Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients

Archampong, Timothy; Asmah, Richard Harry; Aidoo, Ebenezer K.; Wiredu, Edwin K.; Gyasi, Richard K.; Adjei, David Nana; Beleza, Sandra; Bayliss, Christopher D.; Krogfelt, Karen A.

doi:10.1186/s13104-017-2542-8

Cited by 21 publications

(21 citation statements)

References 17 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The prevalence of H. pylori in dyspeptic patients in Northwest Ethiopia was 43% in males and 32% in females, 16 and higher in Ghana: 71.1%. 17 In the latter study population, vacAs1 was present in 69.2%, vacAm1 in 44.7%, vacAm2 in 22.0%, and cagA in 74.8%; but vacAs1/cagA+ was found in 72.6% of the H. pylori positive individuals. 17 A potentially declining prevalence was reported among Algerian patients; the recent report indicated a 57% prevalence while in the 1980s, it was >80%.…”

Section: Africamentioning

confidence: 72%

“…17 In the latter study population, vacAs1 was present in 69.2%, vacAm1 in 44.7%, vacAm2 in 22.0%, and cagA in 74.8%; but vacAs1/cagA+ was found in 72.6% of the H. pylori positive individuals. 17 A potentially declining prevalence was reported among Algerian patients; the recent report indicated a 57% prevalence while in the 1980s, it was >80%. Among the 42 bacterial strains isolated from 38 patients, the proportion of haplotypes was: 33 hpEurope and nine hpNEAfrica.…”

Section: Africamentioning

confidence: 72%

“…The prevalence of H. pylori in dyspeptic patients in Northwest Ethiopia was 43% in males and 32% in females, and higher in Ghana: 71.1% . In the latter study population, vac As1 was present in 69.2%, vac Am1 in 44.7%, vac Am2 in 22.0%, and cag A in 74.8%; but vac As1/ cag A+ was found in 72.6% of the H. pylori positive individuals …”

Section: Global Prevalence Of Helicobacter Pylori Infectionmentioning

confidence: 99%

See 2 more Smart Citations

Epidemiology of Helicobacter pylori infection

et al. 2018

View full text Add to dashboard Cite

This review provides the most recent data concerning the epidemiology of Helicobacter pylori infection. Overall, the trend of declining prevalence of H. pylori infection is continuing, with major evidence available from studies in Europe. However, in some parts of the world, for example, in some countries in the Middle East, the prevalence has remained relatively stable. A number of systematic reviews and meta-analyses have been published during the past year indicating the lowest prevalence rates of the infection in Oceania (24.4%), the highest in Africa (79.1%), and the global annual recurrence rate of H. pylori (4.3%). The recurrence rates were found to be directly related to the human development index and prevalence of infection. Several studies have addressed the correlation between H. pylori infection and sociodemographic conditions, source of drinking water and dietary factors. A hypothesis on the role of insects and yeasts in transmitting H. pylori has been suggested and addressed. Helicobacter sp. have been found in flow flies in Brazil. So far there is no evidence available that H. pylori may survive and persist on the outer body of the fly.

show abstract

Section: Africamentioning

confidence: 72%

Section: Africamentioning

confidence: 72%

Section: Global Prevalence Of Helicobacter Pylori Infectionmentioning

confidence: 99%

See 1 more Smart Citation

Epidemiology of Helicobacter pylori infection

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Generally, the disease prevalence across countries in the West African area varies and there is no clear show of a steady overall increment or decrease with age. In Ghana, the reported prevalence rate is 75% [64–66] while in Senegal 62–97% has been reported [67–72]. A prevalence of 92% was seen in Cote D'Ivoire [73], while in Benin, about 56–72% has been reported [74, 75].…”

Section: Resultsmentioning

confidence: 99%

Helicobacter pyloriInfection, Virulence Genes’ Distribution and Accompanying Clinical Outcomes: The West Africa Situation

Ofori

Adinortey

Bockarie

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

Data on Helicobacter pylori (H. pylori) infection and virulence factors in countries across West Africa are scattered. This systematic review seeks to present an update on the status of H. pylori infection focusing on prevalence rate, distribution of virulent genes, and their link to clinical outcomes across countries in the western part of Africa. This information is expected to broaden the knowledge base of clinicians and researchers regarding H. pylori infection and associated virulence factors in West African countries. Search Method. A comprehensive search of the scientific literature in PubMed and ScienceDirect was conducted using the search terms including “Helicobacter pylori infection in West Africa”. Databases were sourced from January 1988 to December 2018. Results. Data on the incidence of H. pylori infection and related pathological factors were found for some countries, whereas others had no information on it. Smoking, alcohol, exposure to high levels of carcinogens and diet were reported to be involved in the pathogenesis of gastroduodenal diseases and gastric cancer. Besides the environmental factors and genetic characteristics, there are important characteristics of H. pylori such as the ability to infect, replicate, and persist in a host that have been associated with the pathogenesis of various gastroduodenal diseases. Concluding Remarks. This systematic search has provided information so far available on H. pylori virulence factors and clinical outcomes in West Africa. Accordingly, this piece has identified gaps in the body of knowledge highlighting the need for more studies to clarify the role of H. pylori virulence factors and associated clinical outcomes in the burden of this bacterial infection in West Africa, as data from these countries do not give the needed direct relation.

show abstract

“…In addition, it is also capable to detect clarithromycin resistance genotype due to point mutations in the H. pylori 23S rRNA gene [28]. Eight (15.38%) out of 52 biopsy specimens detected 23S rRNA which was much lower than that reported by Archampong et al in Ghana (48.4%) with cagA gene [29], Ruparelia et al in Brazil (50%) with ureA + ureC gene [30], and Sugimoto et al (44%) with 16S rRNA [31]. Hundred percent diagnostic accuracy cannot be achieved by the application of single PCR assay [31] since the genomic flexibility between strains of H. pylori complicates the choice of target genes [20].…”

Section: Discussionmentioning

confidence: 99%

Application of PCR and Microscopy to Detect Helicobacter pylori in Gastric Biopsy Specimen among Acid Peptic Disorders at Tertiary Care Centre in Eastern Nepal

Pokhrel

Khanal

Rai

et al. 2019

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

Background. Helicobacter pylori infection is most prevalent in developing countries. It is an etiological agent of peptic ulcer, gastric adenocarcinoma, and mucosal-associated lymphoid tissue (MALT) lymphoma. Despite the development of different assays to confirm H. pylori infection, the diagnosis of infection is challenged by precision of the applied assay. Hence, the aim of this study was to understand the diagnostic accuracy of PCR and microscopy to detect the H. pylori in the gastric antrum biopsy specimen from gastric disorder patients. Methods. A total of 52 patients with gastric disorders underwent upper gastrointestinal endoscopy with biopsy. The H. pylori infection in gastric biopsies was identified after examination by microscopy and 23S rRNA specific PCR. The agreement between two test results were analysed by McNemar’s test and Kappa coefficient. Result. H. pylori infection was confirmed in 9 (17.30%) patients by both assays, 6.25% in antral gastritis, 22.22% in gastric ulcer, 100% in gastric ulcer with duodenitis, 50% in gastric ulcer with duodenal ulcer, and 33.33% in severe erosive duodenitis with antral gastritis. Out of nine H. pylori infection confirmed patients, 3 patients were confirmed by microscopy and 8 patients by PCR. In case of two patients, both microscopy and PCR assay confirmed the H. pylori infection. The agreement between two test results was 86.54% and disagreed by 13.46% (p value > 0.05). Conclusion. We found that PCR assay to detect H. pylori is more sensitive than microscopy. However, we advocate for the combination of both assays to increase the strength of diagnostic accuracy due to the absence of the gold standard assay for H. pylori infection.

show abstract

Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients

Cited by 21 publications

References 17 publications

Epidemiology of Helicobacter pylori infection

Epidemiology of Helicobacter pylori infection

Helicobacter pyloriInfection, Virulence Genes’ Distribution and Accompanying Clinical Outcomes: The West Africa Situation

Application of PCR and Microscopy to Detect Helicobacter pylori in Gastric Biopsy Specimen among Acid Peptic Disorders at Tertiary Care Centre in Eastern Nepal

Contact Info

Product

Resources

About