Influence of Surface Charge of the Nanostructures on the Biocatalytic Activity

Abstract

The physicochemical properties of abiotic nanostructures determine the structure and function of biological counterparts in biotic-abiotic nanohybrids. A comprehensive understanding of the interfacial interactions and the predictive capability of their structure and function is paramount for virtually all fields of bionanotechnology. In this study, using plasmonic nanostructures as a model abiotic system, we investigate the effect of the surface charge of nanostructures on the biocatalytic reaction kinetics of a bound enzyme. We found that the surface charge of nanostructures profoundly influences the structure, orientation, and activity of the bound enzyme. Furthermore, the interactions of the enzyme with nanoparticles result in stable conjugates that retain their functionality at elevated temperatures, unlike their free counterparts that lose their secondary structure and biocatalytic activity.

Vascular Endothelial Growth Factor Incorporated Multilayer Film Induces Preangiogenesis in Endothelial Cells

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“…15 In addition, specific protein binding sites may be disrupted or blocked during synthesis. 16 To design a new growth factor delivery system, the layer-bylayer (LbL) self-assembly method, which builds a multilayer film from the molecular level, has been the primary approach. 17−19 In this method, various "building blocks" that exhibit complementary interactions, such as electrostatic interactions, hydrogen bonding, or biological recognition at the molecular level, are repeatedly deposited on the desired surface.…”

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“…Considering that the structure of horseradish peroxidase (HRP) is generic to the class of peroxidases and metalloenzymes, we have employed HRP as a model heme‐containing enzyme for probing the biocatalytic activity . Our previous findings indicate that the adsorption of HRP on positively charged nanoparticles resulted in a higher biocatalytic activity when compared to HRP adsorbed on negatively charged nanoparticles due to the accessibility of the active site to the substrate molecules . Based on these findings, we employed positively charged nanostructures in this study.…”