Plasmids of Diverse Inc Groups Disseminate the Fosfomycin Resistance Gene fosA3 among Escherichia coli Isolates from Pigs, Chickens, and Dairy Cows in Northeast China

Abstract

Thirty-nine fosfomycin-resistant Escherichia coli isolates carrying fosA3 were obtained from pigs, chickens, dairy cows, and staff in four northeastern

Antimicrobial Resistance inEscherichia coli

“…Two major fosfomycin resistance mechanisms have been described: (i) mutations in the glpT and uhpA/T genes encoding proteins involved in the fosfomycin uptake system and (ii) the acquisition of fosfomycin-modifying enzymes such as the metalloenzymes FosA, FosB, and FosX or the kinases FomA and FomB (160). Most of the fos-like genes are plasmid-borne, and plasmids carrying the fos genes commonly carry additional resistance genes (124,161,162) that increase the risk of coselection of fosfomycin resistance under the selective pressure by other antimicrobial agents.…”

“…Furthermore, the analysis of 1,693 E. coli isolates from various animal species identified 97 fosA3-positive isolates from beef cattle, pigs, broiler chickens, stray cats, stray dogs, and wild rodents in China (171). Recently, several epidemic fosA3carrying multiresistance plasmids of diverse incompatibility groups have been identified to be disseminated among E. coli from pigs, dairy cattle, and chickens in northeast China (162). Some of these plasmids have been sequenced completely, including the plasmids pECM13 from cattle (113,006 bp, IncI1, and coharboring bla CTX-M-14 , rmtB, aadA2, and bla TEM-1 ), pECB11 from chicken [92,545 bp, F33:A -:B -, and coharboring bla CTX-M-55 , floR, cfr, bla TEM-1 , tet(A), strA, and strB], and pECF12 from chicken [77,822 bp, F33:A -:B -, and coharboring bla CTX-M-3 , rmtB, tet(A), strA, and strB].…”

Antimicrobial Resistance inEscherichia coli

“…Furthermore, the analysis of 1,693 E. coli isolates from various animal species identified 97 fosA3-positive isolates from beef cattle, pigs, broiler chickens, stray cats, stray dogs, and wild rodents in China (171). Recently, several epidemic fosA3carrying multiresistance plasmids of diverse incompatibility groups have been identified to be disseminated among E. coli from pigs, dairy cattle, and chickens in northeast China (162). Some of these plasmids have been sequenced completely, including the plasmids pECM13 from cattle (113,…”

Evolution and Comparative Genomics of F33:A−:B− Plasmids Carrying bla CTX-M-55 or bla CTX-M-65 in Escherichia coli and Klebsiella pneumoniae Isolated from Animals, Food Products, and Humans in China

“…Our previous studies confirmed that F33:A−:B− plasmids were major vehicles for fosA3 - bla CTX-M-65 - rmtB transmission among food animals and pets in Guangdong Province, China ( 5 , 6 ); similar F33:A−:B− plasmids associated with rmtB - bla CTX-M-65 were disseminated in pets in southern China and in a pig farm and its environment in eastern China ( 7 , 8 ). In addition, F33:A−:B− or IncN-F33:A−:B− plasmids were responsible for the dissemination of fosA3 and bla CTX-M-55/-65 genes in E. coli from chickens in China and pigs, chickens, and dairy cows in northeast China ( 9 – 11 ). Furthermore, oqxAB colocated with bla CTX-M-55 on F33:A−:B− and IncN-F33:A−:B− plasmids was identified in food-producing animals, chicken meat, and humans in China ( 12 – 15 ).…”