Phytol induces ROS mediated apoptosis by induction of caspase 9 and 3 through activation of TRAIL, FAS and TNF receptors and inhibits tumor progression factor Glucose 6 phosphate dehydrogenase in lung carcinoma cell line (A549)

Thakor, Parth; Subramanian, R. B.; Thakkar, Sampark S.; Ray, Avik; Thakkar, Vasudev R.

doi:10.1016/j.biopha.2017.05.066

Cited by 70 publications

(28 citation statements)

References 34 publications

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“…TRAIL is specifically bound to the related receptor through a subunit structure composed of homologous trimers in the C terminal of the extracellular domain. The multiple tumor cells and transformed cells can be induced to apoptosis and inhibit through the pathway of caspase, death receptor, and mitochondria-related apoptosis [97]. It is exciting that the apoptosis induced by TRAIL in normal tissue cells of the body have not been confirmed.…”

Section: Trail Expressed On Nk Cells Exerts Anti-hepatocarcinoma Actimentioning

confidence: 99%

Recent Advances in Molecular Mechanisms of the NKG2D Pathway in Hepatocellular Carcinoma

Wang¹,

Li²,

Sun³

2020

Biomolecules

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Hepatocellular carcinoma is a common malignant tumor with high mortality. Its malignant proliferation, invasion, and metastasis are closely related to the cellular immune function of the patients. NKG2D is a key activated and type II membrane protein molecule expressed on the surface of almost all NK cells. The human NKG2D gene is 270 kb long, located at 12p12.3–p13.1, and contains 10 exons and 9 introns. The three-dimensional structure of the NKG2D monomeric protein contains two alpha-helices, two beta-lamellae, and four disulfide bonds, and its’ signal of activation is transmitted mainly by the adaptor protein (DAP). NKG2D ligands, including MICA, MICB, and ULBPs, can be widely expressed in hepatoma cells. After a combination of NKG2D and DAP10 in the form of homologous two polymers, the YxxM motif in the cytoplasm is phosphorylated and then signaling pathways are also gradually activated, such as PI3K, PLCγ2, JNK-cJunN, and others. Activated NK cells can enhance the sensitivity to hepatoma cells and specifically dissolve by releasing a variety of cytokines (TNF-α and IFN-γ), perforin, and high expression of FasL, CD16, and TRAIL. NK cells may specifically bind to the over-expressed MICA, MICB, and ULBPs of hepatocellular carcinoma cells through the surface activating receptor NKG2D, which can help to accurately identify hepatoma, play a critical role in anti-hepatoma via the pathway of cytotoxic effects, and obviously delay the poor progress of hepatocellular carcinoma.

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Section: Trail Expressed On Nk Cells Exerts Anti-hepatocarcinoma Actimentioning

confidence: 99%

Recent Advances in Molecular Mechanisms of the NKG2D Pathway in Hepatocellular Carcinoma

Wang¹,

Li²,

Sun³

2020

Biomolecules

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“…For example, a previous study showed that TGFβ-1 inhibited induced epithelial-mesenchymal transition in an alveolar epithelial cell line (6). Caspase-3 caspase is the most important effect in the process of cell apoptosis, participate in a variety of diseases in development, especially with lung related diseases are closely related (7,8), so we think that caspase-3 in the occurrence and development of pneumonia is very important. The current study reviewed the studies on the prognostic and predictive significances of B cell lymphoma (Bcl)-2 family proteins in lung diseases for its closely related to cell apoptosis (9,10).…”

Section: Introductionmentioning

confidence: 95%

Downregulation of caspase-3 alleviates Mycoplasma pneumoniae-induced apoptosis in alveolar epithelial cells

Shi

2017

Molecular Medicine Reports

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Mycoplasma pneumoniae (M. pneumoniae) infection is closely associated with pneumonia in children. Apoptosis of alveolar epithelial cells is involved in the development of pneumonia in children. The present study aimed to examine how caspase‑3 influences apoptosis rates in M. pneumoniae‑infected alveolar epithelial cells. A549 alveolar epithelial cells were treated with M. pneumoniae, and cells and culture supernatant were collected at different time points. Alterations in apoptosis rates and caspase‑3 mRNA and protein levels were measured for each treatment group. Cell apoptosis was detected using flow cytometry and TUNEL assay, and cell proliferation was detected using Cell Counting Kit‑8 assay. Caspase‑3 expression in A549 cells was inhibited via small interfering RNA (siRNA) knockdown and relative alterations in mRNA and protein levels and apoptosis rates were measured. Cytokine levels were measured using ELISA assay. Apoptosis rates of alveolar epithelial cells increased with prolonged exposure to M. pneumoniae (P<0.05). M. pneumoniae infection increased interleukin (IL)‑4, IL‑6 and IL‑13 levels and reduced IL‑10 levels. Caspase‑3 was upregulated, whereas B cell lymphoma (Bcl)‑2 was downregulated upon M. pneumoniae exposure for 24 h (P<0.05). Following 12 and 24 h of treatment, caspase‑3 levels in the siRNA‑treated cells were decreased compared with control group (P<0.05). M. pneumoniae also significantly altered caspase‑3 and Bcl‑2 protein expression. M. pneumoniae promoted apoptosis in alveolar epithelial cells via activation of the external death receptor pathway. Therefore, M. pneumoniae infection may affect the development of pneumonia in children by regulating caspase‑3 expression and promoting apoptosis.

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“…[6,25] Phytol is believed to have anticancer properties, [26] triggering apoptosis in liver and lung cancer cells activated via caspase 3 and 9 pathway. [27,28] Besides phytol, other prominent compounds such as loliolide, neophytadiene and γsitosterol were found in this study. Loliolide has been reported for its antioxidant [29] and antiproliferative effects.…”

Section: Discussionmentioning

confidence: 50%

Phytochemical Screening and Cytotoxic Effects of Crude Extracts of Pereskia Bleo Leaves

Mohd-Salleh

Ismail

Wan-Ibrahim

et al. 2020

Journal of Herbs, Spices & Medicinal Plants

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The phytocompounds in crude solvent extracts of Pereskia bleo leaves were identified and their cytotoxic effects on cancer cell lines were determined. Crude extracts were obtained via maceration and subjected to GCMS analysis. Then, each extract was incubated with HeLa, MDA-MB-231, SW480, and NIH/3T3 cell lines for 72 h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was done to determine IC 50 values of each extract. Terpenoids, sterols, alkaloids, fatty acids and phenolic compounds were identified from the crude extracts of P. bleo leaves. Other compounds identified were γ-sitosterol, β-tocopherol, and γ-tocopherol. The ethyl acetate extract had potent cytotoxic effect against HeLa and MDA-MB-231 cancer cells as noted by the lowest IC 50 values ARTICLE HISTORY

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Phytol induces ROS mediated apoptosis by induction of caspase 9 and 3 through activation of TRAIL, FAS and TNF receptors and inhibits tumor progression factor Glucose 6 phosphate dehydrogenase in lung carcinoma cell line (A549)

Cited by 70 publications

References 34 publications

Recent Advances in Molecular Mechanisms of the NKG2D Pathway in Hepatocellular Carcinoma

Recent Advances in Molecular Mechanisms of the NKG2D Pathway in Hepatocellular Carcinoma

Downregulation of caspase-3 alleviates Mycoplasma pneumoniae-induced apoptosis in alveolar epithelial cells

Phytochemical Screening and Cytotoxic Effects of Crude Extracts of Pereskia Bleo Leaves

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