Functional characterization of BdB1, a well-conserved carboxylesterase among tephritid fruit flies associated with malathion resistance in Bactrocera dorsalis (Hendel)

Wang, Luoluo; Lu, Xue‐Ping; Smagghe, Guy; Meng, Liesu; Wang, Jinjun

doi:10.1016/j.cbpc.2017.07.001

Cited by 20 publications

(47 citation statements)

References 39 publications

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“…However, silencing LbEST4 shows lower mortality when the psocids were exposed to deltamethrin. Our result is consistent with a previous study that showed knockdown of esterase genes in Bactrocera dorsalis also increased the sensitivity of fruit flies to malathion (Wang et al, 2016(Wang et al, , 2017. In P. xylostella, decreased expression level of EST gene by RNAi could also significantly increase the sensitivity of larvae to the insecticide chlorpyrifos (Xie et al, 2017).…”

Section: Discussionsupporting

confidence: 93%

Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

et al. 2020

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Esterases (ESTs) play important roles in metabolizing various physiologically endogenous and exogenous compounds, and various environmental xenobiotics in insects. The psocid, Liposcelis bostrychophila is a major pest of stored products worldwide and rapidly develops resistance to commonly insecticides. However, the involvement of ESTs in insecticide metabolization and the application of RNAi approach in psocids have not been well elucidated. In this study, we characterized four LbEST genes and investigated the transcriptional levels of these genes at different developmental stages and under different insecticides exposures to assess their potential roles in response to insecticides. The four LbESTs contain a catalytic triad (Ser-His-Glu) linked to an oxyanion hole and acyl pocket involved in substrate stabilization during its hydrolysis. Synergism observed with the esterase-inhibitor DEF suggests the involvement of esterases in malathion detoxification. LbESTs were expressed during the whole of developmental stages, but predominant abundance in the first nymphal instar and adult stage. The mRNA level of three LbEST genes (except for LbEST4) was induced (1.29-to 5.60 fold) in response to malathion or deltamethrin exposures, indicating that these esterases are involved in the detoxification process. Silencing of LbEST1, LbEST2 or LbEST3 through dsRNA feeding led to a higher mortality of psocids upon the malathion treatment compared to controls (1.83 to 2.69-fold), demonstrating that these esterase genes play roles in malathion detoxification in L. bostrychophila. Our study provides new evidence for understanding of the function and regulation mechanism of esterases in L. bostrychophila in insecticide detoxification. The current study also suggests that the present RNAi method could be applied for gene functional studies in psocids.

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Section: Discussionsupporting

confidence: 93%

Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

et al. 2020

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“…For example, GSTs can catalyze reduced glutathione to conjugate with insecticides in resistant housefly strains . Similarly, additional evidence was supported by the cytotoxicity assay to verify BdB1 contributed to reducing susceptibility to malathion in Sf9 cells . Based on prokaryotic expression, the recombinant plasmids of BdGSTd1 and BdGSTd10 were transformed into E. coli BL21 (DE3) to express the recombinant enzymes and evaluate the capabilities of malathion detoxification.…”

Section: Discussionmentioning

confidence: 95%

“…45 Similarly, additional evidence was supported by the cytotoxicity assay to verify BdB1 contributed to reducing susceptibility to malathion in Sf9 cells. 46 Based on prokaryotic expression, the recombinant plasmids of BdGSTd1 and BdGSTd10 were transformed into E. coli BL21 (DE3) to express the recombinant enzymes and evaluate the capabilities of malathion detoxification. The main parameters of enzyme kinetics, including V max and K m , showed some differences between BdGSTd1 and BdGSTd10.…”

Section: Discussionmentioning

confidence: 99%

Two delta class glutathione S‐transferases involved in the detoxification of malathion in Bactrocera dorsalis (Hendel)

Meng

Yuan

et al. 2019

Pest Management Science

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BACKGROUND The oriental fruit fly Bactrocera dorsalis (Hendel), a widespread agricultural pest, has evolved resistance to many insecticides, including organophosphorus compounds. Glutathione S‐transferases (GSTs) are involved in xenobiotic detoxification and insecticide resistance in many insects. However, the role of delta class GSTs in detoxifying malathion in B. dorsalis is unknown. Here, we evaluated the roles of two delta class GSTs in malathion detoxification in this species. RESULTS Two delta class GSTs genes, BdGSTd1 and BdGSTd10, were characterized in B. dorsalis. They were highly expressed in 5‐day‐old adults, as well as in midgut and Malpighian tubules. Upon malathion exposure, the two genes were upregulated by 2.63‐ and 2.85‐fold, respectively. Injection of double‐stranded RNA targeting BdGSTd1 or BdGSTd10 significantly reduced their mRNA levels in adults and also significantly increased adult susceptibility to malathion. The expression of these two GSTs in Escherichia coli helped the host to endure malathion stress at a concentration of 10 µg mL−1 according to a Cell Counting Kit‐8 assay. High‐performance liquid chromatography analyses indicated that malathion could be significantly depleted by the two delta GSTs. The role of BdGSTd10 in malathion sequestration was also discussed. CONCLUSION BdGSTd1 and BdGSTd10 play important roles in the detoxification of malathion in B. dorsalis. © 2019 Society of Chemical Industry

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“…The involvement of CarEs in the detoxification of different agrochemicals has been reported in insect species, such as in Myzus persicae (Sulzer), 23 Culex mosquitoes, 24 H. armigera , 25 Lucilia cuprina , 26 Cydia pomonella , 27 Bactrocera dorsalis , 28 and Locusta migratoria 29 . Meanwhile, other studies have implicated CarEs in insects resistance to insecticides 2,17,23 .…”

Section: Introductionmentioning

confidence: 99%

“…21 CarEs are ubiquitous in various organisms including mammals and insects, and play crucial roles in the detoxification of xenobiotic chemicals including various drugs and insecticides. 22 The involvement of CarEs in the detoxification of different agrochemicals has been reported in insect species, such as in Myzus persicae (Sulzer), 23 Culex mosquitoes, 24 H. armigera, 25 Lucilia cuprina, 26 Cydia pomonella, 27 Bactrocera dorsalis, 28 and Locusta migratoria. 29 Meanwhile, other studies have implicated CarEs in insects resistance to insecticides.…”

Section: Introductionmentioning

confidence: 99%

Enhanced hydrolysis of β‐cypermethrin caused by deletions in the glycin‐rich region of carboxylesterase 001G from Helicoverpa armigera

Bai

Zhao

et al. 2021

Pest Management Science

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BACKGROUND Carboxylesterase (CarE) is a major class of enzyme involved in the detoxification of toxic xenobiotics in various insect species. Previous work has shown that the carboxylesterase gene CarE001G found in Helicoverpa armigera is more active and can metabolize synthesized pyrethroids, such as β‐cypermethrin, one of the commonly used commercial insecticides for lepidopteran pest control. In addition, CarE001G is very special as it has a very specific glycine‐rich region located adjacent to its C‐terminal. But whether mutations in this unique sequence can change the biochemistry and function of CarE001G are unknown. RESULTS In this study, four variants of CarE001G with different deletions in the glycine‐rich region were obtained and functionally expressed in Escherichia coli. The recombinant proteins were purified and confirmed by Western blot and mass spectrometry analyses. These mutant enzymes showed high catalytic efficiency toward the model substrate α‐naphthyl acetate. Inhibition study showed that β‐cypermethrin had relatively strong inhibition on CarE activities. In vitro metabolism assay showed that the mutant enzymes significantly enhanced their metabolic activities toward β‐cypermethrin with specific activities between 4.0 and 5.6 nmol L‐1 min−1 mg−1 protein. Molecular docking analyses consistently demonstrated that deletion mutations in the glycine‐rich region may facilitate the anchoring of the β‐cypermethrin molecule in the active binding pocket of the mutant enzymes. CONCLUSION The data show that deletion mutations can cause qualitative change in the capacity of CarEs in the detoxification of β‐cypermethrin. This indicates that deletion mutations in the glycine‐rich region may have the potential to cause synthesized pyrethroid (SP) resistance in H. armigera in the future. © 2020 Society of Chemical Industry

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Functional characterization of BdB1, a well-conserved carboxylesterase among tephritid fruit flies associated with malathion resistance in Bactrocera dorsalis (Hendel)

Cited by 20 publications

References 39 publications

Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

Two delta class glutathione S‐transferases involved in the detoxification of malathion in Bactrocera dorsalis (Hendel)

Enhanced hydrolysis of β‐cypermethrin caused by deletions in the glycin‐rich region of carboxylesterase 001G from Helicoverpa armigera

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