Anti-inflammatory effects and corresponding mechanisms of cirsimaritin extracted from Cirsium japonicum var. maackii Maxim

Shin, Myoung‐Sook; Park, Jun Yeon; Lee, Jaemin; Yoo, Hye Hyun; Hahm, Dae‐Hyun; Lee, Sang Cheon; Lee, Sang‐Hyun; Hwang, Gwi Seo; Jung, Kiwon; Kang, Ki Sung

doi:10.1016/j.bmcl.2017.05.051

Cited by 45 publications

(31 citation statements)

References 32 publications

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“…Rosmarinic acid, cirsimaritin, salvigenin, and carvacrol are four constituents of PA-F4. The anti-inflammatory effects of rosmarinic acid, cirsimaritin, and carvacrol but not salvigenin have been studied through the inhibition of NF-κB activation (Shin et al, 2017; Wei et al, 2018; Xiao et al, 2018). Among them, only rosmarinic acid has been reported to suppress the activation of NLRP3 inflammasome (Wei et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Extract From Plectranthus amboinicus Inhibit Maturation and Release of Interleukin 1β Through Inhibition of NF-κB Nuclear Translocation and NLRP3 Inflammasome Activation

2019

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Uncontrolled inflammation may produce massive inflammatory cytokines, in which interleukin 1β (IL-1β) plays a key role, resulting in tissue damage and serious disorders. The activation of NLRP3 inflammasome is one of the major mechanisms in maturation and release of IL-1β. Plectranthus amboinicus is a perennial herb. Several pharmacological activities of natural components and crude extracts from P. amboinicus have been reported including anti-inflammation; however, the underlying mechanism is not clear. Phorbol-12-myristate 13-acetate-differentiated THP-1 monocytic leukemia cells were used as a reliable model in this study to examine the effect on inflammasome signaling pathway by PA-F4, an extract from Plectranthus amboinicus . PA-F4 inhibited ATP-induced release of caspase-1, IL-1β, and IL-18 from lipopolysaccharides (LPS)-primed cells. PA-F4 induced a concentration-dependent inhibition of both ASC dimerization and oligomerization in cells under LPS priming plus ATP stimulation. Co-immunoprecipitation of NLRP3 and ASC demonstrated that PA-F4 significantly blunted the interaction between NLRP3 and ASC. Furthermore, PA-F4 completely abolished ATP-induced K + efflux reaction in LPS-primed cells. Taken together, PA-F4 displayed an inhibitory activity on NLRP3 inflammasome activation. Moreover, PA-F4 also inhibited LPS-induced p65 NF-κB activation, suggesting an inhibitory activity on LPS priming step. Further identification showed that rosmarinic acid, cirsimaritin, salvigenin, and carvacrol, four constituents in PA-F4, inhibited LPS-induced IL-6 release. In contrast, rosmarinic acid, cirsimaritin and carvacrol but not salvigenin inhibited ATP-induced caspase-1 release from LPS-primed cells. In conclusion, PA-F4 displayed an inhibitory activity on activation of NLRP3 inflammasome. PA-F4 inhibited LPS priming step through block of p65 NF-κB activation. It also inhibited ATP-induced signaling pathways in LPS-primed cells including the inhibition of both ASC dimerization and oligomerization, K + efflux reaction, and the release reaction of caspase-1, IL-1β, and IL-18. Rosmarinic acid, cirsimaritin, salvigenin, and carvacrol could partly explain PA-F4-mediated inhibitory activity on blocking the activation of NLRP3 inflammasome.

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Section: Discussionmentioning

confidence: 99%

Extract From Plectranthus amboinicus Inhibit Maturation and Release of Interleukin 1β Through Inhibition of NF-κB Nuclear Translocation and NLRP3 Inflammasome Activation

2019

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“…ussuriense is flavonoids, which comprise of cirsimaritin, 5,7‐dihydroxy‐6,4′‐dimethoxyflavone, hispidulin‐7‐neohesperidoside, and pectolinarin. It was discovered that cirsimaritin exerted anti‐inflammatory effects on lipopolysaccharide‐stimulated RAW264.7 cells via inhibiting IL‐6, tumor necrosis factor‐α, NO, and PGE2 production . Moreover, it was reported that hispidulin‐7‐O‐neohesperidoside had the potential as an anti‐inflammatory agent to prevent and/or treat inflammatory bowel disease .…”

Section: Discussionmentioning

confidence: 99%

Pectolinarin inhibits proliferation, induces apoptosis, and suppresses inflammation in rheumatoid arthritis fibroblast‐like synoviocytes by inactivating the phosphatidylinositol 3 kinase/protein kinase B pathway

Wang

Zhao

et al. 2019

J of Cellular Biochemistry

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Rheumatoid arthritis fibroblast‐like synoviocytes (RA‐FLSs), a pathological hallmark of rheumatoid arthritis (RA), exhibit the characteristics of tumor cells. The extracts of Cirsium japonicum var. ussuriense have been shown to possess antitumor and anti‐inflammatory activities. Our study aimed to investigate the effects of pectolinarin, a flavonoid compound isolated from C. japonicum var. ussuriense, on RA. Cell viability was evaluated by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide assay. Apoptosis was determined by flow cytometry analysis and Western blot analysis of Bax and Bcl‐2 levels. Inflammation was assessed by detecting the expressions and secretion of interleukin (IL)‐6 and IL‐8 using quantitative real‐time polymerase chain reaction and enzyme‐linked immunosorbent assay, respectively. The production of nitric oxide (NO) and prostaglandin E2 (PGE2) was also measured. The effects of pectolinarin on the phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) pathway were examined by Western blot. We found that pectolinarin significantly inhibited cell viability at 24 and 48 hours in a dose‐dependently manner in RA‐FLSs. Pectolinarin reduced the apoptotic rate, increased Bax level, and decreased Bcl‐2 level in RA‐FLSs. Pectolinarin inhibited the messenger RNA expression and secretion of IL‐6 and IL‐8, as well as the production of PGE2 and NO in RA‐FLSs. Furthermore, pectolinarin inactivated the phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) pathway in RA‐FLSs. Activation of the PI3K/Akt pathway by 740Y‐P impaired the effects of pectolinarin on cell viability, apoptosis, and inflammation in RA‐FLSs. In conclusion, pectolinarin suppressed cell proliferation and inflammatory response and induced apoptosis in RA‐FLSs via inactivation of the PI3K/Akt pathway.

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“…According to these data, compounds 3 and 6 are the most potent, having a low DE 50 , while 2 is the least potent. Compounds 6 and 7 are known to display anti-inflammatory activity in croton oil-induced ear edema, which is regulated by the inhibition of interleukin-6, tumor necrosis factor-α, NO production [32], c-fos and STAT3 phosphorylation in a concentration-dependent manner in lipopolysaccharide (LPS)-stimulated RAW264.7 cells [33]. Values are mean ± SEM (n = 5).…”

Section: Anti-inflammatory Effect Of Compounds Of B Confertamentioning

confidence: 99%

Effect of Terpenoids and Flavonoids Isolated from Baccharis conferta Kunth on TPA-Induced Ear Edema in Mice

et al. 2020

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In this study, we isolated from the aerial parts of Baccharis conferta Kunth (i) a new neoclerodane, denominated “bacchofertone”; (ii) four known terpenes: schensianol A, bacchofertin, kingidiol and oleanolic acid; and (iii) two flavonoids: cirsimaritin and hispidulin. All structures were identified by an exhaustive analysis of nuclear magnetic resonance (NMR) and mass spectroscopy (MS). Extracts from aerial parts were screened for anti-inflammatory activity in the mice ear edema model of 12-O-tetradecanoylforbol-13-acetate mice. Dichloromethane extract (BcD) exhibited 78.5 ± 0.72% inhibition of edema, followed by the BcD2 and BcD3 fractions of 71.4% and 82.9% respectively, at a dose of 1 mg/ear. Kingidiol and cirsimaritin were the most potent compounds identified, with a median effective dose of 0.12 and 0.16 mg/ear, respectively. A histological analysis showed that the topical application of TPA promoted intense cell infiltration, and this inflammatory parameter was reduced with the topical application of isolated compounds.

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Anti-inflammatory effects and corresponding mechanisms of cirsimaritin extracted from Cirsium japonicum var. maackii Maxim

Cited by 45 publications

References 32 publications

Extract From Plectranthus amboinicus Inhibit Maturation and Release of Interleukin 1β Through Inhibition of NF-κB Nuclear Translocation and NLRP3 Inflammasome Activation

Extract From Plectranthus amboinicus Inhibit Maturation and Release of Interleukin 1β Through Inhibition of NF-κB Nuclear Translocation and NLRP3 Inflammasome Activation

Pectolinarin inhibits proliferation, induces apoptosis, and suppresses inflammation in rheumatoid arthritis fibroblast‐like synoviocytes by inactivating the phosphatidylinositol 3 kinase/protein kinase B pathway

Effect of Terpenoids and Flavonoids Isolated from Baccharis conferta Kunth on TPA-Induced Ear Edema in Mice

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