Therapeutic application of human leukocyte antigen-G1 improves atopic dermatitis-like skin lesions in mice

Maeda, Naoyuki; Yamada, Chizumi; Takahashi, Ami; Kuroki, Kazuhiko; Maenaka, Katsumi

doi:10.1016/j.intimp.2017.06.026

Cited by 9 publications

(9 citation statements)

References 33 publications

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“…Application of 2,4-dinitrocholrlbenzene (DNCB) on mouse skin shows symptoms similar to those of human AD, including epidermal hyperplasia, dermal mast cell infiltration, and elevated serum IgE levels [30]. In another model, repeated exposure of NC/Nga mice to Dermatophagoides farinae ( D. farinae ) crude extract (DfE) induced AD-like lesions [31]. In this study, we applied an S. aureus strain isolated from an AD patient onto a skin wound in mice, thus simulating AD patients with a wound on a lesional skin after persistent scratching.…”

Section: Discussionmentioning

confidence: 99%

A Derivative of Butyric Acid, the Fermentation Metabolite of Staphylococcus epidermidis, Inhibits the Growth of a Staphylococcus aureus Strain Isolated from Atopic Dermatitis Patients

Traisaeng

Herr

Kao

et al. 2019

Toxins

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The microbiome is a rich source of metabolites for the development of novel drugs. Butyric acid, for example, is a short-chain fatty acid fermentation metabolite of the skin probiotic bacterium Staphylococcus epidermidis (S. epidermidis). Glycerol fermentation of S. epidermidis resulted in the production of butyric acid and effectively hindered the growth of a Staphylococcus aureus (S. aureus) strain isolated from skin lesions of patients with atopic dermatitis (AD) in vitro and in vivo. This approach, however, is unlikely to be therapeutically useful since butyric acid is malodorous and requires a high concentration in the mM range for growth suppression of AD S. aureus. A derivative of butyric acid, BA–NH–NH–BA, was synthesized by conjugation of two butyric acids to both ends of an –NH–O–NH– linker. BA–NH–NH–BA significantly lowered the concentration of butyric acid required to inhibit the growth of AD S. aureus. Like butyric acid, BA–NH–NH–BA functioned as a histone deacetylase (HDAC) inhibitor by inducing the acetylation of Histone H3 lysine 9 (AcH3K9) in human keratinocytes. Furthermore, BA–NH–NH–BA ameliorated AD S. aureus-induced production of pro-inflammatory interleukin (IL)-6 and remarkably reduced the colonization of AD S. aureus in mouse skin. These results describe a novel derivative of a skin microbiome fermentation metabolite that exhibits anti-inflammatory and S. aureus bactericidal activity.

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Section: Discussionmentioning

confidence: 99%

A Derivative of Butyric Acid, the Fermentation Metabolite of Staphylococcus epidermidis, Inhibits the Growth of a Staphylococcus aureus Strain Isolated from Atopic Dermatitis Patients

Traisaeng

Herr

Kao

et al. 2019

Toxins

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“…Notably, the amounts of HLA-G inversely correlate with the severity of autoimmune diseases [5,6,7]. We also demonstrated that the administration of the HLA-G molecule improved the symptoms of the collagen induced arthritis model mice and the atopic dermatitis model mice [8,9,10]. Furthermore, certain tumor cells also express HLA-G on the surface to suppress immune activation and escape immune surveillance [11,12].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Reactivity and Receptor Competition of HLA-G Isoforms toward Available Antibodies: Implications of Structural Characteristics of HLA-G Isoforms

Furukawa

Meguro

Yamazaki

et al. 2019

IJMS

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The human leucocyte antigen (HLA)-G, which consists of seven splice variants, is a tolerogenic immune checkpoint molecule. It plays an important role in the protection of the fetus from the maternal immune response by binding to inhibitory receptors, including leukocyte Ig-like receptors (LILRs). Recent studies have also revealed that HLA-G is involved in the progression of cancer cells and the protection from autoimmune diseases. In contrast to its well characterized isoform, HLA-G1, the binding activities of other major HLA-G isoforms, such as HLA-G2, toward available anti-HLA-G antibodies are only partially understood. Here, we investigate the binding specificities of anti-HLA-G antibodies by using surface plasmon resonance. MEM-G9 and G233 showed strong affinities to HLA-G1, with a nM range for their dissociation constants, but did not show affinities to HLA-G2. The disulfide-linker HLA-G1 dimer further exhibited significant avidity effects. On the other hand, 4H84 and MEM-G1, which can be used for the Western blotting of HLA-G isoforms, can bind to native HLA-G2, while MEM-G9 and G233 cannot. These results reveal that HLA-G2 has a partially intrinsically disordered structure. Furthermore, MEM-G1, but not 4H84, competes with the LILRB2 binding of HLA-G2. These results provide novel insight into the functional characterization of HLA-G isoforms and their detection systems.

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“…Mostly, DBA/1 mice have been used as a model of autoimmunity [28]. The production of IgE has been shown to be significantly increased in collagen-induced arthritic mice [29]. Serum IgE is considered one of the essential diagnostic parameters in the diagnostic workup of food allergy [30].…”

Section: Discussionmentioning

confidence: 99%

Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice

Du¹,

Xiao²,

Zhou³

et al. 2019

Biol Res

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Background Fava beans (FBs) have long been used as food, and their principal disadvantage is derived from their haemotoxicity. We hypothesized that FB ingestion alters the intestinal gene expression pattern, thereby inducing an immune response. Results In-depth sequence analysis identified 769 differentially expressed genes (DEGs) associated with the intestine in FB-treated DBA/1 mouse intestines. The identified genes were shown to be associated with biological processes (such as response to stimulus and immune system processes), human disease pathways (such as infectious diseases, endocrine and metabolic diseases, and immune diseases), and organismal system pathways (such as the digestive system, endocrine system, environmental adaptation, and immune system). Moreover, plasma total immunoglobulin E (IgE), histamine, interleukin (IL)-4 and IL-13 levels were significantly increased when the mice were treated with FBs. Conclusions These results demonstrated that FBs affect the intestinal immune response and IgE and cytokine secretion in DBA/1 mice. Electronic supplementary material The online version of this article (10.1186/s40659-019-0216-9) contains supplementary material, which is available to authorized users.

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Therapeutic application of human leukocyte antigen-G1 improves atopic dermatitis-like skin lesions in mice

Cited by 9 publications

References 33 publications

A Derivative of Butyric Acid, the Fermentation Metabolite of Staphylococcus epidermidis, Inhibits the Growth of a Staphylococcus aureus Strain Isolated from Atopic Dermatitis Patients

A Derivative of Butyric Acid, the Fermentation Metabolite of Staphylococcus epidermidis, Inhibits the Growth of a Staphylococcus aureus Strain Isolated from Atopic Dermatitis Patients

Evaluation of the Reactivity and Receptor Competition of HLA-G Isoforms toward Available Antibodies: Implications of Structural Characteristics of HLA-G Isoforms

Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice

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