Enzyme-less electrochemical displacement heterogeneous immunosensor for diclofenac detection

Nguyen, Thom Thi; Thu, Vũ Thị; Anquetin, Guillaume; Tran, Hoang Vinh; Reisberg, S.; Noël, Vincent; Mattana, Giorgio; Nguyen, Quang Trong; Лам, Тран Дай; Pham, Minh‐Chau; Piro, Benoı̂t

doi:10.1016/j.bios.2017.06.010

Cited by 28 publications

(6 citation statements)

References 22 publications

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“…This is most likely a consequence of high matrix interfering effects of saline matrixes in the detection scheme that can be overcome by using the flow-based kinetic-controlled detection system herein reported. It should also be stressed the fact that the reproducibility of manual immunoassays/biosensors might amount to RSD values as high as 23–100%. ,,, Most importantly, the minimum spiked concentration level of DCF in all of the previous works spanned from 15 to 1000 ng/L, ,− ,− again indicating that none of the immunoassays is suitable for detection of DCF at the environmental quality standard level set in seawater, that is, 10 ng/L.…”

Section: Resultsmentioning

confidence: 98%

“…It should also be stressed the fact that the reproducibility of manual immunoassays/ biosensors might amount to RSD values as high as 23− 100%. 6,49,50,54 Most importantly, the minimum spiked concentration level of DCF in all of the previous works spanned from 15 to 1000 ng/L, 6,49−52,54−56 again indicating that none of the immunoassays is suitable for detection of DCF at the environmental quality standard level set in seawater, that is, 10 ng/L. The precision of the proposed fluidic method was studied at three distinct concentration levels of DCF in seawater that covered 3 orders of magnitude, viz., 0.01, 0.1, and 1.0 ng mL −1 .…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Reliable Sensing Platform for Plasmonic Enzyme-Linked Immunosorbent Assays Based on Automatic Flow-Based Methodology

et al. 2019

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Plasmonic enzyme-linked immunosorbent assays (ELISA) using the localized surface plasmon resonance (LSPR) of metal nanoparticles has emerged as an appealing alternative to conventional ELISA counterparts for ultrasensitive naked-eye detection of biomolecules and small contaminants. However, batchwise plasmonic ELISA involving end-point detection lacks ruggedness inasmuch as the generation or etching of NP is greatly dependent on every experimental parameter of the analytical workflow. To tackle the above shortcomings, this paper reports on an automatic flow methodology as a reliable detection scheme of hydrogen peroxide related enzymatic bioassays for ultrasensitive detection of small molecules. Here, a competitive ELISA is combined with the in-line generation of plasmonic gold nanoparticles (AuNPs) followed by the real-time monitoring of the NP nucleation and growth rates and size distribution using a USB miniaturized photometer. Glucose oxidase was labeled to the secondary antibody and yielded hydrogen peroxide that acted as the measurand and the reducing agent of the Au(III)/citrate system in the flow network. High-throughput plasmonic assays were feasible by assembling a hybrid flow system composed of two microsyringe pumps, a perfluoroalkoxy alkane reaction coil, and a 26-port multiposition valve and operated under computer-controllable flow conditions. The ultratrace determination of diclofenac in high matrix samples, e.g., seawater, without any prior sample treatment was selected as a proof-of-concept application of the flow-based platform for determination of emerging contaminants via plasmonic ELISA. The detection limit (0.001 μg L–1) was 1 order of magnitude lower than that endorsed by the first EU Watch List for diclofenac as a potentially emerging contaminant in seawater and also than that of a conventional colorimetric ELISA, which in turn is inappropriate for determination of diclofenac in seawater at the levels endorsed by the EU regulation. The proposed automatic fluidic approach is characterized by the reproducible timing in AuNPs nucleation and growth along with the unsupervised LSPR absorbance detection of AuNPs with a dynamic range for diclofenac spanning from 0.01 to 10 μg L–1. Repeatability and intermediate precision (given as normalized signal readouts) in seawater were <4% and <14%, respectively, as compared to RSDs as high as 30% as obtained with the batchwise plasmonic ELISA counterpart.

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Section: Resultsmentioning

confidence: 98%

Section: ■ Results and Discussionmentioning

confidence: 99%

Reliable Sensing Platform for Plasmonic Enzyme-Linked Immunosorbent Assays Based on Automatic Flow-Based Methodology

et al. 2019

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“…Although these methods are selective and sensitive, they are not easily amenable to on-site detection. In this context, various biosensing set ups have been implemented using antibodies, 18 aptamers 19,20 or even whole cells [21][22][23] as bioreceptors coupled to electrochemical [24][25][26] or optical 27,28 signal transduction. With such set ups, DCF could be quantified in various matrices with variable sensitivities.…”

Section: -17mentioning

confidence: 99%

“…Chromatographic methods are routinely used to quantify DCF residues in surface and ground waters. , Alternatively, label-based immunochemical assays allow to quantify DCF in water samples with sensitivities from the μg/L down to the sub-ng/L range, depending on the detection method. − Although these methods are selective and sensitive, they are not easily amenable to on-site detection. In this context, various biosensing setups have been implemented using antibodies, aptamers, , or even whole cells − as bioreceptors coupled to electrochemical − or optical , signal transduction. With such setups, DCF could be quantified in various matrices with variable sensitivities.…”

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confidence: 99%

Design and Analytical Performances of a Diclofenac Biosensor for Water Resources Monitoring

et al. 2021

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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“…Faster immunoassays developed for DCF comprise an upconversion-linked immunosorbent assay (ULISA) [26], a fluorescence polarization immunoassay (FPIA) [27], and a suspension array fluorescence immunoassay (SAFIA) [28]. Steps forward to mobile and automated sensing of diclofenac have been made with several immunosensors for the detection of DCF reported in recent years [29][30][31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

A rapid magnetic bead-based immunoassay for sensitive determination of diclofenac

et al. 2021

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Increasing contamination of environmental waters with pharmaceuticals represents an emerging threat for the drinking water quality and safety. In this regard, fast and reliable analytical methods are required to allow quick countermeasures in case of contamination. Here, we report the development of a magnetic bead-based immunoassay (MBBA) for the fast and cost-effective determination of the analgesic diclofenac (DCF) in water samples, based on diclofenac-coupled magnetic beads and a robust monoclonal anti-DCF antibody. A novel synthetic strategy for preparation of the beads resulted in an assay that enabled for the determination of diclofenac with a significantly lower limit of detection (400 ng/L) than the respective enzyme-linked immunosorbent assay (ELISA). With shorter incubation times and only one manual washing step required, the assay demands for remarkably shorter time to result (< 45 min) and less equipment than ELISA. Evaluation of assay precision and accuracy with a series of spiked water samples yielded results with low to moderate intra- and inter-assay variations and in good agreement with LC–MS/MS reference analysis. The assay principle can be transferred to other, e.g., microfluidic, formats, as well as applied to other analytes and may replace ELISA as the standard immunochemical method. Graphical abstract

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Enzyme-less electrochemical displacement heterogeneous immunosensor for diclofenac detection

Cited by 28 publications

References 22 publications

Reliable Sensing Platform for Plasmonic Enzyme-Linked Immunosorbent Assays Based on Automatic Flow-Based Methodology

Reliable Sensing Platform for Plasmonic Enzyme-Linked Immunosorbent Assays Based on Automatic Flow-Based Methodology

Design and Analytical Performances of a Diclofenac Biosensor for Water Resources Monitoring

A rapid magnetic bead-based immunoassay for sensitive determination of diclofenac

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