Cell metabolomics reveals the neurotoxicity mechanism of cadmium in PC12 cells

Zong, Li; Xing, Junpeng; Liu, Shu; Liu, Zhiqiang; Song, Fengrui

doi:10.1016/j.ecoenv.2017.08.028

Cited by 63 publications

(23 citation statements)

References 35 publications

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“…Accordingly, mice subjected to CUMS had elevated levels of these cytokines; however, the levels in the BA-treated group were significantly lower than those in the CUMS model group and were similar to the levels in mice given the antidepressant Flu. Similar anti-inflammatory effects of BA were observed in corticosterone-treated PC12 cells, which are neuron-like cells expressing high levels of the renal glucocorticoid receptors that play major roles in nervous system development (33). BA reduced the levels of proinflammatory cytokines and ROS and was neuroprotective against the corticosterone-induced loss of cell viability.…”

Section: Discussionmentioning

confidence: 59%

“…PC12 cells are neuron-like cells that express high levels of the renal glucocorticoid receptors that contribute to nervous system development (28). PC12 cells were cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum and 1% antibiotics (penicillin/streptomycin) in a humidified incubator containing 95% air and 5% CO 2 at 37°C.…”

Section: Methodsmentioning

confidence: 99%

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Baicalin ameliorates chronic mild stress-induced depression-like behaviors in mice and attenuates inflammatory cytokines and oxidative stress

Zhong

et al. 2019

Braz J Med Biol Res

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The natural flavonoid glycoside baicalin (BA) produces a variety of pharmaceutical effects, particularly for psychiatric/neurological disorders. This study evaluated the behavioral and neuroprotective effects of BA in mice subjected to chronic unpredictable mild stress, a model of depression. BA (25 and 50 mg/kg) significantly increased sucrose consumption and reduced immobility times in the tail suspension and forced swim tests, demonstrating that BA alleviated depression-like behaviors. Moreover, BA reduced the levels of inflammatory cytokines, such as interleukin 1β, interleukin 6, and tumor necrosis factor α, in serum and in the hippocampus. BA also abrogated increases in NMDAR/NR2B and Ca 2+ /calmodulin-dependent protein kinase II, and the decrease in phosphorylated ERK and reactive oxygen species production in mice subjected to chronic unpredictable mild stress. These findings suggested that the antidepressive effects of BA are due to the regulation of an NMDAR/NR2B-ERK1/2-related pathway and inhibition of inflammatory cytokines and oxidative stress. Thus, BA represents a potential candidate drug for patients suffering from depression.

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Section: Discussionmentioning

confidence: 59%

Section: Methodsmentioning

confidence: 99%

Baicalin ameliorates chronic mild stress-induced depression-like behaviors in mice and attenuates inflammatory cytokines and oxidative stress

Zhong

et al. 2019

Braz J Med Biol Res

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“…Glycerophospholipids are the main lipid constituents of cell membranes, which can directly affect the physiological functions of cells [44]. Changes in the glycerophospholipid metabolism pathway rst prompted the transformations of cell membrane composition and permeability.…”

Section: Discussionmentioning

confidence: 99%

Landscape of Lipidomic Metabolites in Gut-liver Axis of Sprague-Dawley Rats after Oral Exposure to Titanium Dioxide Nanoparticles

Han¹,

Zheng²,

Zhang³

et al. 2020

Preprint

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Background: The application of titanium dioxide nanoparticles (TiO2 NPs) as food additives poses a risk of oral exposure that may lead to adverse health effects. Even though the substantial evidence supported liver as the target organ of TiO2 NPs via oral exposure, the mechanism of liver toxicity remains largely unknown. Since the liver is a key organ for lipid metabolism, this study focused on the landscape of lipidomic metabolites in gut-liver axis of Sprague Dawley (SD) rats exposed to TiO2 NPs at 0, 2, 10, 50 mg/kg body weight per day for 90 days.Results: TiO2 NPs (50 mg/kg) caused slight hepatotoxicity and changed lipidomic signatures of main organs or systems in the gut-liver axis including liver, serum and gut. The cluster profile from the above biological samples all pointed to the same key metabolic pathway and metabolites, which was glycerophospholipid metabolism and Phosphatidylcholines (PCs), respectively. In addition, absolute quantitative lipidomics verified the changes of three PCs concentrations, including PC(16:0/20:1), PC(18:0/18:0) and PC(18:2/20:2) in the serum samples after treatment of TiO2 NPs (50 mg/kg). The contents of malondialdehyde (MDA) in serum and liver increased significantly, which were positively correlated with most differential lipophilic metabolites.Conclusions: The gut was presumed to be the original site of oxidative stress and disorder of lipid metabolism, which resulted in hepatotoxicity through the gut-liver axis. Lipid peroxidation may be the initial step of lipid metabolism disorder induced by TiO2 NPs. Most nanomaterials (NMs) have oxidation induction and antibacterial properties, so the toxic pathway revealed in the present study may be primary and universal.

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“…PLOS ONE [26]. Decreasing the PC content affected the integrity of liver cells and mitochondrial membrane, thus directly leading to proliferation, differentiation and apoptosis [27].…”

Section: Glycerophospholipid Metabolism and Linoleic Acid Metabolismmentioning

confidence: 99%

Hepatopancreatic metabolomics shedding light on the mechanism underlying unsynchronized growth in giant freshwater prawn, Macrobrachium rosenbergii

et al. 2020

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The giant freshwater prawn, Macrobrachium rosenbergii (M. rosenbergii) as an important freshwater aquaculture species with high commercial value, exhibited unsynchronized growth. However, the potentially metabolic mechanism remains unclear. In this study, we used liquid chromatography tandem mass spectrometry (LC-MS/MS) to investigate the hepatopancreatic metabolic profiles of twenty giant freshwater prawns between the fast-growing group and slow-growing group. In the metabolomics assay, we isolated 8,293 peaks in positive and negative iron mode. Subsequently, 44 significantly differential metabolites were identified. Functional pathway analysis revealed that these metabolites were significantly enriched in three key metabolic pathways. Further integrated analysis indicated that glycerophospholipid metabolism and aminoacyl-tRNA biosynthesis have significant impact on growth performance in M.rosenbergii. Our findings presented here demonstrated the critical metabolites and metabolic pathways involved in growth performance, moreover provided strong evidence for elucidating the potentially metabolic mechanism of the unsynchronized growth in M. rosenbergii.

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Cell metabolomics reveals the neurotoxicity mechanism of cadmium in PC12 cells

Cited by 63 publications

References 35 publications

Baicalin ameliorates chronic mild stress-induced depression-like behaviors in mice and attenuates inflammatory cytokines and oxidative stress

Baicalin ameliorates chronic mild stress-induced depression-like behaviors in mice and attenuates inflammatory cytokines and oxidative stress

Landscape of Lipidomic Metabolites in Gut-liver Axis of Sprague-Dawley Rats after Oral Exposure to Titanium Dioxide Nanoparticles

Hepatopancreatic metabolomics shedding light on the mechanism underlying unsynchronized growth in giant freshwater prawn, Macrobrachium rosenbergii

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