Biotechnological Potential of Cold Adapted Pseudoalteromonas spp. Isolated from ‘Deep Sea’ Sponges

Abstract

The marine genus Pseudoalteromonas is known for its versatile biotechnological potential with respect to the production of antimicrobials and enzymes of industrial interest. We have sequenced the genomes of three Pseudoalteromonas sp. strains isolated from different deep sea sponges on the Illumina MiSeq platform. The isolates have been screened for various industrially important enzymes and comparative genomics has been applied to investigate potential relationships between the isolates and their host organisms, while comparing them to free-living Pseudoalteromonas spp. from shallow and deep sea environments. The genomes of the sponge associated Pseudoalteromonas strains contained much lower levels of potential eukaryotic-like proteins which are known to be enriched in symbiotic sponge associated microorganisms, than might be expected for true sponge symbionts. While all the Pseudoalteromonas shared a large distinct subset of genes, nonetheless the number of unique and accessory genes is quite large and defines the pan-genome as open. Enzymatic screens indicate that a vast array of enzyme activities is expressed by the isolates, including β-galactosidase, β-glucosidase, and protease activities. A β-glucosidase gene from one of the Pseudoalteromonas isolates, strain EB27 was heterologously expressed in Escherichia coli and, following biochemical characterization, the recombinant enzyme was found to be cold-adapted, thermolabile, halotolerant, and alkaline active.

Biochemical and Taxonomic Characterization of Novel Haloarchaeal Strains and Purification of the Recombinant Halotolerant α-Amylase Discovered in the Isolate

“…For the genus Halogeometricum , the pangenome and core genome contain 7197 and 1874 genes, respectively ( Figure 2D ). Similar findings were reported for the pangenome based analysis in other studies as well ( Capes et al, 2012 ; Becker et al, 2014 ; Borchert et al, 2017 ). The pangenome analysis revealed that wsp1, wsp3, and wsp4 contain 246, 530, and 106 unique genes in their genomes.…”

Cultivable microbiota associated with Aurelia aurita and Mnemiopsis leidyi

“…These occur in open waters, but are also able to colonize animal tissues (Galkiewicz, Pratte, Gray, & Kellogg, 2011; Tarnecki, Patterson, & Arias, 2016). Both genera are well‐known marine inhabitants found in symbiotic associations with sponges, corals, and algae and additionally are known for their versatile biotechnological potential with respect to the production of antimicrobials and enzymes of industrial interest (Borchert et al, 2017; Holmström, James, Neilan, White, & Kjelleberg, 1998; Röthig et al, 2016).…”

Purification, characterization, and gene cloning of a new cold-adapted β-galactosidase from Erwinia sp. E602 isolated in northeast China

“…For example, such enzymes can catalyze reactions at lower temperatures, which might avoid loss of nutrition in foodstuffs and eliminate microbial spoilages of foods to a great extent (Ramli et al, 2011). Furthermore, the cold-adapted enzymes have relative high stability and catalytic efficiency at lower temperatures, reducing the energy cost of the processing and unexpected side effects as well as the property of rapid inactivation at moderate temperatures (Borchert et al, 2017). The number of studies on cold-adapted β-galactosidases continues to increase.…”