetes Mellitus Replacement Therapy and Modelling

Physical Protection of Pancreatic Islets for Transplantation

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