Dysbiotic Proteobacteria expansion: a microbial signature of epithelial dysfunction

Litvak, Yael; Byndloss, Mariana X.; Tsolis, Renée M.; Bäumler, Andreas J.

doi:10.1016/j.mib.2017.07.003

Cited by 416 publications

(278 citation statements)

References 49 publications

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“…Moreover, the LEfSe results showed that Enterobacteriaceae belonging to the phylum Proteobacteria were over-represented in the IIBV group. Previous research shows that in the case of intestinal inflammation, epithelial cells reduce their capacity to undergo beta-oxidation, with the consequence of an increased availability of oxygen, which is thought to promote the bloom of Proteobacteria and the dysbiosis of the gut microbiota 46,47 . Further investigations acknowledged that nitrate produced by the host during inflammatory conditions can be exploited by commensal Enterobacteriaceae, which thus become predominant 48 .…”

Section: Discussionmentioning

confidence: 99%

16S rRNA gene sequencing reveals an altered composition of the gut microbiota in chickens infected with a nephropathogenic infectious bronchitis virus

Shi

Liu

et al. 2020

Sci Rep

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Infectious bronchitis virus (IBV), a member of the Coronaviridae family, causes serious losses to the poultry industry. Intestinal microbiota play an important role in chicken health and contribute to the defence against colonization by invading pathogens. The aim of this study was to investigate the link between the intestinal microbiome and nephropathogenic IBV (NIBV) infection. Initially, chickenswere randomly distributed into 2 groups: the normal group (INC) and the infected group (IIBV). The ilea were collected for morphological assessment, and the ileal contents were collected for 16S rRNA gene sequencing analysis. The results of the IIBV group analyses showed a significant decrease in the ratio of villus height to crypt depth (P < 0.05), while the goblet cells increased compared to those in the INC group. Furthermore, the microbial diversity in the ilea decreased and overrepresentation of Enterobacteriaceae and underrepresentation of Chloroplast and Clostridia was found in the NIBVinfected chickens. In conclusion, these results showed that the significant separation of the two groups and the characterization of the gut microbiome profiles of the chickens with NIBV infection may provide valuable information and promising biomarkers for the diagnosis of this disease.

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Section: Discussionmentioning

confidence: 99%

16S rRNA gene sequencing reveals an altered composition of the gut microbiota in chickens infected with a nephropathogenic infectious bronchitis virus

Shi

Liu

et al. 2020

Sci Rep

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“…The resultant dysanaerobiosis, an increase of oxygen that disrupts anaerobic environment in the gut, would enable selection for facultative anaerobes, including commensal and pathogenic E. coli by aerobic respiration. In this study, florfenicol treatment caused an abnormal escalation of Proteobacteria (mainly E. coli), resulting in dysbiosis of the gut microbial community which can have a number of consequences as it enhances the risk of disease (Shin et al, 2015;Litvak et al, 2017). Escherichia spp continually produce ethanol which increases the permeability of the intestinal wall and promotes colonization by obligate aerobes, characteristics linked to inflammation and obesity.…”

Section: Discussionmentioning

confidence: 91%

Preventive antibiotic treatment of calves: emergence of dysbiosis causing propagation of obese state‐associated and mobile multidrug resistance‐carrying bacteria

Dobrzanska

Lamaudière

Rollason

et al. 2019

Microbial Biotechnology

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Summary In agriculture, antibiotics are used for the treatment and prevention of livestock disease. Antibiotics perturb the bacterial gut composition but the extent of these changes and potential consequences for animal and human health is still debated. Six calves were housed in a controlled environment. Three animals received an injection of the antibiotic florfenicol (Nuflor), and three received no treatment. Faecal samples were collected at 0, 3 and 7 days, and bacterial communities were profiled to assess the impact of a therapy on the gut microbiota. Phylogenetic analysis (16S‐rDNA) established that at day 7, antibiotic‐treated microbiota showed a 10‐fold increase in facultative anaerobic Escherichia spp, a signature of imbalanced microbiota, dysbiosis. The antibiotic resistome showed a high background of antibiotic resistance genes, which did not significantly change in response to florfenicol. However, the maintenance of Escherichia coli plasmid‐encoded quinolone, oqxB and propagation of mcr‐2, and colistin resistance genes were observed and confirmed by Sanger sequencing. The microbiota of treated animals was enriched with energy harvesting bacteria, common to obese microbial communities. We propose that antibiotic treatment of healthy animals leads to unbalanced, disease‐ and obese‐related microbiota that promotes growth of E. coli carrying resistance genes on mobile elements, potentially increasing the risk of transmission of antibiotic resistant bacteria to humans.

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“…1BC). This circumstance is distinct from that that 1 8 7 occur in the mature gut, where low O 2 is linked to a healthy condition and aerobicity is 1 8 8 linked to inflammation (Litvak et al, 2017). gut lumen, this is probably the main source of NO in the gut lumen (Singer et al, 1996).…”

Section: 4mentioning

confidence: 93%

Shifts in microbial transcription patterns prior to onset of Necrotizing enterocolitis may indicate low oxygen levels in the gut

Sher

Raveh-Sadka

et al. 2018

Preprint

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SummaryPremature infants are at risk for developing necrotizing enterocolitis (NEC), an inflammatory disease that can progress to necrosis of gut tissue. Previous attempts have failed to identify any consistent predictor of NEC. We hypothesized that prior to the appearance of NEC symptoms, the gut microbiome shifts in its transcriptional profile. To test this hypothesis we integrated genome-resolved metagenomic and metatranscriptomic data from multiple time-points in the first month of life of four preterm infants, two of whom later developed NEC. Gut microbiomes of NEC infants showed increased transcription of high oxygen affinity cytochrome oxidases and lower transcription of genes to detoxify nitric oxide, an antimicrobial compound released by host cells. These results, and high transcription of H2 production genes, suggest low O2 conditions prior to NEC onset, and are consistent with hypoxic conditions in diseased gut tissue. The findings motivate further testing of transcript data as a predictor of NEC.HighlightsTranscription of high oxygen affinity microbial cytochrome oxidase may predict necrotizing enterocolitis (NEC) development.Lower transcription of microbial genes to detoxify nitric oxide (NO) may also predict NEC development.Higher transcription of H2 production genes by Escherichia sp. was found in the gut of premature infants that develop NEC.

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Dysbiotic Proteobacteria expansion: a microbial signature of epithelial dysfunction

Cited by 416 publications

References 49 publications

16S rRNA gene sequencing reveals an altered composition of the gut microbiota in chickens infected with a nephropathogenic infectious bronchitis virus

16S rRNA gene sequencing reveals an altered composition of the gut microbiota in chickens infected with a nephropathogenic infectious bronchitis virus

Preventive antibiotic treatment of calves: emergence of dysbiosis causing propagation of obese state‐associated and mobile multidrug resistance‐carrying bacteria

Shifts in microbial transcription patterns prior to onset of Necrotizing enterocolitis may indicate low oxygen levels in the gut

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