Action of nitromezuril against Eimeria tenella with clinically anticoccidial indices and histopathology

She, Rufeng; Fei, Chenzhong; Chen, Huiya; Wang, Xiaoyang; Wang, Mi; Zhang, Keyu; Zhang, Lifang; Wang, Chunmei; Liu, Yingchun; Zheng, Wenli; Xue, Feiqun

doi:10.1007/s00436-017-5520-0

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…infections (Debbou-Iouknane et al 2018). This technique is also applied to assess the effectiveness of anticoccidials drugs (Zhang et al 2012, She et al 2017, Fortuoso et al 2019 and vaccines (Jeffers 1975, Song et al 2016, Suprihati & Yunus 2018. However, our research data shows that histopathology also has practical applicability in the diagnosis of coccidiosis.…”

Section: Discussionmentioning

confidence: 99%

Comparison of macroscopy, histopathology and PCR for diagnosing Eimeria spp. in broiler chickens

Balestrin

Santiani

et al. 2022

Pesq. Vet. Bras.

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Coccidiosis is a disease of great importance in industrial poultry. The correct diagnosis directs the poultry industry to its best treatment and control. Thus, a survey of Eimeria spp. was carried out in intestines of 64 broiler flocks, with an average age of 29 days. Eight broilers from each flock were randomly removed from the slaughter line, in a total of 512 samples. Macroscopic and histopathological lesions in the intestine were classified into Scores 0 to 4. Polymerase chain reaction (PCR) was used to research the oocysts from the seven species of Eimeria spp. in the intestinal content. The macroscopic evaluations showed that 59.4% (38/64) of the flocks were positive for E. acervulina, 32.8% (21/64) for E. maxima, 29.7% (19/64) for E. tenella, and 34.4% (22/64) for E. brunetti. The histopathological evaluation showed that 87.5% (56/64) of the flocks had at least one broiler with parasitic structures compatible with Eimeria spp. in the duodenum, 70.3% (45/64) in the jejunum, 18.8% (12/64) in the ileum, 46.9% (30/64) in the cecum, and 4.7% (3/64) in the colon. In PCR, 21.9% (14/64) of the flocks were positive for E. acervulina, 12.5% (8/64) for E. maxima, 3.1% (2/64) for E. mitis, and 32.8% (21/64) for E. tenella. The Kappa Cohen test between macroscopy, histopathology, and PCR demonstrated concordance ranging from weak to moderate with the exception of histopathology and PCR of the cecum, which was strong. In the comparison between macroscopy and histopathology, there were significative differences between Scores 0 and 1 (apart from the cecum). For Score 3, there were significative differences in duodenum, jejunum and cecum (p<0.05). In conclusion, the macroscopic diagnosis and PCR can generate false-negative results, and the histopathological exam proved to be effective, making it essential to associate different techniques for the correct diagnosis of Eimeria spp. in broiler chickens.

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Section: Discussionmentioning

confidence: 99%

Comparison of macroscopy, histopathology and PCR for diagnosing Eimeria spp. in broiler chickens

Balestrin

Santiani

et al. 2022

Pesq. Vet. Bras.

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“…The uninfected group received the same amount of normal saline. On the seventh day PI, the cecum tissues of the three chickens with the highest cecal lesion scores [14] in the infected group (denoted JS1-JS3) and the cecum tissues of three chickens randomly selected from the uninfected group (denoted JC1-JC3) were collected for total RNA extraction. All animal protocols were approved by the Animal Welfare Committee of Yangzhou University, and all efforts were made to minimize the suffering of the chickens.…”

Section: Methodsmentioning

confidence: 99%

RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo

Wang

Zou

et al. 2019

Genes

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Eimeria tenella (E. tenella) is one of the most frequent and pathogenic species of protozoan parasites of the genus Eimeria that exclusively occupies the cecum, exerting a high economic impact on the poultry industry. To investigate differentially expressed genes (DEGs) in the cecal tissue of Jinghai yellow chickens infected with E. tenella, the molecular response process, and the immune response mechanism during coccidial infection, RNA-seq was used to analyze the cecal tissues of an E. tenella infection group (JS) and an uninfected group (JC) on the seventh day post-infection. The DEGs were screened by functional and pathway enrichment analyses. The results indicated that there were 5477 DEGs (p-value < 0.05) between the JS and the JC groups, of which 2942 were upregulated, and 2535 were downregulated. GO analysis indicated that the top 30 significantly enriched GO terms mainly involved signal transduction, angiogenesis, inflammatory response, and blood vessel development. KEGG analysis revealed that the top significantly enriched signaling pathways included focal adhesion, extracellular matrix–receptor interaction, and peroxisome proliferator-activated receptor. The key DEGs in these pathways included ANGPTL4, ACSL5, VEGFC, MAPK10, and CD44. These genes play an important role in the infection of E. tenella. This study further enhances our understanding of the molecular mechanism of E. tenella infection in chickens.

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Artemisinin and Artemisia annua leaves alleviate Eimeria tenella infection by facilitating apoptosis of host cells and suppressing inflammatory response

Jiao

Yang

Liu

et al. 2018

Veterinary Parasitology

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Action of nitromezuril against Eimeria tenella with clinically anticoccidial indices and histopathology

Cited by 5 publications

References 32 publications

Comparison of macroscopy, histopathology and PCR for diagnosing Eimeria spp. in broiler chickens

Comparison of macroscopy, histopathology and PCR for diagnosing Eimeria spp. in broiler chickens

RNA Sequencing Analysis of Chicken Cecum Tissues Following Eimeria tenella Infection in Vivo

Artemisinin and Artemisia annua leaves alleviate Eimeria tenella infection by facilitating apoptosis of host cells and suppressing inflammatory response

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