2-Dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione inhibits the growth and metastasis of breast carcinoma in mice

Chen, Chunxia; Nong, Zhihuan; Xie, Qiuqiao; He, Junhui; Wene, Cai; Tang, Xiuneng; Chen, Xiaoyu; Huang, Renbin; Gao, Ying

doi:10.1038/s41598-017-07162-3

Cited by 25 publications

(23 citation statements)

References 37 publications

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“…Therefore, the H&E staining images of representative sections of lungs are illustrated (Figure 5C). Typical tumor nodules were observed in the saline group, visualizing the metastasized cancer cells and their localization, as reported previously 35,36. Smaller and fewer nodules were found in lungs with IFN‐γ and NP‐TOS15 treatment, indicating the lung metastasis was inhibited to some degree.…”

Section: Resultssupporting

confidence: 83%

Enhanced Prevention of Breast Tumor Metastasis by Nanoparticle‐Delivered Vitamin E in Combination with Interferon‐Gamma

Liu

Movahedi

et al. 2020

Adv Healthcare Materials

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Preventing cancer metastasis is one of the remaining challenges in cancer therapy. As an efficient natural product, alpha‐tocopheryl succinate (α‐TOS), the most effective form of vitamin E, holds great anticancer potential. To improve its efficacy and bioavailability, lipid‐coated calcium carbonate/phosphate (LCCP) nanoparticles (NPs) with folic acid and PEG modification are synthesized for efficient delivery of α‐TOS to 4T1 cancer cells. The optimized LCCP‐FA NPs (NP‐TOS15) show an α‐TOS loading efficiency of around 60%, and enhanced uptake by 4T1 metastatic cancer cells. Consequently, NP‐TOS15 significantly enhance the anticancer effect in combination with interferon‐gamma (IFN‐γ) in terms of apoptosis facilitation and migration inhibition. Importantly, NP‐TOS15 upregulate the anticancer immunity via downregulating program death ligand 1 (PD‐L1) expression that is initially induced by IFN‐γ, and remarkably prevent the lung metastasis, particularly in combination with IFN‐γ. Further investigation reveals that this combination therapy also modulates the cytotoxic lymphocyte infiltration into the tumor microenvironment for tumor elimination. Taken together, the NP delivery of α‐TOS in combination with IFN‐γ provides an applicable strategy for cancer therapy.

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Section: Resultssupporting

confidence: 83%

Enhanced Prevention of Breast Tumor Metastasis by Nanoparticle‐Delivered Vitamin E in Combination with Interferon‐Gamma

Liu

Movahedi

et al. 2020

Adv Healthcare Materials

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“…Immunohistochemical procedures were conducted according to our previous study [ 52 ]. Briefly, the deparaffinized and hydrated slides were incubated in 3 % H 2 O 2 for 30 min to block the endogenous peroxidase activity and were later placed in citrate buffer (PH = 6) for antigen retrieval.…”

Section: Methodsmentioning

confidence: 99%

Hyperbaric oxygen protects against myocardial reperfusion injury via the inhibition of inflammation and the modulation of autophagy

Chen

et al. 2017

Oncotarget

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Our previous study demonstrated that hyperbaric oxygen (HBO) preconditioning protected against myocardial ischemia reperfusion injury (MIRI) and improved myocardial infarction. However, HBO’s effect on MIRI-induced inflammation and autophagy remains unclear. In this study, we investigate the potential impact and underlying mechanism of HBO preconditioning on an MIRI-induced inflammatory response and autophagy using a ligation of the left anterior descending (LAD) coronary artery rat model. Our results showed that HBO restored myocardial enzyme levels and decreased the apoptosis of cardiomyocytes, which were induced by MIRI. Moreover, HBO significantly suppressed MIRI-induced inflammatory cytokines. This effect was associated with the inhibition of the TLR4-nuclear factor kappa-B (NF-κB) pathway. Interestingly, lower expression levels of microtubule-associated protein 1 light chain 3B (LC3B) and Beclin-1 were observed in the HBO-treatment group. Furthermore, we observed that HBO reduced excessive autophagy by activating the mammalian target of the rapamycin (mTOR) pathway, as evidenced by higher expression levels of threonine protein kinase (Akt) and phosphorylated-mTOR. In conclusion, HBO protected cardiomocytes during MIRI by attenuating inflammation and autophagy. Our results provide a new mechanistic insight into the cardioprotective role of HBO against MIRI.

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“…This study uses a high-power infrared laser (at 1064 nm) to measure the radio sensitivity of 4T1 breast carcinoma cell lines treated by a naturally occurring compound, 2-Dodecyl-6-methoxycyclohexa-2, 5-diene-1, 4-dione (DMDD) extracted from the root of Averrhoa carambola L. Studies have shown that DMDD induces apoptosis of various human breast cancer cells through production of intracellular reactive oxygen species (ROS) and inhibition of NF-κB activation 21 . Recent studies, using an in vivo mouse model of transplanted 4T1 breast cancer cells, have also shown that DMDD effectively suppressed the growth of primary breast tumor and simultaneously inhibited the metastasis of breast tumor to the lung and liver, as well as prolonged the survival of tumor-bearing mice 22 . The 4T1 breast carcinoma cell line is highly tumorigenic and invasive; it can metastasize from the primary tumor to multiple distant sites such as lymph nodes, liver, lung, brain, and bone 22 .…”

Section: Introductionmentioning

confidence: 99%

Chemo-treated 4T1 breast cancer cells radiation response measured by single and multiple cell ionization using infrared laser trap

Muhammed

Chen

Gao

et al. 2019

Sci Rep

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We present a study that uses a laser trapping technique for measurement of radiation sensitivity of untreated and chemo-treated cancer cells. We used a human mammary tumor cell line (4T1) treated by an antitumor compound, 2-Dodecyl-6-methoxycyclohexa-2, 5-diene-1,4-dione (DMDD), which was extracted from the root of Averrhoa carambola L. The untreated control group, and both 2-hour and 24-hour treated groups of 4T1 cells were used in this study. The absorbed threshold ionization energy (TIE) and the threshold radiation dose (TRD) were determined using a high-power infrared laser (at 1064 nm) trap by single and multiple cells trapping and ionization. The results were analyzed using descriptive and t-statistics. The relation of the TIE and TRD to the mass of the individual cells were also analyzed for different hours of treatment in comparison with the control group. Both TIE and TRD decrease with increasing treatment periods. However, the TRD decreases with mass regardless of the treatment. Analyses of the TRD for single vs multiple cells ionizations within each group have also consistently showed this same behavior regardless of the treatment. The underlying factors for these observed relations are explained in terms of radiation, hyperthermia, and chemo effects.

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2-Dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione inhibits the growth and metastasis of breast carcinoma in mice

Cited by 25 publications

References 37 publications

Enhanced Prevention of Breast Tumor Metastasis by Nanoparticle‐Delivered Vitamin E in Combination with Interferon‐Gamma

Enhanced Prevention of Breast Tumor Metastasis by Nanoparticle‐Delivered Vitamin E in Combination with Interferon‐Gamma

Hyperbaric oxygen protects against myocardial reperfusion injury via the inhibition of inflammation and the modulation of autophagy

Chemo-treated 4T1 breast cancer cells radiation response measured by single and multiple cell ionization using infrared laser trap

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