<i>Piwi1</i>is essential for gametogenesis in mollusk<i>Chlamys farreri</i>

Ma, Xing-Hong; Ji, Aichang; Zhang, Zhifeng; Yang, Dandan; Liang, Suzhe; Wang, Yuhan; Qin, Zhenkui

doi:10.7717/peerj.3412

Cited by 13 publications

(5 citation statements)

References 31 publications

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“…Despite the likely more than seventy thousand living molluskan species 39 there exist only a few functional descriptions of PIWI proteins or piRNAs for this taxon based on experiments in the sea slug Aplysia californica 38 , the Farrer’s scallop Chlamys farreri 40 and in the dog whelk Nucella lapillus 41 . Importantly, Waldron et al 41 recently showed that piRNA-like small RNAs matching virus and transposon sequences are somatically expressed in Nucella lapillus .…”

Section: Introductionmentioning

confidence: 99%

PIWI genes and piRNAs are ubiquitously expressed in mollusks and show patterns of lineage-specific adaptation

et al. 2018

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PIWI proteins and PIWI-interacting RNAs (piRNAs) suppress transposon activity in animals, thus protecting their genomes from detrimental insertion mutagenesis. Here, we reveal that PIWI genes and piRNAs are ubiquitously expressed in mollusks, similar to the situation in arthropods. We describe lineage-specific adaptations of transposon composition in piRNA clusters in the great pond snail and the pacific oyster, likely reflecting differential transposon activity in gastropods and bivalves. We further show that different piRNA clusters with unique transposon composition are dynamically expressed during oyster development. Finally, bioinformatics analyses suggest that different populations of piRNAs presumably bound to different PIWI paralogs participate in homotypic and heterotypic ping-pong amplification loops in a tissue- and sex-specific manner. Together with recent findings from other animal species, our results support the idea that somatic piRNA expression represents the ancestral state in metazoans.

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Section: Introductionmentioning

confidence: 99%

PIWI genes and piRNAs are ubiquitously expressed in mollusks and show patterns of lineage-specific adaptation

et al. 2018

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“…Different stages of sex cells were distinguished in the same acini of male gonads of L. grandis (spermatogonia, spermatocytes, spermatids and spermatozoa), as reported by Ma et al (2017) for the scallop Chlamys farreri (Johnes & Preston, 1904) and by Chatchavalvanich et al (2006) for the freshwater mussel Hyriopsis bialatus Simpson, 1900. This asynchronous gametogenesis in males of L. grandis occurs because different cohorts of gametes that are forming occur simultaneously, and this indicates that spermatozoa production is continuous during the development and maturity of the gonadal phases in males.…”

Section: 15mentioning

confidence: 64%

Gonadal development and sexuality of Larkinia grandis (Arcida: Arcidae) inhabiting southeastern Gulf of California

Sotelo-Gonzalez¹,

García-Ulloa²,

Góngora-Gómez³

et al. 2021

Zoologia

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Larkinia grandis (Broderip & G.B. Sowerby I, 1829), an important fishing resource for Mexican communities, is an Arcidae clam. It is also considered a species with aquaculture potential. In this work we investigated the gonadal phases and sexuality in a population of L. grandis in the Gulf of California. Our findings support the hypothesis that there is one male per female in the population studied. It also documents that the shape, position and color of the gonads of L. grandis are consistent with observations in other Arcidae species. Additionally, five gonadal phases are differentiated and described in males and females (development, mature, spawning, post-spawning and resting), with a noticeable presence of brown cells during post-spawning and the onset of the resting phase, suggesting that those cells are involved in the reabsorption of remnants. Additionally, asynchronous gametogenesis in males, synchronic gametogenesis in females and batch spawning are defined. The results of this contribution can be used in the efforts to protect this bivalve.

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“…Mollusks are one of the largest groups of marine animals, of which Pinctada fucata is well studied due to its economic potential for pearl production and a model organism to investigate the fascinating biology of mollusks. Recent studies have revealed that PIWI proteins are ubiquitously expressed in mollusks (Rajasethupathy et al, 2012;Ma et al, 2017;Jehn et al, 2018). Moreover, abundant piRNA-sized small RNAs were observed in the oysters Crassostrea gigas (Xu et al, 2014;Zhou et al, 2014), Pinctada martensii (Jiao et al, 2014), and the scallop Chlamys farreri (Chen et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Conserved and Widespread Expression of piRNA-Like Molecules and PIWI-Like Genes Reveal Dual Functions of Transposon Silencing and Gene Regulation in Pinctada fucata (Mollusca)

et al. 2021

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PIWI proteins and PIWI-interacting RNAs (piRNAs) suppress transposon activity in animals, thus safeguarding the genome from detrimental insertion mutagenesis. Recent studies revealed additional targets and functions of piRNAs in various animals. piRNAs are ubiquitously expressed in somatic tissues of the pearl oyster Pinctada fucata, however, the role of somatic piRNAs has not well characterized. This study reports the PIWI/piRNA pathway, including piRNA biogenesis and piRNA-mediated transposon silencing, and gene regulation in P. fucata. The biogenesis factors of PIWI, Zucchini, and HEN1, which are ubiquitous in somatic and gonadal tissues, were first identified in P. fucata using transcriptome analysis. Bioinformatics analyses suggested that different populations of piRNAs participate in the ping-pong amplification loop in a tissue-specific manner. In addition, a total of 69 piRNA clusters were identified in the genome of P. fucata based on the expression of piRNAs, which contained 26% transposons and enhanced for DNA/Crypton, LINE/CR1, SINE/Deu, and DNA/Academ. The expression patterns of the piRNAs and piRNA clusters in somatic tissues were not substantially different, but varied significantly between the somatic and gonadal tissues. Furthermore, locked-nucleic-acid modified oligonucleotide (LNA-antagonist) was used to silence single piRNA (piRNA0001) expression in P. fucata. Hundreds of endogenous genes were differentially expressed after piRNA silencing in P. fucata. Target prediction showed that some endogenous genes were targeted by piRNA0001, including twelve upregulated and nine downregulated genes after piRNA0001 silencing. The results indicated that piRNAs from somatic tissues may be related to gene regulation, whereas piRNAs from gonadal tissues are more closely associated to transposon silencing. This study will enhance our understanding of the role of piRNAs in mollusks, transposon silencing, and the regulatory function of the PIWI/piRNA pathway on protein-coding genes outside of germ line cells in P. fucata.

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Piwi1is essential for gametogenesis in molluskChlamys farreri

Cited by 13 publications

References 31 publications

PIWI genes and piRNAs are ubiquitously expressed in mollusks and show patterns of lineage-specific adaptation

PIWI genes and piRNAs are ubiquitously expressed in mollusks and show patterns of lineage-specific adaptation

Gonadal development and sexuality of Larkinia grandis (Arcida: Arcidae) inhabiting southeastern Gulf of California

Conserved and Widespread Expression of piRNA-Like Molecules and PIWI-Like Genes Reveal Dual Functions of Transposon Silencing and Gene Regulation in Pinctada fucata (Mollusca)

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