Phenotypes, antioxidant responses, and gene expression changes accompanying a sugar-only diet in Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Chen, Er-Hu; Hou, Qiu‐Li; Wei, Dandan; Jiang, Hong‐Bo; Wang, Jinjun

doi:10.1186/s12862-017-1045-5

Cited by 15 publications

(10 citation statements)

References 75 publications

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“…On this high protein diet, there was one differentially upregulated KEGG pathway (p< 0.01) and seven differentially downregulated KEGG pathways (p< 0.01) in Alstonville compared to Dahomey larvae ( Fig 7A and S3A & S3B Table ). Of those upregulated in Dahomey, at least two (MXC P450 and Drug Metabolism C P450) are linked with a stress response [ 126 ], suggesting these larvae were oxidatively stressed. Gene Ontology analyses of the two mitotypes on the 1:2 P:C diet ( S4 Table ) shows the molecular function of “pyruvate dehydrogenase kinase activity” (GO:0004740) is reduced in Dahomey ( S4B Table ), suggesting reduced activity of the electron transport system in these larvae on this diet.…”

Section: Resultsmentioning

confidence: 99%

Genotype to phenotype: Diet-by-mitochondrial DNA haplotype interactions drive metabolic flexibility and organismal fitness

Towarnicki

Melvin

et al. 2018

PLoS Genet

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Diet may be modified seasonally or by biogeographic, demographic or cultural shifts. It can differentially influence mitochondrial bioenergetics, retrograde signalling to the nuclear genome, and anterograde signalling to mitochondria. All these interactions have the potential to alter the frequencies of mtDNA haplotypes (mitotypes) in nature and may impact human health. In a model laboratory system, we fed four diets varying in Protein: Carbohydrate (P:C) ratio (1:2, 1:4, 1:8 and 1:16 P:C) to four homoplasmic Drosophila melanogaster mitotypes (nuclear genome standardised) and assayed their frequency in population cages. When fed a high protein 1:2 P:C diet, the frequency of flies harbouring Alstonville mtDNA increased. In contrast, when fed the high carbohydrate 1:16 P:C food the incidence of flies harbouring Dahomey mtDNA increased. This result, driven by differences in larval development, was generalisable to the replacement of the laboratory diet with fruits having high and low P:C ratios, perturbation of the nuclear genome and changes to the microbiome. Structural modelling and cellular assays suggested a V161L mutation in the ND4 subunit of complex I of Dahomey mtDNA was mildly deleterious, reduced mitochondrial functions, increased oxidative stress and resulted in an increase in larval development time on the 1:2 P:C diet. The 1:16 P:C diet triggered a cascade of changes in both mitotypes. In Dahomey larvae, increased feeding fuelled increased β-oxidation and the partial bypass of the complex I mutation. Conversely, Alstonville larvae upregulated genes involved with oxidative phosphorylation, increased glycogen metabolism and they were more physically active. We hypothesise that the increased physical activity diverted energy from growth and cell division and thereby slowed development. These data further question the use of mtDNA as an assumed neutral marker in evolutionary and population genetic studies. Moreover, if humans respond similarly, we posit that individuals with specific mtDNA variations may differentially metabolise carbohydrates, which has implications for a variety of diseases including cardiovascular disease, obesity, and perhaps Parkinson’s Disease.

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Section: Resultsmentioning

confidence: 99%

Genotype to phenotype: Diet-by-mitochondrial DNA haplotype interactions drive metabolic flexibility and organismal fitness

Towarnicki

Melvin

et al. 2018

PLoS Genet

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“…In this paper, we elucidate the larval pupariation process by analyzing the transcriptome of B. dorsalis using RNA-seq at three developmental stages including the wandering stage (WS), late wandering stage (LWS, the body contraction just before pupariation), and white puparium stage (WPS). The use of RNA-seq is already well established for the analysis of gene expression in B. dorsalis [ 3 , 19 , 20 ], and the availability of a well annotated genome of B. dorsalis (NCBI Assembly: ASM78921v2), allowed us to identify the genes differentially expressed during the different developmental stages. The results obtained through the RNA-seq analysis were further confirmed by real-time quantitative PCR.…”

Section: Introductionmentioning

confidence: 99%

RNA-seq analysis of gene expression changes during pupariation in Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

et al. 2018

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BackgroundThe oriental fruit fly, Bactrocera dorsalis (Hendel) has been considered to be one of the most important agricultural pest around the world. As a holometabolous insect, larvae must go through a metamorphosis process with dramatic morphological and structural changes to complete their development. To better understand the molecular mechanisms of these changes, RNA-seq of B. dorsalis from wandering stage (WS), late wandering stage (LWS) and white puparium stage (WPS) were performed.ResultsIn total, 11,721 transcripts were obtained, out of which 1914 genes (578 up-regulated and 1336 down-regulated) and 2047 genes (655 up-regulated and 1392 down-regulated) were found to be differentially expressed between WS and LWS, as well as between WS and WPS, respectively. Of these DEGs, 1862 and 1996 genes were successfully annotated in various databases. The analysis of RNA-seq data together with qRT-PCR validation indicated that during this transition, the genes in the oxidative phosphorylation pathway, and genes encoding P450s, serine protease inhibitor, and cuticular proteins were down-regulated, while the serine protease genes were up-regulated. Moreover, we found some 20-hydroxyecdysone (20E) biosynthesis and signaling pathway genes had a higher expression in the WS, while the genes responsible for juvenile hormone (JH) synthesis, degradation, signaling and transporter pathways were down-regulated, suggesting these genes might be involved in the process of larval pupariation in B. dorsalis. For the chitinolytic enzymes, the genes encoding chitinases (chitinase 2, chitinase 5, chitinase 8, and chitinase 10) and chitin deacetylase might play the crucial role in the degradation of insect chitin with their expressions significantly increased during the transition. Here, we also found that chitin synthase 1A might be involved in the chitin synthesis of cuticles during the metamorphosis in B. dorsalis.ConclusionsSignificant changes at transcriptional level were identified during the larval pupariation of B. dorsalis. Importantly, we also obtained a vast quantity of RNA-seq data and identified metamorphosis associated genes, which would all help us to better understand the molecular mechanism of metamorphosis process in B. dorsalis.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5077-z) contains supplementary material, which is available to authorized users.

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“…In contrast, the similar annotations of DEGs in A and B groups are enriched in folate biosynthesis and lysosome pathways ( Table 4 ). Other studies, including one for B. dorsalis ( Chen et al 2017 ), have speculated about different genes and biochemical pathways may be expressed differentially in response to different diets.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of Novel Host Use by Bactrocera tau (Tephritid: Diptera) Revealed by RNA Transcriptomes

Shi

Roderick

Zhang

2020

Journal of Insect Science

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Use of novel plant hosts can facilitate the establishment and range expansion of herbivorous invasive species. However, the inherent mechanisms of novel host use are still unclear in many herbivorous species. Here, we examine mechanisms of novel host use in the invasive tephritid fruit fly Bactrocera tau (Walker)(Diptera: Tephritidae) by documenting changes in the RNA transcriptomes associated with a novel host. RNA transcripts of B. tau were obtained with high-throughput sequencing from samples continuously reared on two traditional Cucurbitaceae hosts and a novel host (banana). We found transcriptome variation was strongly associated with feeding on banana. Moreover, B. tau feeding on banana contained more differentially expressed genes (DEGs) and more annotated categories of DEGs in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database with 1,595 DEGs and 21 major annotated pathways. The annotated categories of DEGs in individuals reared on banana differed with from those individuals feeding on other hosts and were enriched in oxidative phosphorylation, citrate cycle pathway, and four other carbohydrate pathways. For B. tau feeding on banana, the predominant numbers of upregulated genes in the mitochondrial NADH (56 on average) and a relatively higher numbers of upregulated genes (13 on average) were found in oxidative phosphorylation and the TCA pathway, respectively. Changes in RNA transcriptomes associated with novel host use, especially for genes related to energy and carbohydrate metabolism, help to explain how B. tau can be successful in use of novel hosts and may be useful in developing novel strategies for control of tephritid flies.

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Phenotypes, antioxidant responses, and gene expression changes accompanying a sugar-only diet in Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Cited by 15 publications

References 75 publications

Genotype to phenotype: Diet-by-mitochondrial DNA haplotype interactions drive metabolic flexibility and organismal fitness

Genotype to phenotype: Diet-by-mitochondrial DNA haplotype interactions drive metabolic flexibility and organismal fitness

RNA-seq analysis of gene expression changes during pupariation in Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Mechanisms of Novel Host Use by Bactrocera tau (Tephritid: Diptera) Revealed by RNA Transcriptomes

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