“…Advanced network analyses based on thresholded pairwise correlations of Ca 2+ imaging signals have proven to be a valuable tool to quantify the non-trivial intercellular interaction patterns in multicellular systems (Stetter, Battaglia et al 2012, Feldt Muldoon, Soltesz et al 2013, including the pancreatic islets (Hodson, Mitchell et al 2013, Stožer, Gosak et al 2013, Markovic, Stozer et al 2015, Johnston, Mitchell et al 2016, Gosak, Markovic et al 2018). These endeavors have been at least in part motivated by the fact that the arrangement and communication abilities between beta cells is an increasingly popular topic in islet and diabetes research, not only because its critical role in insulin release through the generation of coordinated rhythmic activity (Bavamian, Klee et al 2007, Cigliola, Chellakudam et al 2013, Rutter and Hodson 2014, but also due to the growing evidence that connectivity may plausibly be targeted by both environmental and genetic factors in the pathogenesis of diabetes mellitus (Hodson, Mitchell et al 2013, Farnsworth, Walter et al 2016, Westacott, Farnsworth et al 2017). In the present study we pushed further our understanding of beta cell networks by exploring the glucose dependencies of intercellular behavior and by connecting the network properties with cellular signaling characteristics.…”