Response to manuscript by Pfaff et al.: Evidence against a role of DJ-1 in methylglyoxal detoxification

Reply to Richarme: Evidence against a role of DJ-1 in methylglyoxal detoxification

“…The authors declare that they have no conflicts of interest with the contents of this article. 1 To whom correspondence may be addressed. Tel.…”

“…This is a response to a letter by Richarme (1). We thank Dr. Richarme for the opportunity to clarify the points raised in his letter.…”

Kinetic evidence in favor of glyoxalase III and against deglycase activity of DJ‐1

“…Later, it was also reported that DJ‐1 and its bacterial homologs (Hsp31, YhbO, and YajL) specifically repair the reversible glycated guanosine 5′‐triphosphate (GTP aminocarbinol), but not its delayed end‐products, namely imidazopurinones and carboxyethyl‐deoxyguanosine (Richarme et al, 2017 ). Nevertheless, there is still a debate regarding the deglycase activity of DJ‐1 including potential TRIS buffer artifacts and the D/L‐stereochemistry of the lactate products (Pfaff et al, 2017 ; Richarme, 2017 ). It was recently reported that the apparent deglycase activity of DJ‐1 is the consequence of fast equilibrium between MG and hemithioacetals, likely resulting in the misinterpretation of glyoxalase III as deglycase (Andreeva et al, 2019 ; Gao et al, 2023 ; Mazza et al, 2022 ).…”