Abstract:Nanoparticles composed of Prussian Blue, PB, and the cyanometalate structural analogues, CuFe, FeCoFe, and FeCo, are examined as inorganic clusters that mimic the functions of peroxidases. PB acts as a superior catalyst for the oxidation of dopamine to aminochrome by HO. The oxidation of dopamine by HO in the presence of PB is 6-fold faster than in the presence of CuFe. The cluster FeCo does not catalyze the oxidation of dopamine to aminochrome. The most efficient catalyst for the generation of chemiluminescen… Show more
“…Scanning electron microscopy (SEM) images of PB itself exhibited as nanocubes with a size of~50 nm (Figure 1e), which is consistent with the previous report. [18] After incorporation of Cyt c into the PB nanoparticles, a plate like structure was observed (Figure 1f), indicating that the introduction of protein interfered with the biomineralization.…”
Section: Peroxidase Encapsulated In Peroxidase Mimics Via In Situ Assmentioning
Enzymatic catalysis is of great importance due to its high catalytic activity and high selectivity. However, intrinsic fragile property of enzyme limits its application in a wide range, where inactive inorganic materials have proven promising in enhancing the stability of the enzyme in industry. Here, Prussian blue nanoparticles (PB) as peroxidase mimics were adopted for encapsulation of peroxidase, Cytochrome c (Cyt c). Prussian blue/Cytochrome c (PB@Cyt c) composite developed via in‐situ assembly exhibited a 2.6‐fold higher apparent enzymatic activity than the sum activity of equivalent Cyt c and PB due to synergistic effect. Mechanism investigation showed that interaction between irons ions from PB and carbonyl group and nitrogen from enzyme led to a favorable catalytic conformation of Cyt c. Moreover, PB shielded the encapsulated Cyt c against harsh conditions (e. g. high temperature and organic solvents). This new efficient hybrid catalyst would open opportunities for wide applications in biosensors and biocatalysis.
“…Scanning electron microscopy (SEM) images of PB itself exhibited as nanocubes with a size of~50 nm (Figure 1e), which is consistent with the previous report. [18] After incorporation of Cyt c into the PB nanoparticles, a plate like structure was observed (Figure 1f), indicating that the introduction of protein interfered with the biomineralization.…”
Section: Peroxidase Encapsulated In Peroxidase Mimics Via In Situ Assmentioning
Enzymatic catalysis is of great importance due to its high catalytic activity and high selectivity. However, intrinsic fragile property of enzyme limits its application in a wide range, where inactive inorganic materials have proven promising in enhancing the stability of the enzyme in industry. Here, Prussian blue nanoparticles (PB) as peroxidase mimics were adopted for encapsulation of peroxidase, Cytochrome c (Cyt c). Prussian blue/Cytochrome c (PB@Cyt c) composite developed via in‐situ assembly exhibited a 2.6‐fold higher apparent enzymatic activity than the sum activity of equivalent Cyt c and PB due to synergistic effect. Mechanism investigation showed that interaction between irons ions from PB and carbonyl group and nitrogen from enzyme led to a favorable catalytic conformation of Cyt c. Moreover, PB shielded the encapsulated Cyt c against harsh conditions (e. g. high temperature and organic solvents). This new efficient hybrid catalyst would open opportunities for wide applications in biosensors and biocatalysis.
“…HRP/H 2 O 2 couple is commonly selected as a biomimetic oxidizing agent to examine the early stages of dopamine oxidation. 53 The Ru NPs can catalyze the H 2 O 2 -driven oxidation of DA to aminochrome (AC), in analogy to HRP. The time-dependent absorbance change of AC, generated upon oxidation of dopamine, was shown in Fig.…”
Section: Enzyme-like Activity Of Ru Nps During Oxidation Of Opd and Damentioning
Ru NPs could catalyze the oxidation of 3,3,5,5-tetramethylbenzidine, o-phenylenediamine and dopamine hydrochloride in the presence of H2O2, and also catalyze the oxidization of 3,3,5,5-tetramethylbenzidine and sodium l-ascorbate by dissolved oxygen.
“…In addition to PEDOT:PSS, we tested a PEDOT:PSS composite with Prussian blue (PB) nano/microcrystals, synthesized according to the literature. 20 PB is a well-known catalyst for reduction of peroxide to water, and is one of the to-date best materials as cathodes for peroxide fuel cells. PEDOT:PSS cathodes ( Fig.…”
Efficient single-compartment hydrogen peroxide fuel cells can be fabricated using the conducting polymer poly(3,4-ethylenedioxythiophene), PEDOT as a cathodic electrocatalyst.
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