Kiloniella antarctica sp. nov., isolated from a polynya of Amundsen Sea in Western Antarctic Sea

Abstract

A taxonomic study was conducted on strain soj2014T, which was isolated from the surface water of a polynya in the Antarctic Sea. Comparative 16S rRNA gene sequence analysis showed that strain soj2014T belongs to the family Kiloniellaceae and is closely related to Kiloniella spongiae MEBiC09566T, 'Kiloniella litopenaei' P1-1T and Kiloniella laminariae LD81T (98.0 %, 97.8 % and 96.2 % 16S rRNA gene sequence similarity, respectively). The DNA-DNA hybridization values between strain soj2014T and closely related strains were below 28.6 %. The G+C content of the genomic DNA of strain soj2014T was 45.5 mol%. The predominant cellular fatty acids were summed feature 8 (composed of C18 : 1ω6c/C18 : 1ω7c, 57.0 %) and summed feature 3 (composed of C16 : 1ω6c/C16 : 1ω7c, 23.5 %). Strain soj2014T was Gram-stain-negative, slightly curved, spiral-shaped, and motile with a single polar flagellum. The strain grew at 0-30 °C (optimum, 25 °C), in 1.5-5.1 % (w/v) NaCl (optimum, 2.1-2.4 %) and at pH 5.5-9.5 (optimum, 7.5-8.0). It also had differential carbohydrate utilization traits and enzyme activities compared with closely related strains. Based on these phylogenetic, phenotypic and chemotaxonomic analyses, strain soj2014T represents a distinct species, separable from the reference strains, and is, therefore, proposed as a novel species, Kiloniella antarctica sp. nov. The type strain is soj2014T (=KCTC 42186T=JCM 30386T).

Microbial communities associated with sediments and polymetallic nodules of the Peru Basin

“…Similarly, Kiloniellaceae related OTUs might be involved in metal-cycling as closely related sequences were found in marine basalts (Mason et al, 2007;Santelli et al, 2008) and inside other manganese nodules (Blöthe et al, 2015). Most of the marine cultivates in the family Kiloniellaceae belong to genus Kiloniella, that have been isolated from marine macroalga (Wiese et al, 2009), the guts of Pacific white shrimp (Wang et al, 2015), marine sponge (Yang et al, 2015), spider crab and clam (Gerpe et al, 2017), and from the surface water of a polynia in the Western Antarctic Sea (Si et al, 2017).…”

The contribution of microbial communities in polymetallic nodules to the diversity of the deep-sea microbiome of the Peru Basin (4130–4198 m depth)

“…1 × 10 6 km 2 , including all current exploration licences (Miller et al, 2018). The regrowth of nodules will take millions of years; thus it is unknown whether the associated biota could recover at all (Simon-Lledo et al, 2019). The response of microbial communities to the loss of nodules and sea floor integrity is largely unknown.…”