Eclipta prostrataImproves DSS-Induced Colitis through Regulation of Inflammatory Response in Intestinal Epithelial Cells

Abstract

Eclipta prostrata (EP) and its compounds are known to have several pharmacological effects including anti-inflammatory effects. In the present study, we demonstrated that EP improves the dextran sulfate sodium (DSS)-induced colitis symptoms such as body weight loss, colon length shortening and disease activity index. In DSS-induced colitis tissue, EP controls the protein expressions of cyclooxygenase-2 (COX-2) and hypoxia inducible factor-1[Formula: see text] (HIF-1[Formula: see text]). In addition, the release of prostaglandin E and vascular endothelial growth factor-A were significantly reduced by EP administration. EP also inhibited COX-2 and HIF-1[Formula: see text] expressions in the tumor necrosis factor-[Formula: see text] stimulated HT-29 cells. These inhibitory effects of EP occurred by reducing the phosphorylation of I[Formula: see text]B and the translocation of the nuclear factor-[Formula: see text]B (NF-[Formula: see text]B). Additionally, we found through HPLC analysis that wedelolactone, which is an inhibitor of NF-[Formula: see text]B transcription, was contained in water extract of EP. These results indicate that EP can improve colitis symptoms through the modulation of immune function in intestinal epithelial cells and suggests that EP has the potential therapeutic effect to intestinal inflammation.

Targeting NF-κB pathway for treating ulcerative colitis: comprehensive regulatory characteristics of Chinese medicines

“…Study shows that inhibitory effects of Eclipta Prostrata (EP) (500 mg/kg) on DSS-induced UC occurrence by improving body weight loss, shortened colon length and DAI, and reducing the phosphorylation of IκB and the translocation of the NF-κB. Additionally, a HPLC analysis shows that wedelolactone, contained in water extract of EP, is an inhibitor of NF-κB transcription [115].…”

“…Qi-regulating medicine Norisoboldine DSS-induced colon tissues Inhibit phosphorylation, nuclear translocation and DNA-binding activity of NF-κBp65 [113] Citrus aurantium L. TNBS-induced colon tissues Inhibit the NF-κB pathway [114] RAW264.7 cells Inhibit the protein expressions of NF-κB [114] Tonifying medicine Eclipta Prostrata HT-29 cells Reduce the nuclear translocation of NF-κB [115] Astragalus polysaccharide DSS-induced colonic tissues Reduce NF-κB DNA phosphorylation activity [116] Astragaloside IV CCD-18Co cells Inhibit the phosphorylation of NF-κBp65 [117] Licochalcone A DSS-induced colonic tissues Reverse the increased expression of p65 NF-κB [118] Diammonium glycyrrhizinate AA-induced colonic tissues Suppress the positive percentage and density of NF-κBp65 [119] Vanillic acid DSS-induced colon tissues Suppress the activation of transcription NF-κBp65 [120] Paeoniflorin TNBS-induced colon tissues Inhibit the expressions of p-NF-κB [121] The fruit of Ziziphus jujuba Mill.…”

“…Reduce the degradation of IκB [115] Astragaloside IV CCD-18Co cells Inhibit the phosphorylation of IκB [117] Licochalcone A…”

Ultra‐high‐pressure‐assisted extraction of wedelolactone and isodemethylwedelolactone from Ecliptae Herba and purification by high‐speed counter‐current chromatography

“…In India and Brazil, Ecliptae Herba has been used for the treatment of infective hepatitis (Manvar, Mishra, Kumar, & Pandey, ; Singh, Saxena, Chandan, Agarwal, & Anand, ) and snake venom poisoning (Diogo et al, ; Soares et al, ). Modern pharmacological studies show that Ecliptae Herba has hemostatic, liver‐protective, anti‐inflammatory and anti‐snake venom activities (Kim et al, ; Prabu, Kanchana, & Sadiq, ). Ecliptae Herba contains various constituents, such as triterpenes, flavonoids, polyacetylenes and coumestans (Han et al, ), among which wedelolactone, one of the major coumestans, has been used for the quality assessment of this herbal (Scholar, ).…”

Beneficial Effect of Herbal Formulation KM1608 on Inflammatory Bowl Diseases: A Preliminary Experimental Study

“…Over the past decade, herbal extracts and their active ingredients have been studied in the development of anti-inflammatory agents [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. Aucklandia lappa DC., Terminalia chebula Retz and Zingiber officinale Roscoe are well-known medicinal plants widely used in the traditional medicine of east Asia.…”