Single Versus Dual Platform Analysis for Hematopoietic Stem Cell Enumeration Using ISHAGE Protocol

Abstract

Hematopoietic stem cell transplantation is curative therapy in benign and malignant diseases. Adequate stem cell dose is one of the most important marker of engraftment. Several methods have been developed to enumerate CD34+ cells. We present our data on 147 samples analysis. There was a clear linear correlation between two methods. Both methods were effective. Both single vs dual platform analysis yield similar results. Single platform analysis is easier to perform. In terms of cost reduction dual platform analysis is better.

Hematopoietic stem cell transplantation using non-cryopreserved peripheral blood stem cells graft is effective in multiple myeloma and lymphoma

“…The harvested stem cells were stored in a conventional blood bank refrigerator at 4°C. Stem cell yield of the harvest was determined by CD 34 cell enumeration using ISHAGE protocol [9]. The goal of apheresis was to collect an average of 2 × 10 6 /kg CD 34 stem cells.…”

Highlights of Abstracts on Hematopoietic Stem Cell Transplant in Annual Conference of ISHBT 2018

“…New techniques have been studied to reduce the turnaround time and cost of investigations. Recently our center had published our results comparing single versus dual platform analysis methods for CD 34 enumeration [27]. We found that both methods were comparable.…”

Flow cytometric enumeration of CD34+ hematopoietic stem cells: A comparison between single- versus dual-platform methodology using the International Society of Hematotherapy and Graft Engineering protocol

“…used mean of TLC from two hematology analyzers. [9] However, in the present study, we used only one hematology analyzer to determine TLC, which was one of our limitations.…”