An improved <scp>PCR</scp>‐<scp>CTPP</scp> assay for the detection of <scp>ADH</scp>1B Arg48His polymorphism

Jiang, Song; Tong, Yongqing; Zhao, Rui; Xiong, Ge; Qiao, Bin

doi:10.1002/jcla.22268

Cited by 1 publication

(1 citation statement)

References 16 publications

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“…The two fluorescent probes used in TaqMan make genotyping accurate and fast but result in higher labour and material costs [44]. Due to its simplicity and reliability, the PCR-CTPP assay has been a popular technique for assessing SNP [45] and a newly designed PCR-CTPP has been shown to be both rapid and reliable for polymorphism detection in a clinic setting [46]. A previous study indicated that the accuracy [47].…”

Section: Discussionmentioning

confidence: 99%

Association between APE1 ASP148GLU and colorectal cancer risk: A meta-analysis

Lin

Jin²,

Cheng

et al. 2020

CIM

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Background: Colorectal cancer (CRC) is recognized as one of the most common cancer globally. The association between CRC and apurinic endonuclease 1 (APE1) Asp148Glu polymorphism remains unclear; thus, this meta-analysis aimed to explore whether APE1 Asp148Glu polymorphism is related to CRC risk. Methods: Embase, PubMed, Cochrane library, CNKI and Wanfang databases were subject to a systematic search until April, 17, 2020 to evaluate the effect of APE1 Asp148Glu polymorphism on CRC risk. The associated strength was used to evaluate with odds ratios (ORs) with 95% confidence intervals (CIs) between Asp148Glu polymorphism and CRC risk. Subgroup analyses were also performed. Results: In total, 11 articles including 8,136 subjects (3,836 cases and 4,300 controls) were included. Five genetic models were analyzed, including the additive model (G vs. T), the heterozygote comparison (TG vs. TT), the homozygote comparison (GG vs. TT), the dominant model (TG+GG vs. TT), and the recessive model (GG vs. TG+TT). In these models, T refers to thymine and G refers to guanine. The APE1 Asp148Glu polymorphism in heterozygote comparison [OR (95%CI) = 1.36 (1.05, 1.75), P=0.019] and dominant model [OR (95%CI) =1.31 (1.07, 1.61), P=0.010] significantly increased CRC risk. No significant association was seen for the additive model [OR (95%CI) = 1.14 (1.00, 1.31), P=0.057], recessive model [OR (95%CI) = 0.97 (0.71, 1.31), P=0.826] or in homozygote comparison [OR (95%CI) = 1.15 (0.88, 1.52), P=0.309]. Moreover, CRC risk indicated a remarkable association with APE1 Asp148Glu polymorphism in the PCR-RFLP additive model, homozygote comparison and recessive model (PG) may be a potential risk factor for CRC.

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Section: Discussionmentioning

confidence: 99%