HIV virions sense plasma membrane heterogeneity for cell entry

Yang, Seun-Ah; Kreutzberger, Alex J. B.; Kiessling, Volker; Ganser‐Pornillos, Barbie K.; White, Judith M.; Tamm, Lukas K.

doi:10.1126/sciadv.1700338

Cited by 100 publications

(127 citation statements)

References 47 publications

(62 reference statements)

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“…For example, single particle binding studies have revealed the importance of microdomains in binding of norovirus-like particles, which preferentially bind the edges of glycosphingolipid-enriched domains [49]. Later it was also shown that HIV particles prefer to bind at the edges of cholesterol-rich lipid domains that were reconstituted in supported bilayers [86,87]; however, observing this preference in live cells due to the small scale and dynamism of lipid rafts is difficult, illustrating the power of using an in vitro system for such studies. Focusing further on the receptors themselves, TIRF microscopy has also been used to measure the affinity of HIV glycoprotein 120 for the glycosphingolipids galactosyl ceramide, glucosylceramide, lactosylceramide and α-hydroxy glucosylceramide in SLBs [88,89].…”

Section: Single Virion Tracking Of Bindingmentioning

confidence: 99%

Single Virion Tracking Microscopy for the Study of Virus Entry Processes in Live Cells and Biomimetic Platforms

Nathan

Daniel

2019

Advances in Experimental Medicine and Biology

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The most widely-used assays for studying viral entry, including infectivity, cofloatation, and cell-cell fusion assays, yield functional information but provide low resolution of individual entry steps. Structural characterization provides highresolution conformational information, but on its own is unable to address the functional significance of these conformations. Single virion tracking microscopy techniques provide more detail on the intermediate entry steps than infection assays and more functional information than structural methods, bridging the gap between these methods. In addition, single virion approaches also provide dynamic information about the kinetics of entry processes. This chapter reviews single virion tracking techniques and describes how they can be applied to study specific virus entry steps. These techniques provide information complementary to traditional ensemble approaches. Single virion techniques may either probe virion behavior in live cells or in biomimetic platforms. Synthesizing information from ensemble, structural, and single virion techniques ultimately yields a more complete understanding of the viral entry process than can be achieved by any single method alone.

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Section: Single Virion Tracking Of Bindingmentioning

confidence: 99%

Single Virion Tracking Microscopy for the Study of Virus Entry Processes in Live Cells and Biomimetic Platforms

Nathan

Daniel

2019

Advances in Experimental Medicine and Biology

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“…Based on the manner of illumination, TIRFM setups can be categorized as wide‐field or point‐scanning . The general wide‐field TIRFM setup ensures high temporal resolution, and therefore, it is adopted more often and widely used to study the dynamic molecular behaviors of plasma membrane‐bound proteins or labeled biomolecules on a surface in vivo, e.g., for investigations of vesicle endocytosis and exocytosis, plasma membrane heterogeneity, cell growth, protein stoichiometry, neuron structure, and T lymphocyte adhesion . These applications are vital for understanding the structures and functions of biological samples, because many basic cellular processes are performed by complex biological mechanisms in the cell plasma membrane.…”

Section: Methods For Axial Srfmmentioning

confidence: 99%

Breaking the Axial Diffraction Limit: A Guide to Axial Super‐Resolution Fluorescence Microscopy

Liu

Toussaint

Okoro

et al. 2018

Laser & Photonics Reviews

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Optical microscopy is a powerful tool for understanding the fundamentals of the microscopic world. However, for centuries its resolving ability remained limited by the optical diffraction limit. Super‐resolution fluorescence microscopy (SRFM) has been introduced to break the diffraction limit and significantly expand the fields in which optical microscopy can be applied. Unfortunately, SRFM contributes little towards axial resolution enhancement, rendering observation of the axial and three‐dimensional structures of biological tissues difficult; this may yield a misunderstanding of intracellular interactions. Based on the existing literature, the development of axial SRFM is still behind that of lateral SRFM. In light of this, this Review presents a comprehensive summary of the principles, development, characteristics, and applications of existing techniques for improving the axial resolution. This Review will provide a guide to researchers and promote further development of related technology.

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“…We next explored the extent to which membrane domains could enhance membrane fusion between phospholipid vesicles.Recent studies with model membranes and computational studies have shown that the presence of phasesegregated domains in lipid vesicles could enhance fusion induced by an HIV fusion peptide. [22,26,28,42] These studies demonstrated that membrane phase heterogeneity and line tension are important for viral membrane fusion. We wondered if membrane domains could be similarly used to enhance DNA-mediated vesicle fusion.…”

Section: Membrane Domains Promote Dna-mediated Membrane Fusionmentioning

confidence: 99%

Barcoding Biological Reactions with DNA‐Functionalized Vesicles

Peruzzi

Jacobs

et al. 2019

Angew Chem Int Ed

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Targeted vesicle fusion is a promising approach to selectively control interactions between vesicle compartments and would enable the initiation of biological reactions in complex aqueous environments. Here, we explore how two features of vesicle membranes, DNA tethers and phase‐segregated membranes, promote fusion between specific vesicle populations. Membrane phase‐segregation provides an energetic driver for membrane fusion that increases the efficiency of DNA‐mediated fusion events. The orthogonality provided by DNA tethers allows us to direct fusion and delivery of DNA cargo to specific vesicle populations. Vesicle fusion between DNA‐tethered vesicles can be used to initiate in vitro protein expression to produce model soluble and membrane proteins. Engineering orthogonal fusion events between DNA‐tethered vesicles provides a new strategy to control the spatiotemporal dynamics of cell‐free reactions, expanding opportunities to engineer artificial cellular systems.

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HIV virions sense plasma membrane heterogeneity for cell entry

Abstract: HIV virions target co-receptors and fuse at ordered/disordered domain boundaries in cholesterol-rich plasma membranes.

Cited by 100 publications

References 47 publications

Single Virion Tracking Microscopy for the Study of Virus Entry Processes in Live Cells and Biomimetic Platforms

Single Virion Tracking Microscopy for the Study of Virus Entry Processes in Live Cells and Biomimetic Platforms

Breaking the Axial Diffraction Limit: A Guide to Axial Super‐Resolution Fluorescence Microscopy

Barcoding Biological Reactions with DNA‐Functionalized Vesicles

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