Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis

Abstract

BackgroundWhen bacteria colony persist within a biofilm, suitable drugs are not yet available for the eradication of biofilm-producing bacteria. The aim of this study is to study the effect of magnetic nano-particles-induced hyperthermia on destroying biofilm and promoting bactericidal effects of antibiotics in the treatment of osteomyelitis.MethodsSixty 12-weeks-old male Wistar rats were used. A metallic 18G needle was implanted into the bone marrow cavity of distal femur after the injection of Methicillin-sensitive Staphylococcus aureus (MSSA). All animals were divided into 5 different treatment modalities. The microbiological evaluation, scanning electron microscope examination, radiographic examination and then micro-CT evaluation of peri-implant bone resorption were analyzed.ResultsThe pathomorphological characteristics of biofilm formation were completed after 40-days induction of osteomyelitis. The inserted implants can be heated upto 75 °C by magnetic heating without any significant thermal damage on the surrounding tissue. We also demonstrated that systemic administration of vancomycin [VC (i.m.)] could not eradicate the bacteria; but, local administration of vancomycin into the femoral canal and the presence of magnetic nanoparticles hyperthermia did enhance the eradication of bacteria in a biofilm-based colony. In these two groups, the percent bone volume (BV/TV: %) was significantly higher than that of the positive control.ConclusionsFor the treatment of chronic osteomyelitis, we developed a new modality to improve antibiotic efficacy; the protection effect of biofilms on bacteria could be destroyed by magnetic nanoparticles-induced hyperthermia and therapeutic effect of systemic antibiotics could be enhanced.

Synergy between induction heating, antibiotics, and N-acetylcysteine eradicates Staphylococcus aureus from biofilm

“…Chopra et al have shown, in a mouse model, that thermal damage is confined to a localized region (< 2 mm) around the implant [8]. Fang et al heated metal implants in a rat model to 75 C without any significant thermal damage to the surrounding tissue [31]. These studies are in agreement with Samara et al, who have shown that bone cement achieves durable fixation despite the temperature reaching 80 C for more than 10 min, caused by the curing process of the cement [14].…”

Nano-Based Drug Delivery or Targeting to Eradicate Bacteria for Infection Mitigation: A Review of Recent Advances

“…The intramuscular route showed a similar tendency to suppress S. aureus accumulation in bone. Osteomyelitis was also treated by magnetic Fe 3 O 4 nanoparticles with the induction of hyperthermia to disrupt biofilm (Fang et al, 2017). The SPIONs were implanted in bone infected by S. aureus.…”

Targeting feasibility evaluation of magnetic resonance-guided focused ultrasound in the management of osteomyelitis: a virtual treatment planning study in 75 patients

“…An in vivo study investigated the use of magnetic particles hyperthermia to destroy biofilms generated in peri-implant osteomyelitis [58]. A metallic needle was implanted into the bone marrow cavity of rats and heated to 75 for up to 30 s at a time using an alternated magnetic field.…”