Wogonin suppresses stem cell-like traits of CD133 positive osteosarcoma cell via inhibiting matrix metallopeptidase-9 expression

Huynh, Do Luong; Kwon, Taeho; Zhang, Jiao Jiao; Sharma, Neelesh; Gera, Meeta; Ghosh, Mrinmoy; Kim, Nameun; Cho, Somi Kim; Lee, Dong Sun; Park, Yang Ho

doi:10.1186/s12906-017-1788-y

Cited by 27 publications

(20 citation statements)

References 29 publications

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“…Wogonin is known as compound derived from Scutellaria baicalensis , which have shown its anti-cancer effect (i.e., inhibition of angiogenesis, induction of apoptosis, inhibition of cancer growth etc.,) by modulating different signaling pathways such as PKB and AMPK pathways, and prevention of telomerase function, as well as p53-dependent/independent apoptosis [ 189 , 190 ].…”

Section: Identifying Os Stem Cell Populations As Therapeutic Targetsmentioning

confidence: 99%

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Novel molecular insights and new therapeutic strategies in osteosarcoma

et al. 2018

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Osteosarcoma (OS) is one of the most prevalent malignant cancers with lower survival and poor overall prognosis mainly in children and adolescents. Identifying the molecular mechanisms and OS stem cells (OSCs) as new concepts involved in disease pathogenesis and progression may potentially lead to new therapeutic targets. Therefore, therapeutic targeting of OSCs can be one of the most important and effective strategies for the treatment of OS. This review describes the new molecular targets of OS as well as novel therapeutic approaches in the design of future investigations and treatment.

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Section: Identifying Os Stem Cell Populations As Therapeutic Targetsmentioning

confidence: 99%

“…Wogonin has been suggested to effective bioactive compound in preventing OS-CSCs in the bloodstream CSC OS metastases. Appling wogonin as a treatment approach or as a combination with other agents can be a good therapeutic approach, although more research is required in this regard [ 190 ].…”

Section: Identifying Os Stem Cell Populations As Therapeutic Targetsmentioning

confidence: 99%

Novel molecular insights and new therapeutic strategies in osteosarcoma

et al. 2018

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“…Metalloproteinases (MMP) play a key role in the reponse of cells to microenvironment which in turn in uence cell migration and survival. Several metalloproteinases like MMP16 and MMP9 have been reported to be regulated by miR-328-3p and Wogonin respecitively, which can suppress osteosarcoma malignant progression [27,28].…”

Section: Discussionmentioning

confidence: 99%

Identification of TIMP2 and RAX as key regulators in chemosensitive osteosarcoma

Wang

Liu

Zeng

2020

Preprint

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Background This study is trying to investigate the regulation mechanism of chemosensitive osteosarcoma. mRNA and miRNA profiles were explored between chemosensitive and chemoresistant osteosarcoma patients based on GSE39058 dataset. IPA was used for pathway and function enrichment. Another dataset GSE21257 with clinical characteristics was used for survival analysis, and 68 new enrolled osteosarcoma patients with chemotherapy responses were used for RT-PCR validation. Finally, lentiviruses infected U-2 OS cells were subjected to cell counting and real-time cell analyzer.Results A total of 567 differentially expressed genes and 34 differentially expressed miRNAs were screened out. These genes mainly involved in the biology functions of cell survival and cell proliferation, and enriched in the pathways of VEGF signaling, Paxillin signaling, and Inhibition of matrix matalloproteases. In 53 validation osteosarcoma patients from GSE21257, high-expression of TIMP2 and BAX, two common genes among the enriched biology functions, have favorable prognosis with p-values 0.052 and 0.027 respectively. In the 68 new enrolled patients, both TIMP2 and BAX were RT-PCR validated to be high-expressed in chemosensitive group with p-values 0.04 and 0.003 respectively. In addition, cell count and cell index either in TIMP2 or BAX infected U-2 OS cells were smaller compared with control cells within four consecutive days.Conclusions In summary, the biology functions of cell survival and cell proliferation were identified to be mainly inhibited in chemosensitive osteosarcoma patients. Two up-regulated genes, TIMP2 and BAX, were validated to be important regulators of cell proliferation and cell apoptosis in chemosensitive group. Further experimental evidences are still needed to consolidate this results.

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“…Recently, there is an increasing focus on naturally occurring plant products that may reduce side effects and provide a preventive strategy for the treatment of OS. Wogonin, an extract from the root of Scutellaria baicalensis , exhibits the abilities to prevent the invasion and metastasis of CD133+ Cal72 human OS stem cell by the downregulation of MMP‐9 . Celastrol is a triterpene extracted from the Chinese “Thunder God Vine,” could suppress metastasis and induce apoptosis of human OS cells by reducing the expression levels of MMP‐2 and ‐9 via downregulation of the PI3K/Akt/NF‐κB signaling pathway in vitro .…”

Section: Targeting Mmps: Prospects In Clinical Applicationmentioning

confidence: 99%

“…Matrix metalloproteinases (MMPs) are proteolytic enzymes, which play a major role in extracellular matrix (ECM) remodeling. MMPs have also been shown to be involved in the regulation of multiple stages of cancer progression including cell growth, differentiation, apoptosis, migration, invasion, angiogenesis and metastasis . Elevated MMPs levels have been shown to be associated with metastasis and poor prognosis in several types of cancer including OS .…”

Section: Introductionmentioning

confidence: 99%

Pathological and therapeutic aspects of matrix metalloproteinases: Implications in osteosarcoma

Tang¹,

Tang²,

Jiang³

et al. 2019

Asia-Pac J Clncl Oncology

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Osteosarcoma (OS) is one of the most common malignant bone tumors in children and adolescents, and the eighth leading form of childhood cancer. Matrix metalloproteinases (MMPs) are proteolytic enzymes implicated in certain cancers including OS. In this review, we discuss the mechanism of actions of MMPs in progression of OS, and the therapeutic use of MMPs inhibitors in the treatment of OS with subsequent clinical studies and future management. The expression of MMPs is upregulated in cancer cells by a variety of cytokines and growth factors, and upregulation of MMPs induces degradation of the extracellular matrix that contributes to cell proliferation by releasing growth factors. MMPs promote the detachment and migration of endothelial cells, cross the basement membrane as well as invade the surrounding lymphatic vessels and causes cancer metastasis. The use of selective MMP inhibitors with limited side effects might be promising therapeutic strategy in the treatment of OS. More clinical trials are necessary to evaluate the role of selective MMPs inhibitors in the prevention and treatment of OS along with their assessment of toxicity.

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Wogonin suppresses stem cell-like traits of CD133 positive osteosarcoma cell via inhibiting matrix metallopeptidase-9 expression

Cited by 27 publications

References 29 publications

Novel molecular insights and new therapeutic strategies in osteosarcoma

Novel molecular insights and new therapeutic strategies in osteosarcoma

Identification of TIMP2 and RAX as key regulators in chemosensitive osteosarcoma

Pathological and therapeutic aspects of matrix metalloproteinases: Implications in osteosarcoma

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