In vitro expression of Sec-dependent pathway and type 4B secretion system in Piscirickettsia salmonis

Cortés, Marcos; Sánchez, Patricio; Ruiz, Pamela; Haro, Ronie E.; Sáez, Jerson; Sánchez, F Velasco; Hernández, Mauricio; Oliver, Cristian; Yáñez, Alejandro J.

doi:10.1016/j.micpath.2017.08.003

Cited by 14 publications

(12 citation statements)

References 39 publications

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“…Additionally, the Sec‐dependent pathway and the type 4B secretion systems were found to be biologically active during P. salmonis infection (Cortés et al . ).…”

Section: Piscirickettsia Salmonismentioning

confidence: 97%

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Figueroa

Cárcamo

Yáñez

et al. 2019

Reviews in Aquaculture

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The rapid growth of the salmon farming industry in Chile has led to the appearance of various viral, bacterial, parasitic, and fungal pathogens affecting farmed fish. The Chilean salmon industry has suffered several health crises associated with high fish mortalities, such as caused by the infectious salmon anaemia virus (2007) and harmful algal blooms (2016). In addition to these events, marine farms are continuously affected by outbreaks of harmful pathogens, including the bacteria Piscirickettsia salmonis and, most recently, a reappearance of Renibacterium salmoninarum, and the infectious pancreatic necrosis virus. These outbreaks have led to stricter regulations, but the salmon farming industry continues to struggle despite reforms. In addition to the aforementioned pathogens, which are a continuous threat, other apparently under control pathogens have also reappeared in recent years. In this review, we analyse the current state of knowledge on four of the main pathogens affecting salmon farming in Chile. The infectious pancreatic necrosis virus is relevant as it affects freshwater‐stage fish, with survivors then acting as carriers. The infectious salmon anaemia virus currently appears to be under control; however, P. salmonis and R. salmoninarum continue to be the cause for high mortalities in the Chilean aquaculture industry.

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“…Additionally, the Sec‐dependent pathway and the type 4B secretion systems were found to be biologically active during P. salmonis infection (Cortés et al . ).…”

Section: Piscirickettsia Salmonismentioning

confidence: 97%

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Figueroa

Cárcamo

Yáñez

et al. 2019

Reviews in Aquaculture

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“…The genome sequence of different strains of P. salmonis indicates the presence of two or three of dot<underline/>icm gene clusters encoding the type IVB secretion system (Bohle et al, 2014;Pulgar et al, 2015b). P. salmonis Dot/Icm is homologous to the T4BSS of the Gram negative intracellular pathogens Legionella pneumophila and Coxiella burnetii (Gómez et al, 2013;Cortés et al, 2017). For these two intracellular pathogens, it has been demonstrated that effector proteins translocated by the T4BSS modulate host cell pathways to enable their intracellular replication and survival in the host cell.…”

Section: Infection Modelmentioning

confidence: 99%

“…For these two intracellular pathogens, it has been demonstrated that effector proteins translocated by the T4BSS modulate host cell pathways to enable their intracellular replication and survival in the host cell. In the case of P. salmonis, the transcripts and proteins of several structural components of the T4BSS have been detected in P. salmonis-infected cells (Gómez et al, 2013;Cortés et al, 2017). Moreover, a mutation in a structural component of P. salmonis T4BSS leads to a reduced virulence in vitro and a decreased mortality in an in vivo assay (Mancilla et al, 2018), suggesting that the Dot/Icm system may be essential for P. salmonis intracellular replication and survival.…”

Section: Infection Modelmentioning

confidence: 99%

“…We noticed that genes encoding several Dot/Icm proteins were differentially expressed during broth and intracellular growth. Early evidence from P. salmonis LF89 genome sequencing revealed putative gene products with high amino acid homology to Dot/Icm proteins of L. pneumophila and C. burnetii (Gómez et al, 2013;Pulgar et al, 2015b;Cortés et al, 2017). The majority of P. salmonis dot/icm genes were found on three regions of the chromosome, with the icmF and dotU genes lying outside of three main regions.…”

Section: Gene Expression Pattern Of Predicted Dot/icm Proteins Of P mentioning

confidence: 99%

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Transcriptomic Changes of Piscirickettsia salmonis During Intracellular Growth in a Salmon Macrophage-Like Cell Line

Zúñiga

Aravena

Pulgar

et al. 2020

Front. Cell. Infect. Microbiol.

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“…In this context, genes closely related to the Dot/Icm system found in Legionella were previously identified in the genome of the P. salmonis LF-89 strain (Eppinger et al, 2013). Consistent with their putative pathogenic role, the transcripts for genes encoding several T4SS components in P. salmonis-infected cell cultures have been detected, and evidence of their protein translation was also recently added (Gomez et al, 2013;Cortes et al, 2017). Moreover, some putative T4SS effectors have been identified bioinformatically (Labra, Arredondo-Zelada, Flores-Herrera, Marshall, & Gomez, 2016).…”

Section: Gram-negative Pathogens Coxiella and Legionella That Are Relmentioning

confidence: 78%

The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar

Mancilla¹,

Saavedra²,

Grandón³

et al. 2017

Journal of Fish Diseases

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Piscirickettsiosis is a threatening infectious disease for the salmon industry, due to it being responsible for significant economic losses. The control of outbreaks also poses considerable environmental challenges. Despite Piscirickettsia salmonis having been discovered as the aetiological agent of the disease more than 25 years ago, its pathogenicity remains poorly understood. Among virulence factors identified so far, type four secretion systems (T4SS) seem to play a key role during the infection caused by the bacterium. We report here the genetic manipulation of P. salmonis by means of the transference of plasmid DNA in mating assays. An insertion cassette was engineered for targeting the icmB gene, which encodes a putative T4SS‐ATPase and is carried by one of the chromosomal T4SS clusters found within the genome of P. salmonis PM15972A1, a virulent representative of the EM‐90‐like strain. The molecular characterization of the resulting mutant strain demonstrated that the insertion interrupted the target gene. Further in vitro testing of the icmB mutant showed a dramatic drop in infectivity as tested in CHSE‐214 cells, which is in agreement with its attenuated behaviour observed in vivo. Altogether, our results demonstrate that, similar to other facultative intracellular pathogens, P. salmonis’ virulence relies on an intact T4SS.

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In vitro expression of Sec-dependent pathway and type 4B secretion system in Piscirickettsia salmonis

Cited by 14 publications

References 39 publications

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Transcriptomic Changes of Piscirickettsia salmonis During Intracellular Growth in a Salmon Macrophage-Like Cell Line

The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar

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