Determination of Fusarium toxins in functional vegetable milks applying salting-out-assisted liquid–liquid extraction combined with ultra-high-performance liquid chromatography tandem mass spectrometry

Hamed, Ahmed M.; Arroyo‐Manzanares, Natalia; Garcı́a-Campaña, Ana M.; Gámiz‐Gracia, Laura

doi:10.1080/19440049.2017.1368722

Cited by 23 publications

(11 citation statements)

References 25 publications

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“…Several works studied the occurrence of different mycotoxins in oat, soy, and rice-based milks, as they are the most consumed worldwide [32][33][34][35]. Miró-Abella et al [32] reported, for the first time, the presence of mycotoxins in plant-based milks and found DON, OTA, ZEN, and T-2 toxin together with aflatoxins B1, B2, G1, and G2 present in certain samples, with oat-based beverages being the most frequently contaminated.…”

Section: Plant-based Milksmentioning

confidence: 99%

Mycotoxins in Functional Beverages: A Review

2020

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Consumer dietary habits have drastically changed in recent decades and functional beverages now have a strong position in the market. The majority of these beverages are produced using simple processes that use raw products, such as cereals, legumes, fruits, and nuts, among others, and these are known to be frequently contaminated with mycotoxins. This review is focused on the occurrence of these toxic compounds in plant-based milks, fruit juices, and herbal teas. The fate of the toxins during processing is discussed to establish the potential risk posed by the consumption of these kind of beverages regarding mycotoxin uptake.

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Section: Plant-based Milksmentioning

confidence: 99%

Mycotoxins in Functional Beverages: A Review

2020

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“…Traditionally, mycotoxin determination has mainly been performed by chromatographic techniques, including HPLC-UV/Vis [ 15 ], HPLC-FLD [ 16 ] or liquid chromatography/mass spectrometry (LC/MS) [ 17 ], in combination with effective separation approaches to avoid matrix disturbance. Most pretreatment approaches involve liquid-liquid extraction [ 18 ], solid-phase extraction [ 19 ], liquid-phase microextration [ 20 ] or an immune-affinity column [ 21 ]. However, these methods typically suffer from some limitations, such as low sensitivity, poor repeatability, and increased cost and time.…”

Section: Introductionmentioning

confidence: 99%

Determination of Multi-Class Mycotoxins in Tartary Buckwheat by Ultra-Fast Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry

Ren

Zhang

et al. 2018

Toxins

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Considering crops are susceptible to toxicogenic fungi during plantation, pre-processing and storage, an ultra-fast liquid chromatography coupled with triple quadrupole mass spectrometry (UFLC-QTrap-MS/MS) method was developed and validated for simultaneous determination of the 12 most frequent mycotoxins, including aflatoxin B 1 , B 2 , G 1 , G 2 , HT-2, T-2 toxin, ochratoxin A, fumonisin B 1 , B 2 , zearalanone, zearalenone, and deoxynivalenol, in 14 batches of Tartary buckwheat cultivar, collected from different origins in Sichuan Province, China. Differing from those complicated approaches, a simple and cost-efficient pretreatment method based on dilute-and-shoot was employed. Based on optimized chromatographic and mass spectrometry conditions, these 12 mycotoxins could be analyzed with high correlation coefficients (all over 0.995), high precision (RSD 0.47-9.26%), stability (RSD 0.72-11.36%), and recovery (79.52% to 108.92%, RSD 4.35-14.27%). Furthermore, this analysis method exhibited good determination performance with little disturbance of the matrix effect. Finally, this proposed method was applied for 14 batches of Tartary buckwheat seeds, in which aflatoxin B 1 (AFB 1 ) was detected in one moldy cultivar, Meigu No. 2, with its concentration exceeding the maximum residue limits set by EU regulations. The method thus established, which has significant advantages, could provide a preferred determination approach candidate for measurement of multiple mycotoxins measurement in Tartary buckwheat, even other kinds of foodstuffs.

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“…On the other hand, one of the main advantages of mass spectrometry detectors is the possibility of performing multiplex analysis, not only for different mycotoxins [48,49,50,53,54,57,58,59,60,61,64,65,66,67,71,72,73,74,77,79,80,187], but also when combined with varied metabolites. Growth regulators, antibiotics, pesticides [51,70,75], and other fungal metabolites [63,68], were simultaneously identified in analysis capable of assessing up to 74 and 90 compounds [62,78]. Mass spectrometry has also been used for assessing mycotoxin transfer from feed to organs and tissue in poultry [80] The importance of novel analytical methods relies on the low sensitivities achieved within a relatively short detection time.…”

Section: Chromatographic Detection Of Fumonisin B1mentioning

confidence: 99%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida

Gong²,

Goycoolea³

2020

Preprint

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Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become a relevant compound because of its adverse effects in the food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD and ELISA, while novel approaches integrate different sensing platforms and fluorescently labelled agents in combination with antibodies. Nevertheless, such methods could be expensive, time-consuming and require experience. Aptamers (ssDNA) are promising alternatives to overcome some of the drawbacks of conventional analytical methods, their high affinity through specific aptamer-target binding has been exploited in various designs attaining favorable limits of detection (LOD). So far, two aptamers specific to FB1 have been reported, and their modified and shortened sequences have been explored for a successful target quantification. In this critical review spanning the last eight years, we have conducted a systematic comparison based on principal component analysis of the aptamer-based techniques for FB1, compared with chromatographic, immunological and other analytical methods. We have also conducted an <i>in-silico</i> prediction of the folded structure of both aptamers under their reported conditions. The potential of aptasensors for the future development of highly sensitive FB1 testing methods is emphasized.

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Determination of Fusarium toxins in functional vegetable milks applying salting-out-assisted liquid–liquid extraction combined with ultra-high-performance liquid chromatography tandem mass spectrometry

Cited by 23 publications

References 25 publications

Mycotoxins in Functional Beverages: A Review

Mycotoxins in Functional Beverages: A Review

Determination of Multi-Class Mycotoxins in Tartary Buckwheat by Ultra-Fast Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

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