BODIPY-Based Dye for No-Wash Live-Cell Staining and Imaging

Пахомов, А. А.; Deyev, I. E.; Ratnikova, Natalia M.; Чумаков, С. П.; Mironiuk, Veronika B.; Kononevich, Yuriy N.; Muzafarov, А. М.; Martynov, Vladimir I.

doi:10.2144/000114577

Cited by 17 publications

(17 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To further explore the dysfunction of fragmented mitochondria, we also examined lipid accumulation in marf ‐KD GSCs by TEM and a lipophilic dye (BODIPY 493/503 staining), as fatty acid oxidation takes place in mitochondria (Pakhomov et al, 2017). We observed a clear increase in number and size of oil droplets in marf ‐KD GSCs as compared to control or drp1 ‐KD GSCs one and 2 weeks after eclosion (Figure 5c–h,l, and Figure ), similar to previous observations in male GSCs (Sênos Demarco, Uyemura, D’Alterio, & Jones, 2019).…”

Section: Resultsmentioning

confidence: 99%

Aging shifts mitochondrial dynamics toward fission to promote germline stem cell loss

et al. 2020

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Aging shifts mitochondrial dynamics toward fission to promote germline stem cell loss

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Another important feature of HSC, earlier known as fat-storing cells, is the capacity to take up and accumulate lipids in lipid droplets. These intracellular, fat-storing organelles serving as neutral lipid reservoirs and the center of lipid metabolism can be easily colored by fluorescent dyes interacting with extremely hydrophobic substances and visualized by fluorescence microscopy including Nile Red or by different BODIPY-based dyes [ 20 , 21 ]. In particular, HSCs cultured in the presence of oleic acid form large intracellular lipid droplets and increase the expression of lipid-droplet proteins [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

Rat Hepatic Stellate Cell Line CFSC-2G: Genetic Markers and Short Tandem Repeat Profile Useful for Cell Line Authentication

Nanda

Schröder

Steinlein

et al. 2022

Cells

View full text Add to dashboard Cite

Hepatic stellate cells (HSCs) are also known as lipocytes, fat-storing cells, perisinusoidal cells, or Ito cells. These liver-specific mesenchymal cells represent about 5% to 8% of all liver cells, playing a key role in maintaining the microenvironment of the hepatic sinusoid. Upon chronic liver injury or in primary culture, these cells become activated and transdifferentiate into a contractile phenotype, i.e., the myofibroblast, capable of producing and secreting large quantities of extracellular matrix compounds. Based on their central role in the initiation and progression of chronic liver diseases, cultured HSCs are valuable in vitro tools to study molecular and cellular aspects of liver diseases. However, the isolation of these cells requires special equipment, trained personnel, and in some cases needs approval from respective authorities. To overcome these limitations, several immortalized HSC lines were established. One of these cell lines is CFSC, which was originally established from cirrhotic rat livers induced by carbon tetrachloride. First introduced in 1991, this cell line and derivatives thereof (i.e., CFSC-2G, CFSC-3H, CFSC-5H, and CFSC-8B) are now used in many laboratories as an established in vitro HSC model. We here describe molecular features that are suitable for cell authentication. Importantly, chromosome banding and multicolor spectral karyotyping (SKY) analysis demonstrate that the CFSC-2G genome has accumulated extensive chromosome rearrangements and most chromosomes exist in multiple copies producing a pseudo-triploid karyotype. Furthermore, our study documents a defined short tandem repeat (STR) profile including 31 species-specific markers, and a list of genes expressed in CFSC-2G established by bulk mRNA next-generation sequencing (NGS).

show abstract

“…For example, lipophilic cations like rhodamine will localise in the mitochondria, while lipophilic BODIPYs will tend to accumulate in lipid droplets. 178 A further confounding factor is the tendency of some fluorophores to display varying brightness in different environments, such as the solvatochromic or fluorogenic behaviour of lipid stains in non-polar environments, or the Please do not adjust margins Please do not adjust margins sensitivity of some fluorophores to photoinduced electron transfer quenching in different pH environments. 179,180 As these effects can give the impression of successful targeting, it is important to validate targeting groups with non-responsive fluorophores as controls.…”

Section: Trends In Organelle Targetingmentioning

confidence: 99%